A5000907877- Monoclonal and Polyclonal Antibodies Research
- Cellular transport and secretion
- Advanced Biosensing Techniques and Applications
- Endoplasmic Reticulum Stress and Disease
- Erythrocyte Function and Pathophysiology
- RNA and protein synthesis mechanisms
- Pancreatic function and diabetes
- Sphingolipid Metabolism and Signaling
- Genetic Neurodegenerative Diseases
- Neuroscience and Neural Engineering
- Bone and Dental Protein Studies
- Protein purification and stability
- Lysosomal Storage Disorders Research
- Photoreceptor and optogenetics research
- Mitochondrial Function and Pathology
- Ubiquitin and proteasome pathways
- Polymer Surface Interaction Studies
- Blood properties and coagulation
- Oral and gingival health research
- Gold and Silver Nanoparticles Synthesis and Applications
- Lymphoma Diagnosis and Treatment
- Advanced Proteomics Techniques and Applications
- vaccines and immunoinformatics approaches
- Bone Metabolism and Diseases
- Alzheimer's disease research and treatments
Montreal Neurological Institute and Hospital
2023-2025
McGill University
2023-2025
Developmental Studies Hybridoma Bank
2024
University of Toronto
2024
University of Leicester
2024
Cell Signaling Technology (United States)
2024
Emory University
2024
Structural Genomics Consortium
2023-2024
Institute of Photonic Sciences
2021-2023
Université de Montréal
2022
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of problem remains largely anecdotal, as such, feasibility goal at least one potent specific antibody targeting each protein in a proteome cannot be assessed. Focusing for human proteins, we have scaled standardized characterization approach using parental knockout cell lines (Laflamme et al.,...
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of problem remains largely anecdotal, as such, feasibility goal at least one potent specific antibody targeting each protein in a proteome cannot be assessed. Focusing for human proteins, we have scaled standardized characterization approach using parental knockout cell lines (Laflamme et al.,...
Abstract Antibody-based research applications are critical for biological discovery. Yet, there no industry standards to compare the performance of antibodies in various applications. We describe a knockout cell line-based antibody characterization platform, developed, and approved jointly by academic researchers that enables systematic comparison western blot, immunoprecipitation, immunofluorescence procedures. The scalable protocols consist (i) identification appropriate lines studies,...
<ns4:p>The enzyme stearoyl-CoA desaturase (SCD1) is a modulator of lipid metabolism by catalyzing the biosynthesis mono-unsaturated fatty acids from saturated acids. Understanding specific mechanisms which SCD1 plays in health and disease can provide novel insides therapeutic targets, process that would be facilitated availability high-quality antibodies. Here we have characterized nine commercial antibodies for western blot, immunoprecipitation, immunofluorescence using standardized...
<ns4:p>Huntingtin encodes a 3144 amino acid protein, with polyglutamine repeat tract at the N-terminus. Expansion of this above pathogenic threshold 36 repeats is causative mutation Huntington's disease, neurodegenerative disorder characterized by loss striatal neurons. Here we have twenty Huntingtin commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using standardized experimental protocol based on comparing read-outs in knockout cell lines isogenic parental...
<ns4:p>Sphingosine 1-phosphate receptor 1 (S1PR1) is a G-coupled protein that induces crucial biological processes when bound by sphingosine 1-phosphate. Here, we have characterized nine S1PR1 commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using standardized experimental protocol based on comparing read-outs in knockout cell lines isogenic parental controls. These studies are part of larger, collaborative initiative seeking to address antibody...
<ns3:p>Rab1 is a highly conserved small GTPase that exists in humans as two isoforms: Rab1A and Rab1B, sharing 92% sequence identity. These proteins regulate vesicle trafficking between the endoplasmic reticulum (ER) Golgi within stacks. Rab1B may be oncogenes, they are frequently dysregulated various human cancers. Moreover, contribute to progression of Parkinson’s disease. The availability high-quality antibodies specific for or essential understand distinct functions these Rab1 both...
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of problem remains largely anecdotal, as such, feasibility goal at least one potent specific antibody targeting each protein in a proteome cannot be assessed. Focusing for human proteins, we have scaled standardized characterization approach using parental knockout cell lines (Laflamme et al.,...
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of problem remains largely anecdotal, as such, feasibility goal at least one potent specific antibody targeting each protein in a proteome cannot be assessed. Focusing for human proteins, we have scaled standardized characterization approach using parental knockout cell lines (Laflamme et al.,...
CSNK1A1 is a key regulator of various signalling pathways, including the Wnt/β-catenin pathway. Playing central role in cellular function and disease pathology, has emerged as an attractive protein target for therapeutic development. In this study we characterize ten commercial antibodies western blot, immunoprecipitation, immunofluorescence using standardized experimental protocol based on comparing read-outs knockout cell lines isogenic parental controls. This part larger, collaborative...
Macromolecular bottlebrushes (MBs) are emerging as an attractive polymer architecture for biomedical applications. Herein, synthesis and characterization of sulfoxide-containing water-soluble MBs with poly(2-(methylsulfinyl)ethyl acrylate) (PMSEA) side chains varying grafting densities reported. Highly hydrophilic PMSEA were prepared by grafting-from poly(2-bromoisobutyryloxyethyl methacrylate) (PBiBEM) using photoATRP either CuBr2/TPMA (tris(2-pyridylmethyl)amine), CuBr2/TPMA*3...
Deficiencies in neurotransmission lead to neurological disorders or misinterpretation of perceived threats. To restore defects cellular communication, we developed a synthetic, photon-assisted synaptic transmission (PhAST) system. PhAST is based on luciferases and channelrhodopsins that enable the neuronal state across space, using photons as neurotransmitters. We demonstrate ability overcome barriers rescue behavioral deficit genetically engineered glutamate mutant with conditional, Ca 2+...
Protein-glutamine gamma-glutamyltransferase 2 (TGM2) is a Ca
<ns3:p>SPARC-related modular calcium-binding protein 1, otherwise known as SMOC-1, is a secreted glycoprotein involved in various cell biological processes including cell-matrix interactions, osteoblast differentiation, embryonic development, and homeostasis. SMOC-1 was found to be elevated asymptomatic Alzheimer’s disease (AD) patient cortex well being enriched amyloid plaques AD patientcerebrospinal fluid, arguing for promising biomarker AD. Having access high-quality antibodies crucial...
<ns3:p>VAPB is an adaptor protein known for its role as anchor other proteins at the endoplasmic reticulum. A mutant form of VAPB has been linked to amyotrophic lateral sclerosis and underlying mechanisms resulting from this defect are studied by researchers in area uncover implication disease. Here we have characterized six commercial antibodies western blot, immunoprecipitation, immunofluorescence using a standardized experimental protocol based on comparing read-outs knockout cell lines...
<ns3:p>Protein-glutamine gamma-glutamyltransferase 2 (TGM2) is a Ca<ns3:sup>2+</ns3:sup> dependent enzyme that catalyzes transglutaminase cross-linking modifications. TGM2 involved in various diseases, either protective or contributory manner, making it crucial protein to study and determine its therapeutic potential. Identifying high-performing antibodies would facilitate these investigations. Here we have characterized seventeen commercial for western blot sixteen immunoprecipitation,...
<ns3:p>Sphingosine 1-phosphate receptor 1 (S1PR1) is a G-coupled protein that induces crucial biological processes when bound by sphingosine 1-phosphate. Here, we have characterized nine S1PR1 commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using standardized experimental protocol based on comparing read-outs in knockout cell lines isogenic parental controls. These studies are part of larger, collaborative initiative seeking to address antibody...
<ns3:p>Huntingtin encodes a 3144 amino acid protein, with polyglutamine repeat tract at the N-terminus. Expansion of this above pathogenic threshold 36 repeats is causative mutation Huntington's disease, neurodegenerative disorder characterized by loss striatal neurons. Here we have twenty Huntingtin commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using standardized experimental protocol based on comparing read-outs in knockout cell lines isogenic parental...
<ns4:p>Sphingosine 1-phosphate receptor 1 (S1PR1) is a G-coupled protein that induces crucial biological processes when bound by sphingosine 1-phosphate. Here, we have characterized nine S1PR1 commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using standardized experimental protocol based on comparing read-outs in knockout cell lines isogenic parental controls. These studies are part of larger, collaborative initiative seeking to address antibody...
<ns3:p>ADNP is a multifunctional protein involved in chromatin remodeling, transcription, and microtubule interaction, playing critical role brain development, with mutations linked to <ns3:italic>ADNP</ns3:italic>-Related Disorder. Here we have characterized seven ADNP commercial antibodies for western blot, immunoprecipitation, immunofluorescence using standardized experimental protocol based on comparing read-outs knockout cell lines isogenic parental controls. These studies are part of...