A5073845826- Platelet Disorders and Treatments
- PI3K/AKT/mTOR signaling in cancer
- Cellular transport and secretion
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Antiplatelet Therapy and Cardiovascular Diseases
- Mast cells and histamine
- Chronic Lymphocytic Leukemia Research
- Blood properties and coagulation
- Cell Adhesion Molecules Research
- Renal Diseases and Glomerulopathies
- Pancreatic function and diabetes
- Chronic Myeloid Leukemia Treatments
- Venous Thromboembolism Diagnosis and Management
- Lipid metabolism and disorders
- Complement system in diseases
- Autophagy in Disease and Therapy
- Erythrocyte Function and Pathophysiology
- Autoimmune Bullous Skin Diseases
- Veterinary Oncology Research
- Sphingolipid Metabolism and Signaling
- Menopause: Health Impacts and Treatments
- Cytokine Signaling Pathways and Interactions
- Gender, Education, and Development Issues
- Kruppel-like factors research
- Human Health and Disease
Institut des Maladies Métaboliques et Cardiovasculaires
2015-2024
Inserm
2014-2024
Université Toulouse III - Paul Sabatier
2014-2024
Université de Toulouse
2012-2019
University College London
2015
University of Birmingham
2009-2013
NIHR Birmingham Biomedical Research Centre
2012
Centre de Physiopathologie de Toulouse-Purpan
2007
Community College of Philadelphia
2005
Colorado State University
1999
The C-type lectin-like receptor CLEC-2 signals via phosphorylation of a single cytoplasmic YXXL sequence known as hem-immunoreceptor tyrosine-based activation motif (hemITAM). In this study, we show that by the snake toxin rhodocytin is abolished in absence tyrosine kinase Syk but not altered major platelet Src family kinases, Fyn, Lyn, and Src, or phosphatase CD148, which regulates basal activity kinases. Further, presence inhibitor PP2, even though PLCγ2 are abolished. A similar dependence...
Megakaryopoiesis and platelet formation is a multistep process through which hematopoietic progenitor cells develop into mature megakaryocytes (MKs) form proplatelets. The present study investigates the regulation of different steps megakaryopoiesis (i.e., differentiation, migration, proplatelet formation) by extracellar signal-regulated kinase (ERK)1/2 p38 mitogen-activated protein (MAPK) in two models primary murine MKs derived from bone marrow (BM) fetal liver (FL) cells.A preparation was...
Megakaryocytes (MKs) come from a complex process of hematopoietic progenitor maturation within the bone marrow that gives rise to de novo circulating platelets. Bone microenvironment contains large number adipocytes with still ill-defined role. This study aims analyze influence and increased medullar adiposity in megakaryopoiesis. An vivo mice caused by high-fat-diet-induced obesity is associated an enhanced MK proplatelet formation. In vitro co-culture progenitors shows delipidation...
Platelets are critical for normal hemostasis. Their deregulation can lead to bleeding or arterial thrombosis, a primary cause of heart attack and ischemic stroke. Src homology 2 domain-containing inositol 5-phosphatase 1 (SHIP1) is capable dephosphorylating the phosphatidylinositol 3,4,5-trisphosphate second messenger into 3,4-bisphosphate. SHIP1 plays role in regulating level these lipids platelets. Using SHIP1-deficient mice, we found that its loss affects platelet aggregation response...
Abstract Phosphatidylinositol 3-kinase type 2α (PI3KC2α) and related class II PI3K isoforms are of increasing biomedical interest because their crucial roles in endocytic membrane dynamics, cell division signaling, angiogenesis, platelet morphology function. Herein we report the development characterization PhosphatidylInositol Three-kinase Class twO INhibitors (PITCOINs), potent highly selective small-molecule inhibitors PI3KC2α catalytic activity. PITCOIN compounds exhibit strong...
PtdIns3P is recognized as an important player in the control of endocytotic pathway and autophagy. Recent data also suggest that contributes to molecular mechanisms taking place at plasma membrane midbody during cytokinesis. This lipid present low amounts mammalian cells remains difficult quantify either by traditional techniques based on radiolabelling followed HPLC separate different phosphatidylinositol monophosphates, or high-sensitive liquid chromatography coupled MS, which still under...
Objective— PI3Kα (phosphoinositide 3-kinase alpha) is a therapeutic target in oncology, but its role platelets and thrombosis remains ill characterized. In this study, we have analyzed the of vitro, ex vivo, vivo 2 models arterial thrombosis. Approach Results— Using mice selectively deficient p110α megakaryocyte lineage isoform-selective inhibitors, confirm that not mandatory participates to thrombus growth over collagen matrix at shear rate. Our data uncover for low-level activation GP...
Arterial thrombosis causes heart attacks and most strokes is the common cause of death in world. Platelets are cells that form arterial thrombi, antiplatelet drugs mainstay attack stroke prevention. Yet, current have limited efficacy, preventing fewer than 25% lethal cardiovascular events without clinically relevant effects on bleeding. The key limitation ability all to impair causing bleeding they block global platelet activation, thereby indiscriminately function hemostasis thrombosis....
BackgroundPlatelet endothelial aggregation receptor 1 (PEAR1) is a single-transmembrane orphan primarily expressed on platelets and cells. Genetic variants of PEAR1 have repeatedly independently been identified to be associated with cardiovascular diseases, including coronary artery disease.ObjectivesWe sulfated fucoidans their mimetics as ligands for proposed that its endogenous ligand proteoglycan. The aim this study was test hypothesis.MethodsA heparin proteoglycan-mimetic (HPGM) created...