A5104581616- Cellular Mechanics and Interactions
- Developmental Biology and Gene Regulation
- Hippo pathway signaling and YAP/TAZ
- Microtubule and mitosis dynamics
- 3D Printing in Biomedical Research
- Cell death mechanisms and regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Microbial Natural Products and Biosynthesis
- Plant biochemistry and biosynthesis
- Phagocytosis and Immune Regulation
- Neurobiology and Insect Physiology Research
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Wnt/β-catenin signaling in development and cancer
- Silk-based biomaterials and applications
- Genetics, Aging, and Longevity in Model Organisms
- Microbial Metabolic Engineering and Bioproduction
- Hemispheric Asymmetry in Neuroscience
- Microfluidic and Bio-sensing Technologies
- Micro and Nano Robotics
- Tissue Engineering and Regenerative Medicine
- Invertebrate Immune Response Mechanisms
- Autophagy in Disease and Therapy
- Animal Genetics and Reproduction
- Planarian Biology and Electrostimulation
Université de Toulouse
2015-2025
Université Toulouse III - Paul Sabatier
2016-2025
Centre National de la Recherche Scientifique
2016-2025
Laboratoire de Microbiologie et Génétique Moléculaires
2001-2024
Laboratoire de Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération
2012-2023
Centre de Biologie du Développement
1997-2022
Joint BioEnergy Institute
2011-2013
Lawrence Berkeley National Laboratory
2011-2013
Universidad Autónoma de Madrid
2001-2013
Inserm
2012-2013
Dissecting Megaenzyme Mechanisms Filamentous fungi contain a class of multidomain enzymes, the highly-reducing iterative polyketide synthases (HR-IPKSs), which produce important natural products such as cholesterol-lowering drug lovastatin. To their complex products, these megasynthases use multiple catalytic domains repeatedly in different combinations, but mechanistic details remain unclear. Ma et al. (p. 589 ) now report vitro reconstitution complete function lovastatin nonaketide...
Epithelial monolayers are two-dimensional cell sheets which compartmentalize the body and organs of multicellular organisms. Their morphogenesis during development or pathology results from patterned endogenous exogenous forces their interplay with tissue mechanical properties. In particular, bending epithelia is thought to result active torques generated by polarization myosin motors along apicobasal axis. However, contribution these out-of-plane remains challenging evaluate because lack...
Current super-resolution microscopy (SRM) methods suffer from an intrinsic complexity that might curtail their routine use in cell biology. We describe here random illumination (RIM) for live-cell imaging at super-resolutions matching of 3D structured microscopy, a robust fashion. Based on speckled and statistical image reconstruction, easy to implement user-friendly, RIM is unaffected by optical aberrations the excitation side, linear brightness, compatible with multicolor over extended...
Abstract In Drosophila, the Jun-N-terminal Kinase-(JNK) signaling pathway is required for epithelial cell shape changes during dorsal closure of embryo. absence JNK activity, as in DJNKK/hemipterous (hep) mutant, dorsolateral ectodermal cells fail both to elongate and move toward midline, leading dorsally open embryos. We show here that hep are later development, correct morphogenesis other epithelia, imaginal discs. During metamorphosis, discs undergo profound morphological changes, giving...
Far from being passive, apoptotic cells influence their environment. For example, they promote tissue folding, myoblast fusion and modulate tumor growth. Understanding the role of necessitates efficient tracking within living tissues, a task that is currently challenging. In order to easily spot in developing Drosophila we generated series fly lines expressing different fluorescent sensors caspase activity. We show three these reporters (GFP-, Cerulean- Venus-derived molecules) are detected...
Epithelial-mesenchymal transition (EMT) is an essential process both in physiological and pathological contexts. Intriguingly, EMT often associated with tissue invagination during development; however, the impact of on remodeling remain unexplored. Here, we show that at initiation process, cells produce apico-basal force, orthogonal to surface epithelium, constitutes important driving force for Drosophila. When ectopically induced, starting their delamination generate induce ectopic folding....
Mechanical forces are critical regulators of cell shape changes and developmental morphogenetic processes. Forces generated along the epithelium apico-basal axis have recently emerged as essential for tissue remodeling in three dimensions. Yet cellular machinery underlying those orthogonal remains poorly described. We found that during Drosophila leg folding cells eventually committed to die produce through formation a dynamic actomyosin contractile tether connecting apical surface basally...
In bilateria, positioning and looping of visceral organs requires proper left-right (L/R) asymmetry establishment. Recent work in Drosophila has identified a novel situs inversus gene encoding the unconventional type ID myosin (MyoID). myoID mutant flies, L/R axis is inverted, causing reversed organs, such as gut, spermiduct genitalia. We have previously shown that MyoID interacts physically with β-Catenin, suggesting role adherens junction asymmetry. Here, we show DE-Cadherin...
Apoptosis plays an important role in morphogenesis, and the notion that apoptotic cells can impact their surroundings came to light recently. However, how this applies vertebrate morphogenesis remains unknown. Here, we use formation of neural tube determine apoptosis contributes vertebrates. Neural closure defects have been reported when is impaired vertebrates, although cellular mechanisms involved are Using avian embryos, found generate apico-basal force before being extruded from...
Iterative fungal polyketide synthases (PKSs) use a unique set of biochemical rules in the synthesis complex polyketides. These dictate starter unit selection, chain length control, and post-PKS processing. We have demonstrated E. coli expression reconstitution an iterative, unreduced PKS. The Gibberella fujikuroi PKS4 was expressed at high levels, purified to homogeneity, functionally characterized. In presence malonyl-CoA, able synthesize nonaketide...
Abstract Background Microbial engineering strategies that elicit global metabolic perturbations have the capacity to increase organism robustness for targeted metabolite production. In particular, regulators of cellular systems impact glycolysis and amino acid production while simultaneously decreasing fermentation by-products such as acetate CO 2 make ideal targets. Intriguingly, perturbation Carbon Storage Regulator (Csr) system has been previously implicated in large changes central...
Major efforts in bioenergy research have focused on producing fuels that can directly replace petroleum-derived gasoline and diesel fuel through metabolic engineering of microbial fatty acid biosynthetic pathways. Typically, growth pathway induction are conducted under aerobic conditions, but for operational efficiency an industrial context, anaerobic culture conditions would be preferred to obviate the need maintain specific dissolved oxygen concentrations maximize proportion reducing...
Tissue mechanics play a crucial role in organ development. They rely on the properties of cells and extracellular matrix (ECM), but relative physical contribution ECM to morphogenesis is poorly understood. Here, we have analyzed behavior peripodial epithelium (PE) Drosophila leg disc light dynamics its cellular components. The PE undergoes successive changes during development, including elongation, opening removal free leg. During found that cell layer are progressively uncoupled....