A5087912904- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- Cell Image Analysis Techniques
- Retinal Development and Disorders
- Genomics and Chromatin Dynamics
- Neuroinflammation and Neurodegeneration Mechanisms
- Histone Deacetylase Inhibitors Research
- Neuroscience and Neuropharmacology Research
- Cancer-related gene regulation
- Gene expression and cancer classification
- Bioinformatics and Genomic Networks
- Advanced Fiber Optic Sensors
- Vector-borne infectious diseases
- Biomedical and Engineering Education
- Bacillus and Francisella bacterial research
- Genetics and Neurodevelopmental Disorders
- Olfactory and Sensory Function Studies
- Photonic and Optical Devices
- Kruppel-like factors research
- Health and Medical Research Impacts
- Circadian rhythm and melatonin
- RNA modifications and cancer
- Yersinia bacterium, plague, ectoparasites research
- Semiconductor Lasers and Optical Devices
Brandeis University
2015-2025
Brigham and Women's Hospital
2010-2019
Harvard University
2010-2019
Translational Genomics Research Institute
2007
Understanding the principles governing neuronal diversity is a fundamental goal for neuroscience. Here, we provide an anatomical and transcriptomic database of nearly 200 genetically identified cell populations. By separately analyzing robustness pattern expression differences across these populations, identify two gene classes contributing distinctly to diversity. Short homeobox transcription factors distinguish populations combinatorially, exhibit extremely low transcriptional noise,...
Abstract A critical phase of mammalian brain development takes place after birth. Neurons the mouse neocortex undergo dramatic changes in their morphology, physiology, and synaptic connections during first postnatal month, while properties immature neurons, such as capacity for robust axon outgrowth, are lost. The genetic epigenetic programs controlling prenatal well studied, but our understanding transcriptional mechanisms that regulate neuronal maturation is comparatively lacking. By...
Retinoic acid-related orphan receptor beta (RORβ) is a transcription factor (TF) and marker of layer 4 (L4) neurons, which are distinctive both in transcriptional identity the ability to form aggregates such as barrels rodent somatosensory cortex. However, relationship between L4 cytoarchitecture largely unknown. We find RORβ required cortex for aggregation into thalamocortical afferent (TCA) segregation. Interestingly, barrel organization also degrades with age wildtype mice. Loss delays...
There is a pressing need to increase the rigor of research in life and biomedical sciences. To address this issue, we propose that communities 'rigor champions' be established campaign for reforms culture has led shortcomings rigor. These champions would also assist development adoption comprehensive educational platform teach principles rigorous science researchers at all career stages.
BackgroundYersinia pestis, the causative agent of plague, is responsible for some greatest epidemic scourges mankind. It widespread in western United States, although it has only been present there just over 100 years. As a result, very little time diversity to accumulate this region. Much that detected among North American isolates at loci mutate too quickly accurately reconstruct large-scale phylogenetic patterns. Slowly-evolving but stable markers such as SNPs could be useful purpose, are...
Transcription regulation is a key aspect of cellular identity established during development and maintained into adulthood. Molecular biochemical assays that probe the genome are critical tools in exploring mechanisms transcription cell type identity. The mammalian brain composed huge diversity types with distinct properties functions. To understand these specific roles, it necessary to selectively target populations for study. However, need study restricted poses challenge neurobiology. It...
Abstract The mammalian nervous system is constructed of many cell types, but the principles underlying this diversity are poorly understood. To assess brain-wide transcriptional diversity, we sequenced transcriptomes largest collection genetically and anatomically identified neuronal classes. Using improved expression metrics that distinguish information content from signal-to-noise-ratio, found homeobox transcription factors contain highest about types have lowest noise. Genes contribute...
Abstract Retinoic Acid-Related Orphan Receptor Beta (RORβ) is a transcription factor (TF) and marker of layer 4 (L4) neurons, which are distinctive both in transcriptional identity the ability to form aggregates such as barrels rodent somatosensory cortex. However, relationship between L4 cytoarchitecture largely unknown. We find RORβ required cortex for aggregation into thalamocortical afferent (TCA) segregation. Interestingly, barrel organization also degrades with age. Loss delays...