A5005060100- Single-cell and spatial transcriptomics
- Neuroscience and Neuropharmacology Research
- Wnt/β-catenin signaling in development and cancer
- Neural dynamics and brain function
- Axon Guidance and Neuronal Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell Image Analysis Techniques
- Hippo pathway signaling and YAP/TAZ
- Photoreceptor and optogenetics research
- Vestibular and auditory disorders
- Memory and Neural Mechanisms
- Developmental Biology and Gene Regulation
- Adipose Tissue and Metabolism
- Cellular Mechanics and Interactions
- Olfactory and Sensory Function Studies
- Zebrafish Biomedical Research Applications
- CRISPR and Genetic Engineering
- Neurobiology and Insect Physiology Research
- Cell Adhesion Molecules Research
- Gene expression and cancer classification
- Moyamoya disease diagnosis and treatment
- Skin and Cellular Biology Research
- Ion Transport and Channel Regulation
- Hearing, Cochlea, Tinnitus, Genetics
- Genomics and Chromatin Dynamics
RIKEN
2023
RIKEN Center for Brain Science
2023
RIKEN Center for Biosystems Dynamics Research
2022
Brandeis University
2011-2020
Hirosaki University
2011
University of Manchester
2011
Kyoto University
1999-2008
Osaka Dental University
1967
Cerebellar granule cells constitute the majority of neurons in brain and are primary conveyors sensory motor-related mossy fiber information to Purkinje cells. The functional capability cerebellum hinges on whether individual receive inputs from multiple precerebellar nuclei or instead unimodal; this distinction is unresolved. Using cell-type-specific projection mapping with synaptic resolution, we observed convergence separate (upper body proprioceptive) basilar pontine pathways onto mapped...
Significance The dopamine-containing nigrostriatal system and its return striatonigral pathway form a loop–circuit crucial for the functions of dopamine in modulating movement mood. Here we identify specialized subsystem within this loop. With new mouse models tissue expansion to allow nanoscale imaging, demonstrate that fibers originating striosomes bouquet-like arborizations innervating clusters neurons their ventrally extending, tightly bundled dendrites. Within these formations (termed...
Understanding the principles governing neuronal diversity is a fundamental goal for neuroscience. Here, we provide an anatomical and transcriptomic database of nearly 200 genetically identified cell populations. By separately analyzing robustness pattern expression differences across these populations, identify two gene classes contributing distinctly to diversity. Short homeobox transcription factors distinguish populations combinatorially, exhibit extremely low transcriptional noise,...
There is a continuing need for driver strains to enable cell-type-specific manipulation in the nervous system. Each cell type expresses unique set of genes, and recapitulating expression marker genes by BAC transgenesis or knock-in has generated useful transgenic mouse lines. However, since are often expressed many types, these lines have relatively broad patterns. We report an alternative approach capturing distal enhancers more focused expression. identified enhancer trap probe producing...
Abstract Background: Cofilin, a key regulator of actin filament dynamics, is inactivated by phosphorylation at Ser‐3 LIM‐kinases and reactivated dephosphorylation family protein phosphatases, termed Slingshot (SSH). Results: We have identified two novel isoforms SSHs, SSH‐2L SSH‐3L characterized them in comparison with SSH‐1L that was previously reported. SSH‐2L, but not SSH‐3L, tightly bound to co‐localized filaments. When expressed cultured cells, SSH‐1L, decreased the level...
Intracranial aneurysms (IAs) are a high-risk factor for life-threatening subarachnoid hemorrhage. Their etiology, however, remains mostly unknown at present. We conducted screening sporadic somatic mutations in 65 IA tissues (54 saccular and 11 fusiform aneurysms) paired blood samples by whole-exome targeted deep sequencing. identified multiple signaling genes examined their impact on downstream pathways gene expression vitro an arterial dilatation model mice vivo. 16 that were mutated least...
Abstract Drosophila Flamingo (Fmi) is an evolutionally conserved seven‐pass transmembrane receptor of the cadherin superfamily. Fmi plays multiple roles in patterning neuronal processes and epithelial planar cell polarity. To explore vivo homologs mammals, we previously cloned one mouse homologs, flamingo1/Celsr2 . Here, report results our study its embryonic postnatal expression patterns together with those two other paralogs, Celsr1 Celsr3 Celsr1–3 was initiated broadly nervous system at...
The broadly-distributed, non-topographic projections to and from the olfactory cortex may suggest a flat, non-hierarchical organization in odor information processing. Layer 2 principal neurons anterior piriform (APC) can be divided into subtypes: semilunar (SL) superficial pyramidal (SP) cells. Although it is known that SL SP cells receive differential inputs bulb (OB), little about their other regions. Here, we examined axonal of using combination mouse genetics retrograde labeling....
The paraventricular nucleus of the thalamus (PVT) projects axons to multiple areas, mediates a wide range behaviors, and exhibits regional heterogeneity in both functions axonal projections. Still, questions regarding cell types present PVT extent their differences remain inadequately addressed. We applied single-cell RNA sequencing depict transcriptomic characteristics mouse neurons. found that one most significant variances transcriptome corresponded anterior-posterior axis. While...
Despite its comparatively simple trilaminar architecture, the primary olfactory (piriform) cortex of mammals is capable performing sophisticated sensory processing, an ability that thought to depend critically on extensive associational (intracortical) excitatory circuits. Here, we used a novel transgenic mouse model and optogenetics measure connectivity circuits originate in semilunar (SL) cells layer 2a anterior piriform (aPC). We generated line (48L) which channelrhodopsin-2 (ChR) could...
Synaptic scaling is a form of homeostatic plasticity driven by transcription-dependent changes in AMPA-type glutamate receptor (AMPAR) trafficking. To uncover the pathways involved, we performed cell-type-specific screen for transcripts persistently altered during scaling, which identified μ subunit (μ3A) adaptor protein complex AP-3A. increased μ3A (but not other AP-3 subunits) pyramidal neurons and redistributed dendritic AMPAR to recycling endosomes (REs). Knockdown prevented synaptic...
Neuronal cell identity is established during development and must be maintained throughout an animal’s life (Fishell G, Heintz N. Neuron 80: 602–612, 2013). Transcription factors critical for establishing neuronal can required maintaining it (Deneris ES, Hobert O. Nat Neurosci 17: 899–907, 2014). Posttranscriptional regulation also plays important role in differentiation (Bian S, Sun T. Mol Neurobiol 44: 359–373, 2011), but its less established. To better understand how posttranscriptional...
Conditioned taste aversion (CTA) is a form of one-trial learning dependent on basolateral amygdala projection neurons (BLApn). Its underlying cellular and molecular mechanisms remain poorly understood. RNAseq from BLApn identified changes in multiple candidate learning-related transcripts including the expected immediate early gene Fos Stk11, master kinase AMP-related pathway with important roles growth, metabolism development, but not previously implicated learning. Deletion Stk11 blocked...
Actin-depolymerizing factor (ADF) and cofilin constitute a family of key regulators actin filament dynamics. ADF/cofilin is inactivated by phosphorylation at Ser-3 LIM-kinases reactivated dephosphorylation Slingshot (SSH) phosphatases. Defects in LIM kinases or have been implicated morbidity human mice; however, the roles mammalian SSH vivo not addressed. In this study, we examined endogenous expression each mouse member various cell lines tissues, showed that SSH-3L protein was strongly...
Acetoacetyl-CoA thiolase (AT) is an enzyme that catalyses the CoA-dependent thiolytic cleavage of acetoacetyl-CoA to yield 2 molecules acetyl-CoA, or reverse condensation reaction.A full-length cDNA clone pBSGT-3, which has homology known thiolases, was isolated from Dictyostelium library.Expression protein encoded in pBSGT-3 Escherichia coli, its activity, and amino acid sequence search revealed encodes AT.The recombinant AT (r-thiolase) expressed active form E. coli expression system,...
Toothbrushing habits were studied by chronocyclograph and EMG as to the movements o upper extremity muscles. Quantitative determinations successfully made of toothbrushing force contact pressure toothbrush against teeth.In a with manual horizontally or stiff bristle toothbrush, M. deltoideus got markedly contracted. The rolling method use soft brush was accompanied active exertion flexor carpi radialis pollicis brevis.Toothbrushing changes depending upon kind used locality brushing well...
Transcription regulation is a key aspect of cellular identity established during development and maintained into adulthood. Molecular biochemical assays that probe the genome are critical tools in exploring mechanisms transcription cell type identity. The mammalian brain composed huge diversity types with distinct properties functions. To understand these specific roles, it necessary to selectively target populations for study. However, need study restricted poses challenge neurobiology. It...
Abstract The mammalian nervous system is constructed of many cell types, but the principles underlying this diversity are poorly understood. To assess brain-wide transcriptional diversity, we sequenced transcriptomes largest collection genetically and anatomically identified neuronal classes. Using improved expression metrics that distinguish information content from signal-to-noise-ratio, found homeobox transcription factors contain highest about types have lowest noise. Genes contribute...