A5055126009- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
- Histone Deacetylase Inhibitors Research
- RNA Research and Splicing
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- PARP inhibition in cancer therapy
- DNA and Nucleic Acid Chemistry
- Chromatin Remodeling and Cancer
- Chromosomal and Genetic Variations
- Fungal and yeast genetics research
- Peptidase Inhibition and Analysis
- Toxin Mechanisms and Immunotoxins
- Fungal Infections and Studies
- Protein Degradation and Inhibitors
- Genetics, Bioinformatics, and Biomedical Research
- Acute Myeloid Leukemia Research
- Antifungal resistance and susceptibility
- Plant Molecular Biology Research
- Advanced Proteomics Techniques and Applications
- Cancer-related gene regulation
- Enzyme Structure and Function
- Microtubule and mitosis dynamics
Université de Montréal
2013-2023
Institute for Research in Immunology and Cancer
2012-2023
University of Tsukuba
2008
Cancer Research UK
2002-2005
Cold Spring Harbor Laboratory
1995-2000
University of Cambridge
1993-2000
Institut Curie
1998
Université Laval
1995
University of Guelph
1995
Centre hospitalier de l'Université Laval
1990
Heterochromatin protein 1 (HP1beta), a key component of condensed DNA, is strongly implicated in gene silencing and centromeric cohesion. has been considered static structure, stabilizing crucial aspects nuclear organization prohibiting access to transcription factors. We demonstrate here, by fluorescence recovery after photobleaching, that green fluorescent protein-HP1beta fusion highly mobile within both the euchromatin heterochromatin ex vivo resting murine T cells. Moreover, cell...
RbAp46 and RbAp48 (pRB-associated proteins p46 p48, also known as RBBP7 RBBP4, respectively) are highly homologous histone chaperones that play key roles in establishing maintaining chromatin structure. We report here the crystal structure of human bound to H4. folds into a seven-bladed β propeller binds H4 groove formed between an N-terminal α helix extended loop inserted blade six. Surprisingly, adopts different conformation when interacting with than it does either nucleosome or complex...
Methylation of histone H3 lysine 4 (H3K4me) is an evolutionarily conserved modification whose role in the regulation gene expression has been extensively studied. In contrast, function H3K4 acetylation (H3K4ac) received little attention because a lack tools to separate its from that H3K4me. Here we show that, addition being methylated, also acetylated budding yeast. Genetic studies reveal acetyltransferases (HATs) Gcn5 and Rtt109 contribute vivo. Whilst removal H3K4ac euchromatin mainly...
The subcellular distribution and posttranslational modification of human chromatin assembly factor 1 (CAF-1) have been investigated after UV irradiation HeLa cells. In an asynchronous cell population only a subfraction the two large CAF-1 subunits, p150 p60, were found to exist in chromatin-associated fraction. This fraction is most abundant during S phase nonirradiated cells much reduced G2 After irradiation, form dramatically increased all irrespective their position cycle. Such...
Histone deacetylase inhibitors (HDACi) represent a promising avenue for cancer therapy. We applied mass spectrometry (MS) to determine the impact of clinically relevant HDACi on global levels histone acetylation. Intact profiling revealed that SAHA and MS-275 globally increased H3 H4 acetylation in both normal diploid fibroblasts transformed human cells. lysine 56 (H3K56ac) recently elicited much interest controversy due its potential as diagnostic prognostic marker broad diversity cancers....
In Saccharomyces cerevisiae, histone H3 lysine 56 acetylation (H3K56ac) occurs in newly synthesized histones that are deposited throughout the genome during DNA replication. Defects H3K56ac sensitize cells to genotoxic agents, suggesting this modification plays an important role damage response. However, links between acetylation, nascent chromatin structure, and response poorly understood. Here we report devoid of sensitive sustained transient exposure methyl methanesulfonate (MMS) or...
Human transcriptional coactivator PC4 is a highly abundant multifunctional protein which plays diverse important roles in cellular processes, including transcription, replication, and repair. It also unique activator of p53 function. Here we report that bona fide component chromatin with distinct organization ability. predominantly associated the throughout stages cell cycle broadly distributed on mitotic chromosome arms punctate manner except for centromere. selectively interacts core...
Chromatin assembly factor 1 (CAF-1) deposits histones H3 and H4 rapidly behind replication forks through an interaction with the proliferating cell nuclear antigen (PCNA), a DNA polymerase processivity that also binds to number of enzymes other proteins act on nascent DNA. The mechanisms enable CAF-1 PCNA-binding function harmoniously at fork are poorly understood. Here we report large subunit human (p150) contains two distinct PCNA peptides (PIPs). N-terminal PIP strongly in vitro but,...
In Saccharomyces cerevisiae, acetylation of lysine 56 (Lys-56) in the globular domain histone H3 plays an important role response to genotoxic agents that interfere with DNA replication. However, regulation and biological function this modification are poorly defined other eukaryotes. Here we show Lys-56 Schizosaccharomyces pombe occurs transiently during passage through S-phase is normally removed G(2). Genotoxic cause double strand breaks replication elicit a delay deacetylation Lys-56....