Environmental stresses such as drought, salinity, and cold are major factors that significantly limit agricultural productivity.NAC transcription play essential roles in response to various abiotic stresses.However, the paucity of wheat NAC members functionally characterized date does not match importance this plant a world staple crop.Here, function TaNAC2 was Arabidopsis thaliana.A fragment obtained from suppression subtractive cDNA libraries treated with polyethylene glycol, its...

Osmotic stresses such as drought, salinity, and cold are major environmental factors that limit agricultural productivity worldwide. Protein phosphorylation/dephosphorylation signalling events induced by osmotic stress in higher plants. Sucrose non-fermenting 1-related protein kinase2 family members play essential roles response to hyperosmotic Arabidopsis, rice, maize. In this study, the function of TaSnRK2.4 salt, freezing Arabidopsis was characterized. A translational fusion with green...

Drought, salinity and low temperatures are major factors limiting crop productivity quality. Sucrose non-fermenting1-related protein kinase 2 (SnRK2) plays a key role in abiotic stress signaling plants. In this study, TaSnRK2.8, SnRK2 member wheat, was cloned its functions under multi-stress conditions were characterized. Subcellular localization showed the presence of TaSnRK2.8 cell membrane, cytoplasm nucleus. Expression pattern analyses wheat revealed that involved response to PEG, NaCl...

Abiotic stresses are major environmental factors that affect agricultural productivity worldwide. NAC transcription play pivotal roles in abiotic stress signaling plants. As a staple crop, wheat production is severely constrained by whereas only few have been characterized functionally. To promote the application of genes improvement biotechnology, novel gene designated TaNAC67 was common wheat. determine its role, transgenic Arabidopsis overexpressing TaNAC67-GFP controlled CaMV-35S...

Calreticulin (CRT) is a highly conserved and ubiquitously expressed Ca2+-binding protein in multicellular eukaryotes. CRT plays crucial role many cellular processes including Ca2+ storage release, synthesis, molecular chaperone activity. To elucidate the function of CRTs plant responses against drought, main abiotic stress limiting cereal crop production worldwide, full-length cDNA encoding calreticulin namely TaCRT was isolated from wheat (Triticum aestivum L.). The deduced amino acid...

High yield and wide adaptation are principal targets of wheat breeding but hindered by limited knowledge on genetic basis agronomic traits abiotic stress tolerances. In this study, 277 accessions were phenotyped across 30 environments with non-stress, drought-stressed, heat-stressed, drought-heat-stressed treatments subjected to genome-wide association study using 395 681 single nucleotide polymorphisms. We detected 295 associated loci including consistent for different eurytopic multiple A...

Sucrose non-fermenting-1-related protein kinase 2 (SnRK2) plays a key role in the plant stress signalling transduction pathway via phosphorylation. Here, SnRK2 member of common wheat, TaSnRK2.7, was cloned and characterized. Southern blot analysis suggested that wheat genome contains three copies TaSnRK2.7. Subcellular localization showed presence TaSnRK2.7 cell membrane, cytoplasm, nucleus. Expression patterns revealed is expressed strongly roots, responds to polyethylene glycol, NaCl, cold...

Abstract Background and Aims Root system morphology is important for sustainable agriculture, but the genetic basis of root traits their relationship to shoot remain be elucidated. The aim present study was dissect at late growth stages its implications on in wheat. Methods Among 323 wheat accessions, we investigated phenotypic differences booting mid-grain fill PVC tubes, including plant height (PH), canopy temperature (CT) grain yield per (YPP) a field experiment, performed genome-wide...

Environmental stresses such as drought, salinity, and cold are major adverse factors that significantly affect agricultural productivity. Protein phosphorylation/dephosphorylation is a signalling event induced by osmotic stress in higher plants. Sucrose non-fermenting 1-related protein kinase 2 (SnRK2) family members play essential roles the response to hyperosmotic In this study, TaSnRK2.3 gene, novel SnRK2 member was cloned, three copies located on chromosomes 1A, 1B, 1D were identified...

AREB (ABA response element binding) proteins in plants play direct regulatory roles to multiple stresses, but their functions wheat (Triticum aestivum L.) are not clear.In the present study, TaAREB3, a new member of transcription factor family, was isolated from wheat.Sequence analysis showed that TaAREB3 protein is composed three parts, conserved N-terminal, variable M region, and C-terminal with bZIP domain.It belongs group A subfamily factors.TaAREB3 constitutively expressed stems,...

Axillary meristems play an important role in determining final plant architecture and floral structures. Tomato Ls, Arabidopsis LAS rice MOC1 are orthologous genes regulating axillary meristem initiation outgrowth. Their functions generally conserved but the functional specificities divergent among species. Obvious differences between panicles wheat spikes suggest of its ortholog. We show that TaMOC1 might be involved spikelet development. is a typical nucleus localized protein with...

Plant responses to stress occur via abscisic acid (ABA) dependent or independent pathways. Sucrose non-fermenting1-related protein kinase 2 (SnRK2) play a key role in plant signal transduction It is known that some SnRK2 members are positive regulators of ABA through interaction with group A type 2C phosphatases (PP2Cs). Here, 10 SnRK2s were isolated from wheat. Based on phylogenetic analysis using domains the C-terminus, divided into three subclasses. Expression pattern revealed all...

Drought seriously impacts wheat production (Triticum aestivum L.), while the exploitation and utilization of genes for drought tolerance are insufficient. Leaf wilting is a direct reflection in plants. Clade A PP2Cs abscisic acid (ABA) co-receptors playing vital roles ABA signaling pathway, regulating response. However, other clade tolerance, especially wheat, remain largely unknown. Here, we identified gain-of-function drought-induced 1 (DIW1) gene from Aikang 58 mutant library by map-based...

Abiotic stresses such as drought, salinity, and low temperature have drastic effects on plant growth development. However, the molecular mechanisms regulating biochemical physiological changes in response to are not well understood. Protein kinases major signal transduction factors among reported mediating acclimation environmental changes. kinase ABC1 (activity of bc1 complex) is involved coenzyme Q biosynthesis mitochondria yeast (Saccharomyces cersvisiae), balancing oxidative stress...

Improved root architecture is an effective strategy to increase crop yield. We demonstrate that overexpression of transcription factor gene MORE ROOT (TaMOR) from wheat (Triticum aestivum L.) results in more roots and higher grain yield rice (Oryza sativa). TaMOR, encoding a plant-specific belonging the ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES (AS2/LOB) protein family, highly conserved its wild relatives. In this study, tissue expression patterns indicated TaMOR mainly localizes...

Auxin response factors (ARFs) as transcription activators or repressors have important roles in plant growth and development, but knowledge about the functions of wheat ARF members is limited. A novel member (Triticum aestivum), TaARF4, was identified, its protein function, haplotype geographic distribution allelic frequencies were investigated.Tissue expression TaARF4 analysed by real-time PCR. Sub-cellular localization performed using green fluorescent (GFP)-tagged TaARF4. Ectopic TaARF4-A...

We developed breeder-friendly high-throughput and cost-effective KASP marker for marker-assisted selection grain yield related traits in wheat. Plant-specific protein kinase, SnRK2s, is a major family of signaling genes associated with metabolic regulations, nutrient utilization response to external stimuli. In the present study, three copies TaSnRK2.9 were isolated from chromosomes 5A, 5B 5D wheat (Triticum aestivum L.). The coding regions TaSnRK2.9-5A, TaSnRK2.9-5B promoter region...

Salt and drought-induced RING finger1 (SDIR1) is a RING-type E3 ubiquitin ligase that plays key role in ABA-mediated responses to salinity drought stress via the ubiquitination pathway some plant species. However, its function wheat (Triticum aestivum) unknown. Here, we isolated SDIR1 member wheat, TaSDIR1-4A, characterized activity. DNA polymorphism assays showed presence of two nucleotide variation sites promoter region leading detection haplotypes Hap-4A-1 Hap-4A-2 populations....

Abstract Wheat (Triticum aestivum) is particularly susceptible to water deficit at the jointing stage of its development. Sucrose non-fermenting 1-related protein kinase 2 (SnRK2) acts as a signaling hub in response drought stress, but whether SnRK2 helps plants cope with via other mechanisms largely unknown. Here, we cloned and characterized TaSnRK2.10, which was induced by multiple abiotic stresses phytohormones. Ectopic expression TaSnRK2.10 rice (Oryza sativa) conferred tolerance,...