A5028310890- Phytochemical Studies and Bioactivities
- Natural product bioactivities and synthesis
- Liver Disease Diagnosis and Treatment
- Receptor Mechanisms and Signaling
- Chromatography in Natural Products
- Drug-Induced Hepatotoxicity and Protection
- Diet, Metabolism, and Disease
- Liver physiology and pathology
- Genomics, phytochemicals, and oxidative stress
- Pharmacological Effects of Natural Compounds
- Traditional Chinese Medicine Analysis
- Neuropeptides and Animal Physiology
- Liver Disease and Transplantation
- Eicosanoids and Hypertension Pharmacology
- Neurological Disease Mechanisms and Treatments
- Enzyme Structure and Function
- Monoclonal and Polyclonal Antibodies Research
- Bioactive natural compounds
- SARS-CoV-2 and COVID-19 Research
- MicroRNA in disease regulation
- Neuroinflammation and Neurodegeneration Mechanisms
- Photosynthetic Processes and Mechanisms
- Nitric Oxide and Endothelin Effects
- Phytochemistry and Biological Activities
- Metabolomics and Mass Spectrometry Studies
Shanghai Institute of Materia Medica
2021-2025
Chinese Academy of Sciences
2021-2025
Guizhou University
2025
University of Chinese Academy of Sciences
2025
Shenzhen Institutes of Advanced Technology
2022-2024
Dalian Medical University
2013-2023
ShanghaiTech University
2018-2023
Max Planck Institute for Biophysical Chemistry
2017
Max Planck Society
2017
Shandong University
2017
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant has become the dominant infective strain. We report structures of spike trimer on its own and in complex with angiotensin-converting enzyme (ACE2) or an anti-Omicron antibody. Most mutations are located surface protein change binding epitopes to many current antibodies. In ACE2-binding site, compensating strengthen receptor domain (RBD) ACE2. Both RBD apo form thermodynamically unstable. An unusual RBD-RBD...
A SERS based biosensor has been developed for isolation, detection and killing of multiple bacterial pathogens.
Abstract The Omicron BA.2 variant has become a dominant infective strain worldwide. Receptor binding studies show that the spike trimer exhibits 11-fold and 2-fold higher potency in to human ACE2 than from wildtype (WT) BA.1 strains. structure of complexed with reveals all three receptor-binding domains (RBDs) are open conformation, ready for binding, thus providing basis increased infectivity strain. JMB2002, therapeutic antibody was shown efficiently inhibit BA.1, also shows potent...
Abstract The adenosine A 3 receptor (A AR), a key member of the G protein-coupled family, is promising therapeutic target for inflammatory and cancerous conditions. selective AR agonists, CF101 CF102, are clinically significant, yet their recognition mechanisms remained elusive. Here we report cryogenic electron microscopy structures full-length human bound to CF102 with heterotrimeric i protein in complex at 3.3-3.2 Å resolution. These agonists reside orthosteric pocket, forming conserved...
Abstract The present work aimed to investigate the activities and underlying mechanisms of dioscin against alcoholic liver fibrosis (ALF). In vivo in mice was induced by an liquid diet vitro studies were performed on activated HSC-T6 LX2 cells treated with lipopolysaccharide. Our results showed that significantly attenuated hepatic stellate (HSCs) activation, improved collagen accumulation inflammation through down-regulating levels myeloid differentiation factor 88 (MyD88), nuclear κB...
The oncogenic fusion protein BCR-ABL is the driving force of leukemogenesis in chronic myeloid leukemia (CML). Despite great progress for CML treatment through application tyrosine kinase inhibitors (TKIs) against BCR-ABL, long-term drug administration and clinical resistance continue to be an issue. Herein, we described design, synthesis, evaluation novel proteolysis-targeting chimeric (PROTAC) small molecules targeting which connect dasatinib VHL E3 ubiquitin ligase ligand by extensive...
Background and Purpose Dioscin exhibits a range of pharmacological actions but little is known its effects on cisplatin (CDDP)‐induced nephrotoxicity. Here, we have assessed the possible mechanisms dioscin against CDDP‐induced Experimental Approach We used an in vivo model nephrotoxicity rats mice and, vitro, cultures NRK‐52E HK‐2 cells. The dual luciferase reporter assay was to demonstrate modulation, by dioscin, targeting sirtuin 1 (Sirt1) microRNA (miR)‐34a. Molecular docking assays were...
The mechanisms of the natural product dioscin against non-alcoholic fatty liver disease (NAFLD) are unclear. Thus, purpose present study was to further confirm its effects prevention and then elucidate potential underlying activity in mice. High-fat diet (HFD)-induced C57BL/6J mice ob/ob were used as experimental models. Serum hepatic biochemical parameters determined mRNA protein expression levels detected. results indicated that alleviated body weight lipid accumulation symptoms, increased...
The conserved polymerase-associated factor 1 complex (Paf1C) plays multiple roles in chromatin transcription and genomic regulation. Paf1C comprises the five subunits Paf1, Leo1, Ctr9, Cdc73 Rtf1, binds to RNA polymerase II (Pol II) elongation (EC). Here we report reconstitution of from Saccharomyces cerevisiae, a structural analysis bound Pol EC containing TFIIS. Cryo-electron microscopy crosslinking data reveal that is highly mobile extends over outer surface Rpb2 Rpb3 subunit. Paf1-Leo1...
Abstract We previously reported the promising effects of dioscin against liver injury, but its effect on fibrosis remains unknown. The present work investigated activities and underlying molecular mechanisms. Dioscin effectively inhibited cell viabilities HSC-T6, LX-2 primary rat hepatic stellate cells (HSCs), not hepatocytes. Furthermore, markedly increased peroxisome proliferator activated receptor-γ (PPAR-γ) expression significantly reduced a-smooth muscle actin (α-SMA), transforming...