A5077988767- Platelet Disorders and Treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Cellular transport and secretion
- Blood Coagulation and Thrombosis Mechanisms
- Calcium signaling and nucleotide metabolism
- Cell Adhesion Molecules Research
- Blood properties and coagulation
- Ubiquitin and proteasome pathways
- PI3K/AKT/mTOR signaling in cancer
- Extracellular vesicles in disease
- Pancreatic function and diabetes
- Chronic Lymphocytic Leukemia Research
- Erythrocyte Function and Pathophysiology
- Venous Thromboembolism Diagnosis and Management
- Drug-Induced Adverse Reactions
- Immune Cell Function and Interaction
- Cholesterol and Lipid Metabolism
- Hereditary Neurological Disorders
- Angiogenesis and VEGF in Cancer
- Heparin-Induced Thrombocytopenia and Thrombosis
- interferon and immune responses
- Sphingolipid Metabolism and Signaling
- Metabolism, Diabetes, and Cancer
- Protease and Inhibitor Mechanisms
- Lipid metabolism and biosynthesis
Inserm
2010-2023
Institut des Maladies Métaboliques et Cardiovasculaires
2014-2023
Université Toulouse III - Paul Sabatier
2014-2023
Laboratoire d'Aérologie
2023
Université de Toulouse
2011
Hôpital Purpan
1997-2000
Phosphoinositides represent a major class of lipids specifically involved in the organisation signaling cascades, maintenance identity organelles and regulation multiple intracellular trafficking steps. We previously described that phosphatidylinositol 5-monophosphate (PI5P), produced by Shigella flexneri phosphatase IpgD, is implicated endosomal sorting EGFR. Here, we show adaptor protein TOM1 new PI5P direct binding partner. identify domain this interaction characterize motif. Finally,...
Circulating microparticles derived from different types of blood cells have been reported to impair endothelial function and induce pro-inflammatory prothrombotic phenotypes. Although the number monocyte-derived (M-MPs) is elevated in patients with various inflammatory conditions, their interaction has poorly investigated so far. In this study, we produced vitro apoptotic human monocytes examined effects cultured umbilical vascular (HUVECs). We found that low concentrations M-MPs induced...
Myotubularins (MTMs) are active or dead phosphoinositides phosphatases defining a large protein family conserved through evolution and implicated in different neuromuscular diseases. Loss-of-function mutations MTM1 cause the severe congenital myopathy called myotubular (or X-linked centronuclear myopathy) while MTM1-related MTMR2 recessive Charcot-Marie-Tooth peripheral neuropathy. Here we aimed to determine functional specificity redundancy of MTMR2, assess their abilities compensate for...
Summary Tissue Factor (TF), the receptor for plasma VII/VIIa and initiator of blood coagulation, is inducible in vascular smooth muscle cells (SMCs) by growth factors bacterial lysopolysaccharides (LPS) expressed vivo after injury. As TF expression a determinant thrombogenicity lesions, we investigated signal pathways involved this process. Human SMCs were obtained from normal arteries made quiescent serum deprivation. Baseline antigen activity up-regulated various agonists: fetal calf...
Secretory granules are key elements for platelet functions. Their biogenesis and integrity regulated by fine-tuned mechanisms that need to be fully characterized. Here, we investigated the role of phosphoinositide 5-kinase PIKfyve its lipid products, PtdIns5P (phosphatidylinositol 5 monophosphate) PtdIns(3,5)P2 (3,5) bisphosphate) in granule homeostasis megakaryocytes platelets.For that, invalidated pharmacological inhibition or gene silencing megakaryocytic cell models (human MEG-01 line,...
Summary Human vascular smooth muscle cells (SMCs) isolated from normal adult arteries were investigated for the expression of Tissue Factor (TF) procoagulant activity at their membrane surface and TF antigen in lysed cells. In growing conditions, SMCs expressed high levels antigen. When made quiescent by 72 h subculture serum-poor medium, these fell to about one third initial values. Platelet-derived growth factor BB (PDGF) up-regulated a dose-dependent manner with significant increase (1.8...
Platelet protease-activated receptor 1 (PAR1) is a cell surface G-protein-coupled (GPCR) that acts as thrombin promoting platelet aggregation. Targeting the PAR1 pathway by vorapaxar, antagonist, leads to reduction in ischemic events cardiovascular patients with history of myocardial infarction or peripheral arterial disease. In platelets, specialized microdomains highly enriched cholesterol act modulators activity several GPCRs and play pivotal role signaling pathway. However, their...
Summary Smooth muscle cells (SMCs) of the intima are generally quiescent and non proliferative. Their proliferation due to different stimulations occurs in myointimal hyperplasia is regularly present athero-genesis or after transluminal angioplasty leading vascular occlusive stenosis. In course these pathologies, Tissue Factor (TF) synthesis was upregulated rapidly expressed at membrane SMCs. Heparin known inhibit SMCs induced by FCS. We evaluated inhibitory effect heparin on expression TF...
Blood platelets are produced by megakaryocytes through a complex program of differentiation and play critical role in hemostasis thrombosis. These anucleate cells the target antithrombotic drugs that prevent them from clumping cardiovascular diseases conditions. Platelets also significantly contribute to various aspects physiopathology, including interorgan communications, healing, inflammation, thromboinflammation. Their production activation strictly regulated highly elaborated mechanisms....