A5068974961- Pregnancy and preeclampsia studies
- Nitric Oxide and Endothelin Effects
- Redox biology and oxidative stress
- S100 Proteins and Annexins
- Single-cell and spatial transcriptomics
- Molecular Biology Techniques and Applications
- Pluripotent Stem Cells Research
- Renin-Angiotensin System Studies
- Gene expression and cancer classification
- Sulfur Compounds in Biology
- Cardiovascular Issues in Pregnancy
- Prenatal Screening and Diagnostics
- Reproductive System and Pregnancy
- Neonatal Respiratory Health Research
- RNA and protein synthesis mechanisms
- Birth, Development, and Health
- Art Therapy and Mental Health
- 3D Printing in Biomedical Research
- Blood Coagulation and Thrombosis Mechanisms
- Pregnancy and Medication Impact
- Protein Structure and Dynamics
- Endometriosis Research and Treatment
- Tissue Engineering and Regenerative Medicine
- Pulmonary Hypertension Research and Treatments
- Heat shock proteins research
University of Dundee
2019-2024
Ninewells Hospital
2021
Institut Supérieur des Biotechnologies de Paris
2018
Although recently developed placenta-on-chip systems opened promising perspectives for placental barrier modeling, they still lack physiologically relevant trophoblasts and are poorly amenable to high-throughput studies. We aimed implement human-induced pluripotent stem cells (hiPSC)-derived into a multi-well microfluidic device develop scalable model. When cultured in perfused micro-channel against collagen-based matrix, hiPSC-derived self-arranged 3D structure showing invasive behavior,...
Organ-on-a-chip technology incorporating stem cell techniques represents a promising strategy to improve modeling of human organs. Here, we present protocol for generating standardized 3D placenta-on-a-chip model using trophoblast derived from induced pluripotent cells (hiPSCs). We describe steps seeding hiPSCs into multi-chip OrganoPlate devices and on-chip differentiation trophoblasts against an extracellular matrix under perfused conditions. then detail procedures conducting functional...
<h3></h3> The placenta is a unique cardiovascular organ that receives blood supplies from both the maternal and fetal system. Dysfunction of can lead to gestational hypertension lifelong consequences for mother baby. Yet our ability study dysfunction often relies on sourcing trophoblasts either term-placenta or cell lines. We aimed validate 3D placenta-on-a-chip model be used in high-throughput system using human induced pluripotent stems cells (HiPSC) derived-trophoblast. <h3>Methods</h3>...
<h3></h3> Jagged1 has essential roles in cardiovascular development and maintenance by regulating angiogenesis, which is tightly controlled redox signalling endothelial cells (EC). Proteins sense signals through certain cysteine residues forming oxidative post translational modifications (ox-PTM) alter protein function. Iodoacetyl-tandem mass tag (Iodo-TMT) proteomics was performed to identify the range of redox-sensitive proteins involved angiogenesis. To induce changes we used...
<h3>Background</h3> Preeclampsia is a severe pregnancy complication which has long-term consequences on mother and child. Elevation of the soluble isoform anti-angiogenic factor sFlt1 in maternal circulation pivotal development gestational hypertension, kidney placental dysfunction. Hallmarks preeclampsia include oxidative stress, endothelial S-glutathionylation, post-translational modification (oxPTM) reversed by glutaredoxin (Glrx), emerging as an important regulator angiogenic signaling...
Abstract Here we develop a temperature sensitive expression vector that allows the selective production of distinct proteins over different ranges with single plasmid. We use E. coli cold shock translational control system (the cspA 5’Untranslated Transcribed Region - UTR) to drive desired protein below certain threshold, and lambda phage pL/cI857 transcriptional repressor above threshold. In this developmental work chromogenic reporter amilCP (blue chromoprotein) monomeric red fluorescent...
Although recently developed placenta-on-chip systems opened promising perspectivesfor placental barrier modelling, they still lack physiologically relevant trophoblasts andare poorly amenable to high-throughput studies. We aimed implement humaninduced pluripotent stem cells (hiPSC)-derived into a multi-wellmicrofluidic device develop and scalable barriermodel. When cultured in perfused micro-channel against collagen-based matrix,hiPSC-derived self-arranged 3D structure showing...
<h3></h3> Elevation of oxidative stress is explicitly linked to hypertensive pregnancies with high levels measurable in the maternal circulation. Hypertensive lead life-long risk cardiac dysfunction, yet underpinning molecular pathway are unknown. Oxidative modify proteins via post-translational modifications (ox-PTMs) which can regulate intracellular signalling by either potentiating or inhibiting protein activity. One these, S-glutathionylation a common oxPTM reversed glutaredoxin (Glrx)....
<h3>Background</h3> Elevation of the anti-angiogenic factor soluble Flt-1 (sFlt-1) is pivotal in development preeclampsia phenotype hypertension and kidney dysfunction during pregnancy. Likewise, oxidative stress explicitly linked to with high levels measurable both placenta maternal circulation. Yet antioxidant therapy has failed, some cases worsening pregnancy outcomes. The underlying molecular mechanisms affected by redox signalling remain unclear. Reactive oxygen/nitrogen species (RONS)...
Abstract Background Elevation of circulating anti-angiogenic factors is pivotal in the development preeclampsia (PE) phenotype incomplete vascular remodelling, hypertension and kidney dysfunction during pregnancy. Oxidative stress explicitly linked to PE with high levels measurable placenta. Yet antioxidant therapy has failed, some cases worsening pregnancy outcomes. The modulation protein activity by reversible oxidative post-translational modifications (oxPTM) under low reactive oxygen...
Abstract Redox signalling plays an important role in endothelial cell (EC) physiology and pathophysiology. Proteins sense redox signals via cysteine thiol groups. A common oxidative post-translational modification (oxPTM) on thiols is S-glutathionylation which reversed to a free state by glutaredoxin (Glrx). OxPTMs alter protein function, location stability. Identifying proteins undergo will help determine the of EC function. proteome-wide screen human cardiac microvascular cells identified...