A5063180778- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Neuroinflammation and Neurodegeneration Mechanisms
- Genetics and Neurodevelopmental Disorders
- Axon Guidance and Neuronal Signaling
- Memory and Neural Mechanisms
- Autism Spectrum Disorder Research
- Congenital heart defects research
- Retinal Development and Disorders
- Developmental Biology and Gene Regulation
- RNA Research and Splicing
- Photoreceptor and optogenetics research
- Genetics, Aging, and Longevity in Model Organisms
- FOXO transcription factor regulation
- RNA Interference and Gene Delivery
- Nerve injury and regeneration
- Pluripotent Stem Cells Research
- Neurobiology and Insect Physiology Research
- Advanced Memory and Neural Computing
- Olfactory and Sensory Function Studies
- Receptor Mechanisms and Signaling
- MicroRNA in disease regulation
- GABA and Rice Research
- CRISPR and Genetic Engineering
Gunma University
2022-2025
New York University
2007-2024
Tokyo Women's Medical University
2018-2024
Broad Institute
2018
Harvard University
2018
NYU Langone Health
2012
Columbia University Irving Medical Center
2011
University School
2010
Kyoto University
2001-2004
Kyoto University Hospital
2004
By combining an inducible genetic fate mapping strategy with electrophysiological analysis, we have systematically characterized the populations of cortical GABAergic interneurons that originate from caudal ganglionic eminence (CGE). Interestingly, compared medial (MGE)-derived interneuron populations, initiation [embryonic day 12.5 (E12.5)] and peak production (E16.5) this embryonic structure occurs 3 d later in development. Moreover, unlike either pyramidal cells or MGE-derived...
Inhibitory GABAergic interneurons of the mouse neocortex are a highly heterogeneous population neurons that originate from ventral telencephalon and migrate tangentially up into developing cortical plate. The majority arise transient embryonic structure known as medial ganglionic eminence (MGE), but how remarkable diversity is specified in this region not known. We have taken genetic fate mapping strategy to elucidate temporal origins interneuron subtypes within MGE. used an inducible form...
Ventral telencephalic progenitors expressing the homeodomain transcription factor Nkx6-2 have been shown to give rise a multitude of cortical interneuron subtypes usually associated with origin in either medial ganglionic eminence or caudal eminence.The function directing fate those has, however, not thoroughly analyzed.We used combination genetic inducible mapping and vivo lossof-function analyze requirement determining interneurons.We found that are born characteristic temporal...
It is of considerable interest to determine how diverse subtypes γ-aminobutyric acidergic (GABAergic) interneurons integrate into the functional network cerebral cortex. Using inducible in vivo genetic fate mapping approaches, we found that interneuron precursors arising from medial ganglionic eminence (MGE) and caudal (CGE) at E12.5, respectively, populate deep superficial cortical layers a complementary manner mature These age-matched populations initiate tangential migration cortex...
The neural basic helix-loop-helix transcription factor Ascl1 (previously Mash1) is present in ventricular zone cells restricted domains throughout the developing nervous system. This study uses genetic fate mapping to define stage and lineages spinal cord that are derived from Ascl1-expressing cells. We find progenitors both neurons oligodendrocytes, but not astrocytes. Temporal control of fate-mapping paradigm reveals rapid cell-cycle exit differentiation At embryonic day 11, identifies...
GABAergic interneurons critically regulate cortical computation through exquisite spatiotemporal control over excitatory networks. Precision of this inhibitory requires a remarkable diversity within interneuron populations that is largely specified during embryogenesis. Although expressing the neuronal isoform nitric oxide synthase (nNOS) constitute largest hippocampal cohort their origin and specification remain unknown. Thus, as neurogliaform cells (NGC) Ivy (IvC) represent main nNOS +...
The olfactory bulb (OB) is one of the two major loci in mammalian brain where newborn neurons are constantly integrated into neural circuit during postnatal life. Newborn generated from stem cells subventricular zone (SVZ) lateral ventricle and migrate to OB through rostral migratory stream. majority these differentiate inhibitory interneurons, such as granule periglomerular cells. It has been reported that prolonged supply leads continuous addition/turnover interneuronal populations...
Neurogliaform (RELN+) and bipolar (VIP+) GABAergic interneurons of the mammalian cerebral cortex provide critical inhibition locally within superficial layers. While these subtypes are known to originate from embryonic caudal ganglionic eminence (CGE), specific genetic programs that direct their positioning, maturation, integration into cortical network have not been elucidated. Here, we report in mice expression transcription factor Prox1 is selectively maintained postmitotic CGE-derived...
Background: Whereas Notch signalling is essential for somitogenesis, mice deficient the basic helix‐loop‐helix (bHLH) genes Hes1 and Hes5 , downstream effectors, display normal somite formation, indicating that there may be an as‐yet unidentified ‐related bHLH gene. Results: We identified a novel gene, designated Hes7 from mouse embryos. has conserved domain in amino‐terminal region WRPW at carboxy‐terminal end, like Hes1. The gene located next to Aloxe3 which mapped position 37.0 cM...
Retinal precursor cells give rise to six types of neurons and one type glial cell during development, this process is controlled by multiple basic helix-loop-helix (bHLH) genes. However, the precise mechanism for specification retinal neuronal subtypes, particularly horizontal photoreceptors, remains be determined. Here, we examined retinas with three different combinations triple bHLH gene mutations. In lacking genes Ngn2, Math3, NeuroD, as well other such bipolar were severely decreased in...
Abstract Abnormalities in GABAergic inhibitory circuits have been implicated the aetiology of autism spectrum disorder (ASD). ASD is caused by genetic and environmental factors. Several genes associated with syndromic forms ASD, including FOXG1 . However, when how dysregulation can result defects circuit development ASD-like social impairments unclear. Here, we show that increased or decreased FoxG1 expression both excitatory neurons results ASD-related behavior deficits our mouse models. We...
Autism spectrum disorder (ASD) is characterized by social deficits and restricted behaviors, with developmental defects in GABAergic circuits proposed as a key underlying etiology. Here, we introduce the V-Y assay, novel space preference test which one arm of Y-maze initially hidden later revealed space. Using an ASD mouse model FOXG1 haploinsufficiency, exhibits ASD-like impairments that can be either exacerbated or ameliorated circuit manipulations, observed impaired exploratory behavior...
Members of a subclass hairy/Enhancer split [E(spl)] homologs, called hesr genes, are structurally related to another hairy/E(spl) Hes which play an important role in neural development. To characterize the roles genes development, we used retina as model system. In situ hybridization analysis indicated that all expressed developing retina, but only hesr2 expression is associated spatially with gliogenesis. Each member was then misexpressed retrovirus retinal explant cultures prepared from...
In the developing mammalian basal telencephalon, neural progenitors from subpallium generate majority of inhibitory medium spiny neurons (MSNs) in striatum, while both pallial- and subpallial-derived contribute to excitatory neuronal diversity amygdala. Using a combination approaches, including genetic fate mapping, cell birth dating, migration assays, electrophysiology, we find that cells derived Emx1 lineage two distinct populations mature forebrain: MSNs striatum functionally subclasses...
ABSTRACT During forebrain development, a telencephalic organizer called the cortical hem is crucial for inducing hippocampal fate in adjacent neuroepithelium. How restricted to its medial position therefore fundamental patterning issue. Here, we demonstrate that Foxg1-Lhx2 interactions are formation of hem. Loss either gene causes region neuroepithelium transform into We show FOXG1 regulates Lhx2 expression primordium. In absence Foxg1, presence sufficient suppress fate, and markers appear...
Abstract The bimodal requisite for a genetic program and external stimuli is key feature of sensory circuit formation. However, the contribution cell-intrinsic codes to directing sensory-specific circuits remains unknown. Here, we identify earliest molecular that preselects projection neuron types in neocortex. Mechanistically, Foxg1 binds an H3K4me1-enriched enhancer site repress COUP-TFI , where ectopic acquisition layer 4 cells transforms local neurons callosal with pyramidal...
GABAergic inhibitory interneurons, originating from the embryonic ventral forebrain territories, traverse a convoluted migratory path to reach neocortex. These interneuron precursors undergo sequential phases of tangential and radial migration before settling into specific laminae during differentiation. Here, we show that developmental trajectory FoxG1 expression is dynamically controlled in these at critical junctures migration. By utilizing mouse genetic strategies, elucidate pivotal role...
Neuronal subtype specification depends on multiple transcription factors such as basic helix-loop-helix (bHLH) factors. However, factor codes for most neurons remain to be determined. Here, we report identification of a novel mouse bHLH factor, termed Heslike, that has Hes1-like domain and transcriptional repressor activity. Heslike is coexpressed with the Mash1 in brain regions give rise GABAergic neurons. In mesencephalon caudal diencephalon, coexpression initially restricted small but...