A5052579243- Ubiquitin and proteasome pathways
- Signaling Pathways in Disease
- Peptidase Inhibition and Analysis
- Cellular transport and secretion
- Cellular Mechanics and Interactions
- Hippo pathway signaling and YAP/TAZ
- Lymphoma Diagnosis and Treatment
- Autophagy in Disease and Therapy
- Protein Kinase Regulation and GTPase Signaling
- PI3K/AKT/mTOR signaling in cancer
- Cancer-related gene regulation
- Atherosclerosis and Cardiovascular Diseases
- Eosinophilic Disorders and Syndromes
- Axon Guidance and Neuronal Signaling
- Phagocytosis and Immune Regulation
- T-cell and Retrovirus Studies
- Microtubule and mitosis dynamics
- Genetic and Kidney Cyst Diseases
- Lipid Membrane Structure and Behavior
- RNA and protein synthesis mechanisms
- Neurobiology and Insect Physiology Research
- Marine Biology and Environmental Chemistry
- Biomarkers in Disease Mechanisms
- Retinal Development and Disorders
- Plant Reproductive Biology
Inserm
2009-2024
Institut des Maladies Métaboliques et Cardiovasculaires
2014-2024
Université Toulouse III - Paul Sabatier
2010-2024
Cancéropôle Île-de-France
2023
Université de Toulouse
2011-2020
Centre National de la Recherche Scientifique
2016-2020
Laboratoire de Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération
2016-2020
Institute for Research in Immunology and Cancer
2010-2013
Université de Montréal
2010-2013
Centre Hospitalier Universitaire de Toulouse
2011
Shigella keeps infected cells alive by preventing the lysosomal trafficking and degradation of an activated growth factor receptor.
Border cell migration is a stereotyped occurring during the development of Drosophila egg chamber. During this process, cluster composed six to eight follicle cells migrates between nurse toward oocyte. Receptor tyrosine kinases (RTKs) are enriched at leading edge and establish directionality their migration. Endocytosis has been shown play role in maintenance polarization; however, mechanisms involved largely unknown. In study, we show that border requires function small GTPases Rab5 Rab11...
Abstract Cells subjected to stress situations mobilize specific membranes and proteins initiate autophagy. Phosphatidylinositol-3-phosphate (PI3P), a crucial lipid in membrane dynamics, is known be essential this context. In addition nutriments deprivation, autophagy also triggered by fluid-flow induced shear epithelial cells, autophagic response depends on primary cilium (PC) signaling leads cell size regulation. Here we report that PI3KC2α, required for ciliogenesis PC functions, promotes...
Membrane trafficking has well-defined roles during cell migration. However, its regulation is poorly characterized. In this paper, we describe the first screen for putative Rab–GTPase-activating proteins (GAPs) collective migration of Drosophila melanogaster border cells (BCs), identify uncharacterized protein Evi5 as an essential membrane regulator, and molecular mechanism by which regulates BC requires Rab-GAP activity to fulfill functions acts a GAP Rab11. Both loss gain function blocked...
Diabetic patients are particularly susceptible to cardiovascular complications following atherosclerosis. Uncontrolled glycemia leads arterial dysfunction, potentially responsible for these increased risks. While the impact of hyperglycemia on endothelial cells and macrophages is well documented, its role in smooth muscle (SMCs) remains underexplored. In vivo, using a wire injury-based accelerated atherosclerosis mouse model, we showed that lipid loading SMCs. vitro, further confirmed this...
Guided cell migration is a key mechanism for positioning in morphogenesis. The current model suggests that the spatially controlled activation of receptor tyrosine kinases (RTKs) by guidance cues would limit Rac activity at leading edge, which critical establishing and maintaining polarized protrusions front. However, little known about mechanisms RTKs control local Rac. Here, using multidisciplinary approach, we identify GTP exchange factor (GEF) vav as regulator downstream developmentally...
Defects in efficient endothelial healing have been associated with complication of atherosclerosis such as post-angioplasty neoatherosclerosis and plaque erosion leading to thrombus formation. However, current preventive strategies do not consider re-endothelialization their design. Here, we investigate mechanisms linking immune processes defect re-endothelialization. We especially evaluate if targeting phosphoinositide 3-kinase γ could restore identify mediators responsible for these...
Many anaplastic large cell lymphomas (ALCL) express the chimeric oncogene NPM-ALK, which drives malignant transformation and invasion. In this study, we show that NPM-ALK expression increases matrix metalloproteinase-9 (MMP-9) expression. Accordingly, found 100% of a panel ALK(+) ALCL biopsies examined were also MMP-9(+), in contrast to only 36.3% ALK(-) tumors. Mechanistic studies revealed Rac1 drove MMP-9 secretion. The MMP inhibitor GM6001 blocking antibodies abolished invasiveness...
Border cell migration during Drosophila oogenesis is a potent model to study collective migration, process involved in development and metastasis. clusters adopt two main types of behaviour migration: linear rotational. However, the molecular mechanism controlling switch from one other unknown. Here, we demonstrate that non-muscle Myosin II (NMII, also known as Spaghetti squash) activity controls linear-to-rotational switch. Furthermore, show regulation NMII takes place downstream guidance...
Collective cell migration is central to many developmental and pathological processes. However, the mechanisms that keep collectives together coordinate movement of multiple cells are poorly understood. Using Drosophila border model, we find Protein phosphatase 1 (Pp1) activity controls collective cohesion migration. Inhibition Pp1 causes round up, dissociate, move as single with altered motility. We present evidence promotes proper levels cadherin-catenin complex proteins at cell-cell...
NPM-ALK is a chimeric tyrosine kinase detected in most anaplastic large cell lymphomas that results from the reciprocal translocation t(2,5)(p23;q35) fuses N-terminal domain of nucleophosmin (NPM) to catalytic lymphoma (ALK) receptor. The constitutive activity responsible for its oncogenicity through stimulation several downstream signaling pathways, leading proliferation, migration, and survival. We demonstrated previously high level phosphatidylinositol 5-phosphate measured...
Cyclic adenosine 3′,5′-monophosphate (cAMP)–elevating agents, such as β 2 -adrenergic receptor (β -AR) agonists and phosphodiesterase (PDE) inhibitors, remain a mainstay in the treatment of obstructive respiratory diseases, conditions characterized by airway constriction, inflammation, mucus hypersecretion. However, their clinical use is limited unwanted side effects because unrestricted cAMP elevation airways distant organs. Here, we identified A-kinase anchoring protein phosphoinositide...
Phosphoinositides are minor constituents of cell membranes playing a critical role in the regulation many cellular functions. Recent discoveries indicate that mutations several phosphoinositide kinases and phosphatases generate imbalances levels phosphoinositides, thereby leading to development human diseases. Although roles 3-kinase products PtdIns(4,5)P2 were largely studied these last years, potential phosphatidylinositol monophosphates as direct signalling molecules is just emerging....
Arterial remodeling in hypertension and intimal hyperplasia involves inflammation disrupted flow, both of which contribute to smooth muscle cell dedifferentiation proliferation. In this context, our previous results identified phosphoinositide 3-kinase γ (PI3Kγ) as an essential factor inflammatory processes the arterial wall. Here, we identify for first time a kinase-independent role nonhematopoietic PI3Kγ vascular wall during using PI3Kγ-deleted mice expressing kinase-dead version enzyme....
Cell migration is an important process involved in developmental events and pathologies such as cancer. can be classified into two types: individual collective cell movements. Compared with migration, less understood has drawn increasing attention lately because of its emerging role cancer spreading. We have recently established that Rab11 absolutely required for spatial control Rac1 activity through the cell-cell communication during movements (Ramel, et al. 2013). Moreover, we demonstrated...
Atherosclerosis is the primary cause of cardiovascular disease. The development plaque complications, such as calcification and neo-angiogenesis, strongly impacts stability a good predictor mortality in patients with atherosclerosis. Despite well-known risk factors diabetes mellitus chronic kidney disease, mechanisms involved are not fully understood. We others have identified that concentration circulating leucine-rich α-2 glycoprotein 1 (LRG1) was increased diabetic disease patients. Using...