A5046001992- Semiconductor materials and devices
- Silicon and Solar Cell Technologies
- Microtubule and mitosis dynamics
- solar cell performance optimization
- Cellular Mechanics and Interactions
- Plasmonic and Surface Plasmon Research
- Optical Coatings and Gratings
- Block Copolymer Self-Assembly
- Mycorrhizal Fungi and Plant Interactions
- Chalcogenide Semiconductor Thin Films
- Advancements in Semiconductor Devices and Circuit Design
- Molecular Junctions and Nanostructures
- Silicon Nanostructures and Photoluminescence
- Advanced Memory and Neural Computing
- Integrated Circuits and Semiconductor Failure Analysis
- Nanofabrication and Lithography Techniques
- Photonic Crystals and Applications
- Thin-Film Transistor Technologies
- Advanced Surface Polishing Techniques
- Near-Field Optical Microscopy
- Force Microscopy Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Fungal Biology and Applications
- Advanced Polymer Synthesis and Characterization
- Forest Ecology and Biodiversity Studies
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2008-2023
Aix-Marseille Université
2021-2023
CEA Grenoble
2008-2023
CEA LETI
2009-2023
Centre National de la Recherche Scientifique
2002-2023
Université Grenoble Alpes
2008-2023
Institut polytechnique de Grenoble
2008-2023
Laboratoire des Technologies de la Microélectronique
2011-2023
Morphogénie Logiciels (France)
2019-2021
Université Toulouse III - Paul Sabatier
2004-2013
Abstract Background Multicellular tumor spheroids are models of increasing interest for cancer and cell biology studies. They allow considering cellular interactions in exploring cycle division mechanisms. However, 3D imaging living is technically challenging has never been reported. Results Here, we report a major breakthrough based on the engineering multicellular expressing an histone H2B fluorescent nuclear reporter protein, specifically designed sample holders to monitor live dynamics...
Epithelial-mesenchymal transition (EMT) is an essential process both in physiological and pathological contexts. Intriguingly, EMT often associated with tissue invagination during development; however, the impact of on remodeling remain unexplored. Here, we show that at initiation process, cells produce apico-basal force, orthogonal to surface epithelium, constitutes important driving force for Drosophila. When ectopically induced, starting their delamination generate induce ectopic folding....
Transmission spectra of metallic films or membranes perforated by arrays subwavelength slits holes have been widely interpreted as resonance absorption surface plasmon polaritons. Alternative interpretations involving evanescent waves diffracted on the also proposed. These two approaches lead to divergent predictions for some wave properties. Using far-field interferometry, we carried out a series measurements elementary one-dimensional structures with aim testing key properties and...
In fission yeast, erroneous attachments of spindle microtubules to kinetochores are frequent in early mitosis. Most corrected before anaphase onset by a mechanism involving the protein kinase Aurora B, which destabilizes kinetochore (ktMTs) absence tension between sister chromatids. this paper, we describe minimal mathematical model yeast chromosome segregation based on stochastic attachment and detachment ktMTs. The accurately reproduces timing correct biorientation seen yeast. Prevention...
High density self-assembled platinum nanodots are elaborated using a radio frequence sputtering technique and embedded in memory structures. Electronic microscopy methods used to characterize the morphology. Scanning electron scanning transmission observations allow quantification of (>3×1012cm−2) size (2–3nm) nanocrystals, whereas their crystallinity is investigated high-resolution microscopy. Then, capacitance-voltage sweep measurements give excellent characteristics with 7.1V...
Epithelial sheets undergo highly reproducible remodeling to shape organs. This stereotyped morphogenesis depends on a well-defined sequence of events leading the regionalized expression developmental patterning genes that finally triggers downstream mechanical forces drive tissue at pre-defined position. However, how mechanics controls morphogenetic robustness when challenged by intrinsic perturbations in close proximity has never been addressed. Using Drosophila developing leg, we show bias...
Faithful segregation of sister chromatids requires the attachment each kinetochore (Kt) to microtubules (MTs) that extend from opposite spindle poles. Merotelic Kt orientation is a Kt–MT misattachment in which single binds MTs both poles rather than just one. Genetic induction merotelic during anaphase fission yeast resulted intra-Kt stretching followed by either correction or disruption. Laser ablation revealed and were dependent on MT forces. The presence multiple chromosomes linearly...
A detailed study of the self-assembly and coverage by 1-nonanethiol sputtered Au surfaces using molecular resolution atomic force microscopy (AFM) scanning tunneling (STM) is presented. The monolayer self-assembles on a smooth surface composed predominantly {111} oriented grains. domains alkanethiol are observed with sizes typically 5−25 nm, multiple can exist within one grain. STM imaging shows that (4 × 2) superlattice structure as (3 2 ) when imaged under noncontact AFM conditions....
In eukaryotic cells, proper formation of the spindle is necessary for successful cell division. We have studied chromosome recapture in fission yeast Schizosaccharomyces pombe. show by live analysis that lost kinetochores interact laterally with intranuclear microtubules (INMs) and both microtubule depolymerization (end-on pulling) minus-end-directed movement (microtubule sliding) contribute to retrieval pole body (SPB). find motor Klp2 colocalizes kinetochore during its transport SPB...
In higher eukaryotes, efficient chromosome congression relies, among other players, on the activity of chromokinesins. Here, we provide a quantitative analysis kinetochore oscillations and positioning in S. Pombe, model organism lacking wild type cells, chromosomes align during prophase while oscillating, maintain this alignment throughout metaphase. Chromosome are dispensable both for stable Kinesin-8 controls by regulating their oscillations. Oppositely, demonstrate that fission yeast an...
Border cell migration during Drosophila oogenesis is a potent model to study collective migration, process involved in development and metastasis. clusters adopt two main types of behaviour migration: linear rotational. However, the molecular mechanism controlling switch from one other unknown. Here, we demonstrate that non-muscle Myosin II (NMII, also known as Spaghetti squash) activity controls linear-to-rotational switch. Furthermore, show regulation NMII takes place downstream guidance...
A generic, CMOS compatible strategy for transferring a block copolymer template to semiconductor substrate is demonstrated. An aluminum oxide (Al(2)O(3)) hard mask selectively deposited by atomic layer deposition in an organized array of holes obtained PS matrix via PS-b-PMMA self-assembly. The Al(2)O(3) nanodots act as highly resistant plasma etching, and are used pattern high aspect ratio (>10) silicon nanowires nanopillars.
The tyssue Python library seeks to provide a unified interface implement bio-mechanical models of living tissues.Its main focus is on vertex based epithelium models.tyssue allows model the mechanical behavior 2D, apical 3D or full epithelia numerical resolution equations motion for mesh vertices.Biological processes are modeled through changes in topological and dynamical properties mesh.tyssue modular library.Starting with same tissue geometry, choice constraints, energy potential terms...
The study of vesicles in suspension is important to understand the complicated dynamics exhibited by cells vivo and vitro. We developed a computer simulation based on boundary-integral method model three dimensional gravity-driven sedimentation charged towards flat surface. membrane mechanical behavior was modeled using Helfrich Hamiltonian near incompressibility enforced via which accounts for thermal fluctuations membrane. simulations were verified compared experimental data obtained...
In eukaryotic cells, proper formation of the spindle is necessary for successful cell division. For faithful segregation sister chromatids, each kinetochore must attach to microtubules that extend opposite poles (chromosome bi-orientation). At metaphase-anaphase transition, cohesion between chromatids removed, and chromatid pulled by microtubule-dependent forces.We have studied role minus-end-directed motor protein dynein analysing dynamics in fission yeast cells deleted heavy chain (Dhc1)...
We apply the technique of far-field interferometry to measure properties surface waves generated by two-dimensional (2D) single subwavelength slit-groove structures on gold films. The effective index refraction n(surf) measured for wave propagating over a distance more than 12 mum is determined be = 1.016+/-0.004, within experimental uncertainty close expected bound plasmon-polariton (SPP) value Au/Air interface n (spp) 1.018. compare these measurements finite-difference-time-domain (FDTD)...
Current microfluidic techniques are powerful to study bacteria and determine their response antibiotic treatment, but they currently limited by complex manipulation. Chitosan films fully biocompatible could thus be a viable replacement for existing commercial devices that use polylysine. Thus, the low cost of chitosan slides simple implementation make them highly versatile research as well clinical use.
The directed self-assembly (DSA) of block copolymers (BCPs) is a powerful method for the manufacture high-resolution features. Critical issues remain to be addressed successful implementation DSA, such as dewetting and controlled orientation BCP domains through physicochemical manipulations at interfaces, spatial positioning registration Here, we introduce novel top-coat (TC) materials designed undergo cross-linking reactions triggered by thermal or photoactivation processes. cross-linked TC...
We analyze the physical-chemical surface properties of single-slit, single-groove subwavelength-structured silver films with high-resolution transmission electron microscopy and calculate exact solutions to Maxwell's equations corresponding recent far-field interferometry experiments using these structures. Contrary a suggestion analysis shows that are free detectable contaminants. The finite-difference time-domain calculations, in excellent agreement experiment, show rapid fringe amplitude...
Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Twitter Facebook Reddit LinkedIn Tools Reprints and Permissions Cite Search Site Citation G. Gay, D. Belhachemi, J. P. Colonna, S. Minoret, Brianceau, Lafond, T. Baron, Molas, E. Jalaguier, A. Beaurain, B. Pelissier, V. Vidal, De Salvo; Passivated TiN nanocrystals/SiN trapping layer for enhanced erasing in nonvolatile memory. Appl. Phys. Lett. 11 October 2010; 97 (15): 152112....