A5077272209- Pregnancy and preeclampsia studies
- 3D Printing in Biomedical Research
- Neonatal Respiratory Health Research
- Nitric Oxide and Endothelin Effects
- Wireless Body Area Networks
- Pluripotent Stem Cells Research
- Endometriosis Research and Treatment
- Neuroscience and Neural Engineering
- Intensive Care Unit Cognitive Disorders
- Pulmonary Hypertension Research and Treatments
- MicroRNA in disease regulation
- Molecular Communication and Nanonetworks
- Sulfur Compounds in Biology
- Reproductive System and Pregnancy
- Anesthesia and Neurotoxicity Research
- Cardiovascular Issues in Pregnancy
- Neonatal and fetal brain pathology
- Acute Ischemic Stroke Management
- Prenatal Screening and Diagnostics
- Tissue Engineering and Regenerative Medicine
- Electrolyte and hormonal disorders
- Barrier Structure and Function Studies
- Ion channel regulation and function
- Lipid Membrane Structure and Behavior
- Receptor Mechanisms and Signaling
Mimetas (Netherlands)
2021-2024
Maastricht University
2021-2023
In ischemic stroke, the function of cerebral vasculature is impaired. This vascular structure formed by so-called neurovascular unit (NVU). A better understanding mechanisms involved in NVU dysfunction and recovery may lead to new insights for development highly sought therapeutic approaches. To date, there remains an unmet need complex human vitro models study events seen brain.We here describe a on-a-chip model using platform that allows culture 40 chips parallel. The comprises perfused...
Pathologies associated with uteroplacental hypoxia, such as preeclampsia are among the leading causes of maternal and perinatal morbidity in world. Its fundamental mechanisms yet poorly understood due to a lack good experimental models. Here we report an vitro model placental barrier, based on co-culture trophoblasts endothelial cells against collagen extracellular matrix microfluidic platform. The yields functional syncytium barrier properties, polarization, secretion relevant membrane...
Traditionally, to quantify permeability of a biological barrier, the initial slope is used, based on assumption sink condition (concentration donor constant, and receiver increases less than 10%). With on-a-chip barrier models, this fails in cell-free or leaky conditions, which requires use exact solution. To encounter time delay from performing assay acquiring data, we present protocol with equation modified incorporate offset.
Although recently developed placenta-on-chip systems opened promising perspectives for placental barrier modeling, they still lack physiologically relevant trophoblasts and are poorly amenable to high-throughput studies. We aimed implement human-induced pluripotent stem cells (hiPSC)-derived into a multi-well microfluidic device develop scalable model. When cultured in perfused micro-channel against collagen-based matrix, hiPSC-derived self-arranged 3D structure showing invasive behavior,...
Reactive oxygen species (ROS) have different properties and biological functions. They contribute to cell signaling and, in excessive amounts, oxidative stress (OS). Although ROS is pivotal a wide number of physiological systems pathophysiological processes, direct quantification vivo quite challenging mainly limited vitro studies. Even though advanced culture techniques, like on-a-chip culture, overcome the lack crucial vivo-like aspects 2D majority studies are generally performed 2D. Here...
Organ-on-a-chip technology incorporating stem cell techniques represents a promising strategy to improve modeling of human organs. Here, we present protocol for generating standardized 3D placenta-on-a-chip model using trophoblast derived from induced pluripotent cells (hiPSCs). We describe steps seeding hiPSCs into multi-chip OrganoPlate devices and on-chip differentiation trophoblasts against an extracellular matrix under perfused conditions. then detail procedures conducting functional...
<h3></h3> The placenta is a unique cardiovascular organ that receives blood supplies from both the maternal and fetal system. Dysfunction of can lead to gestational hypertension lifelong consequences for mother baby. Yet our ability study dysfunction often relies on sourcing trophoblasts either term-placenta or cell lines. We aimed validate 3D placenta-on-a-chip model be used in high-throughput system using human induced pluripotent stems cells (HiPSC) derived-trophoblast. <h3>Methods</h3>...
Although recently developed placenta-on-chip systems opened promising perspectivesfor placental barrier modelling, they still lack physiologically relevant trophoblasts andare poorly amenable to high-throughput studies. We aimed implement humaninduced pluripotent stem cells (hiPSC)-derived into a multi-wellmicrofluidic device develop and scalable barriermodel. When cultured in perfused micro-channel against collagen-based matrix,hiPSC-derived self-arranged 3D structure showing...
Abstract Background Elevation of circulating anti-angiogenic factors is pivotal in the development preeclampsia (PE) phenotype incomplete vascular remodelling, hypertension and kidney dysfunction during pregnancy. Oxidative stress explicitly linked to PE with high levels measurable placenta. Yet antioxidant therapy has failed, some cases worsening pregnancy outcomes. The modulation protein activity by reversible oxidative post-translational modifications (oxPTM) under low reactive oxygen...