A5061850579- Microtubule and mitosis dynamics
- Nuclear Structure and Function
- Herpesvirus Infections and Treatments
- Genomics and Chromatin Dynamics
- Mitochondrial Function and Pathology
- Virus-based gene therapy research
- Ubiquitin and proteasome pathways
- Plant Molecular Biology Research
- Animal Virus Infections Studies
- Cancer-related Molecular Pathways
- Protein Degradation and Inhibitors
- Genetics and Neurodevelopmental Disorders
- Microfluidic and Bio-sensing Technologies
- Advanced Proteomics Techniques and Applications
- Neonatal Respiratory Health Research
- Orbital Angular Momentum in Optics
- Pregnancy and preeclampsia studies
- Reproductive System and Pregnancy
- Wireless Body Area Networks
- Bioinformatics and Genomic Networks
- Plant nutrient uptake and metabolism
- Microfluidic and Capillary Electrophoresis Applications
- Cancer, Lipids, and Metabolism
- Transgenic Plants and Applications
- Viral Infectious Diseases and Gene Expression in Insects
University of Dundee
2004-2023
Google (United States)
2017
Marine Biological Laboratory
2010
Wellcome Trust
2010
During mitosis in most eukaryotic cells, chromosomes align and form a metaphase plate halfway between the spindle poles, about which they exhibit oscillatory movement. These movements are accompanied by changes distance sister kinetochores, commonly referred to as breathing. We developed live cell imaging assay combined with computational image analysis quantify properties dynamics of kinetochores three dimensions. show that baseline oscillation breathing speeds late prometaphase set...
Mitotic entry and progression require the activation of several mitotic kinases proper regulation localization phosphatases. The activity each these enzymes is tightly controlled through a series specific activators, inhibitors regulatory subunits. Two proteins, Ensa Arpp-19, were recently identified as PP2A-B55 are critical for allowing full Cdk1/cyclin B into mitosis. Here we show that Bod1, protein required chromosome alignment at mitosis, shares sequence similarity with Arpp-19...
We have combined the proteomic analysis of Xenopus laevis in vitro–assembled chromosomes with RNA interference and live cell imaging HeLa cells to identify novel factors required for proper chromosome segregation. The first these is Bod1, a protein conserved throughout metazoans that associates large macromolecular complex localizes kinetochores spindle poles during mitosis. Small interfering depletion Bod1 produces elongated mitotic spindles severe biorientation defects. Bod1-depleted form...
Here we report a stop-mutation in the BOD1 (Biorientation Defective 1) gene, which co-segregates with intellectual disability large consanguineous family, where individuals that are homozygous for mutation have no detectable mRNA or protein. The protein is required proper chromosome segregation, regulating phosphorylation of PLK1 substrates by modulating Protein Phosphatase 2A (PP2A) activity during mitosis. We fibroblast cell lines derived from carriers show aberrant localisation cycle...
BACKGROUND: Quantitative proteomic analyses have traditionally used two-dimensional gel electrophoresis (2DE) for separation and characterisation of complex protein mixtures. Among the difficulties associated with this approach is solubilisation mixtures isoelectric focusing (IEF). To find optimal formulation multi-component IEF rehydration buffer (RB) we applied Taguchi method, a widely robust optimisation industrial processes, to determine concentrations detergents, carrier ampholytes...
Although recently developed placenta-on-chip systems opened promising perspectives for placental barrier modeling, they still lack physiologically relevant trophoblasts and are poorly amenable to high-throughput studies. We aimed implement human-induced pluripotent stem cells (hiPSC)-derived into a multi-well microfluidic device develop scalable model. When cultured in perfused micro-channel against collagen-based matrix, hiPSC-derived self-arranged 3D structure showing invasive behavior,...
Open reading frame UL12 of herpes simplex virus type 1 (HSV-1) encodes an alkaline nuclease that has previously been implicated in processing the complex, branched, viral DNA replication intermediates and allowing egress DNA-containing capsids from nucleus. This report describes experiments using HSV-1 null mutant ambUL12, which aim to explain approximately 200- 1000-fold decrease yield infectious virus, compared with wild-type (wt) HSV-1, non-complementing cells. A detailed examination...
Regulation of protein phosphatase activity by endogenous inhibitors is an important mechanism to control phosphorylation in cells. We recently identified Biorientation defective 1 (Bod1) as a small inhibitor 2A containing the B56 regulatory subunit (PP2A-B56). This controls amount several kinetochore proteins and thus establishment load-bearing chromosome-spindle attachments time for accurate separation sister chromatids mitosis. Like PP2A-B56, Bod1 directly localizes mitotic kinetochores...
The alkaline nuclease (AN) encoded by gene UL12 of herpes simplex virus type 1 (HSV-1) is essential for efficient replication but its role during the lytic cycle remains incompletely understood. Inactivation results in reductions viral DNA synthesis, packaging, egress DNA-containing capsids from nucleus and ability progeny virions to initiate new cycles infection. Mechanistically, AN has been implicated resolving branched structures HSV-1 replicative intermediates prior encapsidation,...
Abstract Regulation of protein phosphatase activity by endogenous inhibitors is an important mechanism to control phosphorylation in cells. We recently identified Biorientation defective 1 (Bod1) as a small inhibitor PP2A-B56. This controls the amount mitotic kinetochore proteins and thus establishment load-bearing chromosome-spindle attachments time for accurate separation sister chromatids mitosis. The PP2A-B56 regulatory Bod1 directly localises kinetochores required correct segregation...
Although recently developed placenta-on-chip systems opened promising perspectivesfor placental barrier modelling, they still lack physiologically relevant trophoblasts andare poorly amenable to high-throughput studies. We aimed implement humaninduced pluripotent stem cells (hiPSC)-derived into a multi-wellmicrofluidic device develop and scalable barriermodel. When cultured in perfused micro-channel against collagen-based matrix,hiPSC-derived self-arranged 3D structure showing...