A5089372534- Microtubule and mitosis dynamics
- Multiple Sclerosis Research Studies
- Photosynthetic Processes and Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Cerebral Palsy and Movement Disorders
- Genomics and Chromatin Dynamics
- Genetic Neurodegenerative Diseases
- Advanced Fluorescence Microscopy Techniques
- Stroke Rehabilitation and Recovery
- Peripheral Neuropathies and Disorders
- Cellular transport and secretion
- Cell Image Analysis Techniques
- Muscle activation and electromyography studies
- Cellular Mechanics and Interactions
- DNA Repair Mechanisms
- Chromosomal and Genetic Variations
- Brain Tumor Detection and Classification
- Plant nutrient uptake and metabolism
- Reproductive Biology and Fertility
- Rheumatoid Arthritis Research and Therapies
- Sleep and related disorders
- Spaceflight effects on biology
- Data Visualization and Analytics
- Nuclear Structure and Function
- Botulinum Toxin and Related Neurological Disorders
Novartis (Switzerland)
2014-2023
Roche (Switzerland)
2022-2023
University of California, San Diego
2019
Cedars-Sinai Smidt Heart Institute
2019
Microsoft Research (United Kingdom)
2018
Université de Montréal
2008-2016
Institute for Research in Immunology and Cancer
2008-2016
Marine Biological Laboratory
2010
Scripps Research Institute
2005-2010
Harvard University
2007
During mitosis in most eukaryotic cells, chromosomes align and form a metaphase plate halfway between the spindle poles, about which they exhibit oscillatory movement. These movements are accompanied by changes distance sister kinetochores, commonly referred to as breathing. We developed live cell imaging assay combined with computational image analysis quantify properties dynamics of kinetochores three dimensions. show that baseline oscillation breathing speeds late prometaphase set...
Background Wearable devices are valuable assessment tools for patient outcomes in contexts such as clinical trials. To be successfully deployed, however, participants must willing to wear them. Another concern is that usability studies rarely published, often fail test beyond 24 hours, and need repeated frequently ensure contemporary assessed. Objective This study aimed compare multiple wearable sensors a real-world context establish their within an older adult (>50 years) population....
The discovery that drugs targeting a single G protein-coupled receptor (GPCR) can differentially modulate distinct subsets of the signaling repertoire has created challenge for drug at these important therapeutic targets. Here, we demonstrate label-free assay based on cellular impedance provides real-time integration multiple events engaged upon GPCR activation. Stimulation β2-adrenergic (β2AR) in living cells with prototypical agonist isoproterenol generated complex, multi-featured response...
The cortical mechanisms that drive the series of mitotic cell shape transformations remain elusive. In this paper, we identify two novel networks collectively control dynamic reorganization cortex. We demonstrate Moesin, an actin/membrane linker, integrates these to synergize forces transformations. find Pp1-87B phosphatase restricts high Moesin activity early mitosis and down-regulates at polar cortex, after anaphase onset. Overactivation cortex impairs elongation thus cytokinesis, whereas...
In Saccharomyces cerevisiae, histone H3 lysine 56 acetylation (H3K56ac) occurs in newly synthesized histones that are deposited throughout the genome during DNA replication. Defects H3K56ac sensitize cells to genotoxic agents, suggesting this modification plays an important role damage response. However, links between acetylation, nascent chromatin structure, and response poorly understood. Here we report devoid of sensitive sustained transient exposure methyl methanesulfonate (MMS) or...
Membrane trafficking has well-defined roles during cell migration. However, its regulation is poorly characterized. In this paper, we describe the first screen for putative Rab–GTPase-activating proteins (GAPs) collective migration of Drosophila melanogaster border cells (BCs), identify uncharacterized protein Evi5 as an essential membrane regulator, and molecular mechanism by which regulates BC requires Rab-GAP activity to fulfill functions acts a GAP Rab11. Both loss gain function blocked...
The black-and-white patterning of tendon fascicles when visualized by light microscopy, also known as crimp, is a well-known feature fiber-forming collagens. However, not much about its development, function and response to strain. objective this study investigate the interaction tenocyte crimp morphology well their changes with increasing age acute In contrast previous studies, which used indirect measures, such polarized light, structure, visualizes internal structure in three dimensions...
Multicellular development requires that cells reduce in size as a result of consecutive cell divisions without increase embryo volume. To maintain cellular integrity, organelle adapts to throughout development. During mitosis, the longest chromosome arm must be shorter than half mitotic spindle for proper segregation. Using high-resolution time-lapse microscopy living Caenorhabditis elegans embryos, we have quantified relation between and length. In control length scaled size. Artificial...
During cytokinesis, the cell undergoes a dramatic shape change as it divides into two daughter cells. Cell changes in cytokinesis are driven by cortical ring rich actin filaments and nonmuscle myosin II. The closes via actomyosin contraction coupled with depolymerization. Of interest, closure hence furrow ingression nonconcentric (asymmetric) within division plane across Metazoa. This nonconcentricity can occur persist even without preexisting asymmetric cues, such spindle placement or...
INTRODUCTION The development of cloning vectors for green fluorescent protein (GFP) and the simplicity yeast reverse genetics allow straightforward labeling proteins in living cells. Budding fission are therefore attractive organisms which to study dynamic cellular processes such as growth, cell division, morphogenesis using live fluorescence microscopy. This article focuses on methods culture, mount, observe budding cells three-dimensional (3D) microscopy, but broadly applicable other types...
Summary We present an algorithm for the three‐dimensional (3D) tracking of multiple fluorescent subresolution tags with super‐resolution in images living cells. Recently, we described automatic detection such single frames and demonstrated its potential a biological system. The presented here adds to tag detector module relative signals between frames. As detection, main problem arises when interfere. propose novel multitemplate matching framework that exploits knowledge microscope point...
Processing bodies (PBs, or P bodies) are cytoplasmic granules involved in mRNA storage and degradation that participate the regulation of gene expression. PBs concentrate nontranslated mRNAs several factors decay translational repression, including eukaryotic translation initiation factor 4E-transporter (4E-T). 4E-T is required for PB assembly, but little known about molecular mechanisms regulate its function. Here, we demonstrate oxidative stress promotes multisite phosphorylation. We show...