A5029919195- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Cellular transport and secretion
- Ubiquitin and proteasome pathways
- Advanced Biosensing Techniques and Applications
- Amyotrophic Lateral Sclerosis Research
- Proteoglycans and glycosaminoglycans research
- Cell Adhesion Molecules Research
- Neurogenetic and Muscular Disorders Research
- Signaling Pathways in Disease
- Peptidase Inhibition and Analysis
- Viral Infectious Diseases and Gene Expression in Insects
- Alzheimer's disease research and treatments
- Digital Imaging for Blood Diseases
- RNA Research and Splicing
- Endoplasmic Reticulum Stress and Disease
- Pancreatic function and diabetes
- Cancer-related gene regulation
- Cellular Mechanics and Interactions
- Machine Learning in Bioinformatics
- Cancer Genomics and Diagnostics
- Genetic Neurodegenerative Diseases
- Adenosine and Purinergic Signaling
- Chronic Lymphocytic Leukemia Research
- Protease and Inhibitor Mechanisms
Montreal Neurological Institute and Hospital
2020-2025
McGill University
2018-2025
Structural Genomics Consortium
2021-2024
Developmental Studies Hybridoma Bank
2024
University of Toronto
2024
Cell Signaling Technology (United States)
2024
University of Leicester
2024
Emory University
2024
Institute for Research in Immunology and Cancer
2012-2016
Université de Montréal
2012-2016
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of problem remains largely anecdotal, as such, feasibility goal at least one potent specific antibody targeting each protein in a proteome cannot be assessed. Focusing for human proteins, we have scaled standardized characterization approach using parental knockout cell lines (Laflamme et al.,...
Antibodies are a key resource in biomedical research yet there no community-accepted standards to rigorously characterize their quality. Here we develop procedure validate pre-existing antibodies. Human cell lines with high expression of target, determined through proteomics database, modified CRISPR/Cas9 knockout (KO) the corresponding gene. Commercial antibodies against target purchased and tested by immunoblot comparing parental KO. Validated used definitively identify most highly...
TBK1 is a serine-threonine protein kinase that has been linked to number of diseases including amyotrophic lateral sclerosis and frontotemporal dementia. Reproducible research on hampered by the lack well characterized antibodies. In this study, we 11 commercial antibodies for use in immunoblot, immunofluorescence immunoprecipitation, using an isogeneic knock-out cell line as control. We identify appear specific all three applications but invite readers interpret present findings based their...
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of problem remains largely anecdotal, as such, feasibility goal at least one potent specific antibody targeting each protein in a proteome cannot be assessed. Focusing for human proteins, we have scaled standardized characterization approach using parental knockout cell lines (Laflamme et al.,...
Abstract Antibody-based research applications are critical for biological discovery. Yet, there no industry standards to compare the performance of antibodies in various applications. We describe a knockout cell line-based antibody characterization platform, developed, and approved jointly by academic researchers that enables systematic comparison western blot, immunoprecipitation, immunofluorescence procedures. The scalable protocols consist (i) identification appropriate lines studies,...
<ns3:p>Moesin is a cytoskeletal adaptor protein, involved in the modification of actin cytoskeleton, with relevance to Alzheimer’s Disease. Well characterized anti-Moesin antibodies would benefit scientific community. In this study, we have ten Moesin commercial Western Blot, immunoprecipitation, and immunofluorescence using standardized experimental protocol based on comparing read-outs knockout cell lines isogenic parental controls. These studies are part larger, collaborative initiative...
<ns3:p>Sequestosome-1, encoded by the gene <ns3:italic>SQSTM1</ns3:italic>, functions as a bridge between ubiquitinated proteins and proteasome or autophagosome, thereby regulating protein degradation pathways. Loss of Sequestosome-1 is hypothesized to enhance neurodegeneration progression in several diseases, including amyotrophic lateral sclerosis (ALS) frontotemporal disorders (FTD). reproducible research would be facilitated with availability well-characterized anti-Sequestosome-1...
Abstract Background The CD133(+) stem cell population in recurrent gliomas is associated with clinical features such as therapy resistance, blood-brain barrier disruption and, hence, tumor infiltration. Screening of a large panel glioma samples increasing histological grade demonstrated frequencies cells which correlated high expression cyclooxygenase (COX)-2 and membrane type-1 matrix metalloproteinase (MT1-MMP). Methods We used qRT-PCR immunoblotting to examine the molecular interplay...
Membrane trafficking has well-defined roles during cell migration. However, its regulation is poorly characterized. In this paper, we describe the first screen for putative Rab–GTPase-activating proteins (GAPs) collective migration of Drosophila melanogaster border cells (BCs), identify uncharacterized protein Evi5 as an essential membrane regulator, and molecular mechanism by which regulates BC requires Rab-GAP activity to fulfill functions acts a GAP Rab11. Both loss gain function blocked...
<ns4:p>The enzyme stearoyl-CoA desaturase (SCD1) is a modulator of lipid metabolism by catalyzing the biosynthesis mono-unsaturated fatty acids from saturated acids. Understanding specific mechanisms which SCD1 plays in health and disease can provide novel insides therapeutic targets, process that would be facilitated availability high-quality antibodies. Here we have characterized nine commercial antibodies for western blot, immunoprecipitation, immunofluorescence using standardized...
<ns4:p>Huntingtin encodes a 3144 amino acid protein, with polyglutamine repeat tract at the N-terminus. Expansion of this above pathogenic threshold 36 repeats is causative mutation Huntington's disease, neurodegenerative disorder characterized by loss striatal neurons. Here we have twenty Huntingtin commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using standardized experimental protocol based on comparing read-outs in knockout cell lines isogenic parental...
<ns4:p>Stimulator of interferon genes protein (STING1) is an immune adaptor which promotes innate defense mechanisms against pathogens. To enhance our understanding STING1-associated disease, it essential to make high-performing antibodies accessible the scientific community. This study aims improve reliability STING1 research as we have characterized sixteen commercial for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing...
<ns3:p>TAF15 (TATA-box binding protein-associated factor 15) is a member of the FET protein family, known for their roles in transcriptional regulation and RNA metabolism. Here we have characterized five TAF15 commercial antibodies western blot, immunoprecipitation, immunofluorescence using standardized experimental protocol based on comparing read-outs knockout cell lines isogenic parental controls. These studies are part larger, collaborative initiative seeking to address antibody...
<ns4:p>Sphingosine 1-phosphate receptor 1 (S1PR1) is a G-coupled protein that induces crucial biological processes when bound by sphingosine 1-phosphate. Here, we have characterized nine S1PR1 commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using standardized experimental protocol based on comparing read-outs in knockout cell lines isogenic parental controls. These studies are part of larger, collaborative initiative seeking to address antibody...
<ns3:p>Rab1 is a highly conserved small GTPase that exists in humans as two isoforms: Rab1A and Rab1B, sharing 92% sequence identity. These proteins regulate vesicle trafficking between the endoplasmic reticulum (ER) Golgi within stacks. Rab1B may be oncogenes, they are frequently dysregulated various human cancers. Moreover, contribute to progression of Parkinson’s disease. The availability high-quality antibodies specific for or essential understand distinct functions these Rab1 both...
Intronic hexanucleotide repeat expansions in the C9orf72 gene represent most common genetic cause of neurodegenerative diseases amyotrophic lateral sclerosis (ALS) and frontotemporal dementia. This expansion decreases expression affected patients, indicating that loss function (LOF) acts as a pathogenic mechanism. Several models using Danio rerio (zebrafish) for depletion have been developed to explore disease mechanisms consequences LOF. However, inconsistencies exist reported phenotypes,...
Abstract The neural precursor surface marker CD133 is thought to be enriched in brain cancer stem cells and radioresistant DAOY medulloblastoma-derived tumor cells. Given that membrane type-1 matrix metalloproteinase (MT1-MMP) expression a hallmark of highly invasive, radioresistant, hypoxic cells, we sought determine whether MT1-MMP other MMPs could regulate the invasive phenotype CD133(+) We found when medulloblastoma or U87 glioblastoma were implanted nude mice, only those specifically...
<ns3:p>Sequestosome-1, encoded by the gene <ns3:italic>SQSTM1</ns3:italic>, functions as a bridge between ubiquitinated proteins and proteasome or autophagosome, thereby regulating protein degradation pathways. Loss of Sequestosome-1 is hypothesized to enhance neurodegeneration progression in several diseases, including amyotrophic lateral sclerosis (ALS) frontotemporal disorders (FTD). reproducible research would be facilitated with availability well-characterized anti-Sequestosome-1...
<ns4:p>Ubiquilin-2, a member of the ubiquilin protein family, plays role in regulation various degradation pathways, and is mutated some neurodegenerative diseases. Well-characterized anti-Ubiquilin-2 antibodies would advance reproducible research for Ubiquilin-2 turn, benefit scientific community. In this study, we characterized ten commercial Western Blot, immunoprecipitation, immunofluorescence using standardized experimental protocol based on comparing read-outs knockout cell lines...
Transmembrane protein 106B (TMEM106B), a that is localized to the lysosome, genetically linked many neurodegenerative diseases and forms fibrils in diseased brains. The reproducibility of TMEM106B research would be enhanced if community had access well-characterized anti-TMEM106B antibodies. In this study, we characterized six commercially available antibodies for their performance Western blot, immunoprecipitation, immunofluorescence, using standardized experimental protocol based on...