A5042660176- Lymphatic System and Diseases
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- S100 Proteins and Annexins
- Sepsis Diagnosis and Treatment
- Sympathectomy and Hyperhidrosis Treatments
- Cell Adhesion Molecules Research
- Planarian Biology and Electrostimulation
- Fatty Acid Research and Health
- Blood Coagulation and Thrombosis Mechanisms
- Blood properties and coagulation
- Neonatal Respiratory Health Research
- Eicosanoids and Hypertension Pharmacology
- Inflammation biomarkers and pathways
- Hemoglobin structure and function
- Erythrocyte Function and Pathophysiology
- Electromagnetic Fields and Biological Effects
- Chronic Kidney Disease and Diabetes
- Cardiovascular and Diving-Related Complications
- Delphi Technique in Research
- Cholesterol and Lipid Metabolism
- Angiogenesis and VEGF in Cancer
- Vascular Malformations and Hemangiomas
- Acute Ischemic Stroke Management
- Barrier Structure and Function Studies
University of South Florida
2017-2023
University of Southampton
2013-2018
Florida College
2017
Endothelial hyperpermeability exacerbates multiple organ damage during inflammation or infection. The endothelial glycocalyx, a protective matrix covering the luminal surface of cells (ECs), undergoes enzymatic shedding inflammation, contributing to barrier hyperpermeability. A disintegrin and metalloproteinase 15 (ADAM15) is sheddase capable cleaving ectodomains membrane-bound molecules. Herein, we tested whether how ADAM15 involved in glycocalyx vascular leakage sepsis. Dextran-150kD...
Lymphatic capillaries develop discontinuous cell-cell junctions that permit the absorption of large macromolecules, chylomicrons, and fluid from interstitium. While excessive vascular endothelial growth factor 2 (VEGFR2) signaling can remodel seal these junctions, whether how VEGFR3 alter lymphatic remains incompletely understood. Here, we use lymphatic-specific Flt4 knockout mice to investigate in junctions. We show loss prevents specialized button junction formation multiple tissues...
Background and Purpose The ω‐6 fatty acid‐derived lipid mediators such as prostanoids, thromboxane leukotrienes have well‐established roles in regulating both inflammation smooth muscle contractility. Resolvins are derived from ω‐3 acids important promoting the resolution of inflammation, but their activity on contractility is unknown. We investigated whether resolvin E1 (RvE1), D1 (RvD1) D2 (RvD2) can modulate contractions isolated segments rat thoracic aorta (RTA) or human pulmonary artery...
Objective The endothelial glycocalyx constitutes part of the barrier but its degradation leaves cells exposed to transmigrating and circulating mediators that can damage or promote intercellular gaps. Syndecan proteins are key components shed during disease states where expression activity proteases such as thrombin elevated. We tested ability cleave ectodomains syndecans whether products could act directly on alter function. Approach results Using transmission electron microscopy, we...
Lymphatic vascular permeability prevents lymph leakage that is associated with lymphedema, lymphatic malformations, obesity, and inflammation. However, the molecular control of remains poorly understood. Recent studies have suggested adherens junctions vesicle transport may be involved in regulating vessel permeability. To determine contribution each pathway, we utilized an ex vivo assay to directly measure solute flux various weight solutes across a range pressures intact murine collecting...
Neutrophil extracellular traps (NETs) are DNA‐webs containing histones and granular enzymes produced by neutrophils during host defense against infection. It is becoming increasingly apparent that circulating NETs exacerbate tissue injury associated with inflammation. The purpose of this study was to determine the effects on endothelial barrier function. We identified increased markers in lung plasma mice subjected polymicrobial septic induced cecal ligation puncture. In an intravital...
Thromboxane is a vasoactive omega-6-derived lipid implicated in hypertension. Omega-3 fatty acid derivatives including resolvin (Rv) E1 have important roles the resolution of inflammation, but their ability to mediate vasomotor activity unknown. We assessed whether RvE1 modulates contraction rat thoracic aorta and human pulmonary artery (HPA) vitro. Rats were culled by CO2 inhalation cervical dislocation, was removed. HPA obtained with informed consent from lung tissue surgical patients at...
Endothelial surface glycocalyx composed of glycosaminoglycans, proteoglycans and glycoproteins plays an important role in regulating vascular barrier integrity. A disintegrin metalloprotease 15 (ADAM15) is a membrane‐associated sheddase which has recently been characterized as mediator endothelial hyperpermeability during inflammation. This study tested the hypothesis that ADAM15 contributes to microvascular dysfunction by damaging Intravital microscopic analysis mouse mesenteric...
The endothelial glycocalyx (GCX) is a complex matrix of glycosaminoglycans and proteoglycans such as syndecans (SDCs), which form protective layer on the vascular luminal surface contributing to barrier function. Ectodomain shedding transmembrane or degradation GCX components can damage integrity. objective this study was investigate involvement disintegrin metalloproteinase 15 (ADAM15) in shedding, effects SDC fragments A confocal microscopic imaging analysis mouse lungs demonstrated...
The endothelial glycocalyx (eGCX) is a protective barrier on the luminal surface of cells. syndecan family proteoglycans constitute integral components eGCX and can act as receptors for many cell signaling pathways. Under inflammatory conditions, be cleaved shed by proteases such matrix metalloproteinases thrombin. We analyzed ability thrombin to cleave core proteins from microvessels in human lung samples using transmission electron microscopy (TEM) immunogold staining syndecan‐4 ectodomain...
Disruption of vascular endothelial barrier results in excessive accumulation plasma proteins and fluid within the interstitial space, exacerbating tissue injury caused by infection or sepsis. There is an urgent need to explore effective strategies restore integrity patients with Glycocalyx, surface coating matrix composed glycoproteins proteinglycans attached glycoaminoglycan chains, has been shown be protective function. Degradation glycocalyx implicated progression septic injury, evidenced...
Circulating extracellular histones are known to contribute tissue injury in systemic inflammatory responses infection, trauma, and burns. The mechanisms of histone modifications their effects on inflammation currently being investigated. One such modification, namely citrullination 3, is catalyzed by the peptidylarginine deiminase 4 (PAD4) enzyme thought initiate process NETosis (the release neutrophil traps) response infection. NETs composed a chromatin web with attached proteases histones,...