A5019341669- DNA Repair Mechanisms
- Telomeres, Telomerase, and Senescence
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- Genetics, Aging, and Longevity in Model Organisms
- RNA modifications and cancer
- Peptidase Inhibition and Analysis
- Chromosomal and Genetic Variations
- Signaling Pathways in Disease
- RNA and protein synthesis mechanisms
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Microplastics and Plastic Pollution
- Fungal and yeast genetics research
- Cancer-related gene regulation
- Endoplasmic Reticulum Stress and Disease
- Trace Elements in Health
- RNA Research and Splicing
- Bacterial Genetics and Biotechnology
- Nuclear Structure and Function
- ATP Synthase and ATPases Research
- Cellular transport and secretion
- Genetics and Neurodevelopmental Disorders
- Electrochemical sensors and biosensors
Centre de Recherche en Cancérologie de Marseille
2015-2023
La Ligue Contre le Cancer
2015-2023
Aix-Marseille Université
2009-2023
Centre National de la Recherche Scientifique
2005-2023
Inserm
2012-2023
Institut Paoli-Calmettes
2017-2023
Institut Pprime
2017
Institut de Biologie Structurale
1997-2006
Laboratoire d'Ingénierie des Systèmes Macromoléculaires
1996-2005
University of Manchester
2000-2001
The Set1 family of histone H3 lysine 4 (H3K4) methyltransferases is highly conserved from yeast to human. Here we show that the complex (Set1C) directly binds RNA in vitro through regions comprise double recognition motifs (dRRM) and N-SET domain within its subunit Spp1. To investigate functional relevance binding, performed UV crosslinking (CRAC) for polymerase II parallel with ChIP-seq experiments. nascent transcripts dRRM. binding important define appropriate topology Set1C distribution...
RPA and Pif1 cooperate to remove G-rich structures at both leading lagging strand – In Saccharomyces cerevisiae, the absence of helicase induces instability G4-containing CEB1 minisatellite during but not replication. We report that maintain stability when G4 forming is either on or templates. At strand, acts in same pathway as stability. Consistent with this result, co-precipitates Pif1. This association between affected by rfa1-D228Y mutation lowers affinity particular for single-stranded...
MINI REVIEW article Front. Oncol., 28 February 2013Sec. Cancer Molecular Targets and Therapeutics Volume 3 - 2013 | https://doi.org/10.3389/fonc.2013.00039
Abstract Histone H3 lysine 56 acetylation in Saccharomyces cerevisiae is required for the maintenance of genome stability under normal conditions and upon DNA replication stress. Here we show that absence replisome components become deleterious when forks collapse at natural block sites. This lethality not a direct consequence chromatin assembly defects during fork progression. Rather, our genetic analyses suggest presence replicative stress uncouples Cdc45–Mcm2-7–GINS helicase complex...
Abstract Ty1 mobile DNA element is the most abundant and mutagenic retrotransposon present in genome of budding yeast Saccharomyces cerevisiae. Protein regulator transposition 105 (Rtt105) associates with large subunit RPA facilitates its loading onto a single-stranded at replication forks. Here, we dissect role RTT105 maintenance stability under normal conditions upon various stresses through multiple genetic analyses. essential for viability cells experiencing problems lacking functional...
The protein import pathway that targets proteins to the mitochondrial matrix has been extensively characterized in past 15 years. Variations of this account for sorting other compartments as well, but insertion integral inner membrane lacking a presequence is mediated by distinct translocation machinery. This consists complex Tim9 and Tim10, two homologous, Zn2+-binding chaperone passage hydrophobic precursor across aqueous inter-membrane space. then targeted another, inner-membrane-bound,...
Chromatin assembly and the establishment of sister chromatid cohesion are intimately connected to progression DNA replication forks. Here we examined genetic interaction between heterotrimeric chromatin factor-1 (CAF-1), a central component during replication, core replisome Ctf4. We find that CAF-1 deficient cells as well affected in newly-synthesized H3-H4 histones deposition exhibit severe negative growth with ctf4Δ mutant. dissected role maintenance genome stability yeast cells. In...
Homologous recombination factors play a crucial role in protecting nascent DNA during replication, but the of chromatin this process is largely unknown. Here, we used bacterial Tus/Ter barrier known to induce site-specific replication fork stalling S. cerevisiae. We report that Set1C subunit Spp1 recruited behind stalled independently its interaction with Set1. recruitment depends on PHD domain H3K4me3 parental histones deposited fork. Its prevents accumulation ssDNA at by restricting access...