A5078967369- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Cellular transport and secretion
- Ubiquitin and proteasome pathways
- Amyotrophic Lateral Sclerosis Research
- Signaling Pathways in Disease
- Proteoglycans and glycosaminoglycans research
- Alzheimer's disease research and treatments
- Cell Adhesion Molecules Research
- Advanced Biosensing Techniques and Applications
- Neurogenetic and Muscular Disorders Research
- Peptidase Inhibition and Analysis
- Endoplasmic Reticulum Stress and Disease
- Pancreatic function and diabetes
- RNA Research and Splicing
- Cancer-related gene regulation
- Protease and Inhibitor Mechanisms
- Chronic Lymphocytic Leukemia Research
- Viral Infectious Diseases and Gene Expression in Insects
- Cellular Mechanics and Interactions
- Radiopharmaceutical Chemistry and Applications
- Erythrocyte Function and Pathophysiology
- RNA and protein synthesis mechanisms
- Lysosomal Storage Disorders Research
- Amino Acid Enzymes and Metabolism
Montreal Neurological Institute and Hospital
2021-2025
McGill University
2022-2025
Structural Genomics Consortium
2022-2024
Emory University
2024
Developmental Studies Hybridoma Bank
2024
University of Toronto
2024
University of Leicester
2024
Cell Signaling Technology (United States)
2024
Université de Montréal
2017-2021
TBK1 is a serine-threonine protein kinase that has been linked to number of diseases including amyotrophic lateral sclerosis and frontotemporal dementia. Reproducible research on hampered by the lack well characterized antibodies. In this study, we 11 commercial antibodies for use in immunoblot, immunofluorescence immunoprecipitation, using an isogeneic knock-out cell line as control. We identify appear specific all three applications but invite readers interpret present findings based their...
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of problem remains largely anecdotal, as such, feasibility goal at least one potent specific antibody targeting each protein in a proteome cannot be assessed. Focusing for human proteins, we have scaled standardized characterization approach using parental knockout cell lines (Laflamme et al.,...
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of problem remains largely anecdotal, as such, feasibility goal at least one potent specific antibody targeting each protein in a proteome cannot be assessed. Focusing for human proteins, we have scaled standardized characterization approach using parental knockout cell lines (Laflamme et al.,...
Abstract Antibody-based research applications are critical for biological discovery. Yet, there no industry standards to compare the performance of antibodies in various applications. We describe a knockout cell line-based antibody characterization platform, developed, and approved jointly by academic researchers that enables systematic comparison western blot, immunoprecipitation, immunofluorescence procedures. The scalable protocols consist (i) identification appropriate lines studies,...
<ns3:p>Moesin is a cytoskeletal adaptor protein, involved in the modification of actin cytoskeleton, with relevance to Alzheimer’s Disease. Well characterized anti-Moesin antibodies would benefit scientific community. In this study, we have ten Moesin commercial Western Blot, immunoprecipitation, and immunofluorescence using standardized experimental protocol based on comparing read-outs knockout cell lines isogenic parental controls. These studies are part larger, collaborative initiative...
<ns3:p>Sequestosome-1, encoded by the gene <ns3:italic>SQSTM1</ns3:italic>, functions as a bridge between ubiquitinated proteins and proteasome or autophagosome, thereby regulating protein degradation pathways. Loss of Sequestosome-1 is hypothesized to enhance neurodegeneration progression in several diseases, including amyotrophic lateral sclerosis (ALS) frontotemporal disorders (FTD). reproducible research would be facilitated with availability well-characterized anti-Sequestosome-1...
<ns3:p>Sequestosome-1, encoded by the gene <ns3:italic>SQSTM1</ns3:italic>, functions as a bridge between ubiquitinated proteins and proteasome or autophagosome, thereby regulating protein degradation pathways. Loss of Sequestosome-1 is hypothesized to enhance neurodegeneration progression in several diseases, including amyotrophic lateral sclerosis (ALS) frontotemporal disorders (FTD). reproducible research would be facilitated with availability well-characterized anti-Sequestosome-1...
<ns4:p>Ubiquilin-2, a member of the ubiquilin protein family, plays role in regulation various degradation pathways, and is mutated some neurodegenerative diseases. Well-characterized anti-Ubiquilin-2 antibodies would advance reproducible research for Ubiquilin-2 turn, benefit scientific community. In this study, we characterized ten commercial Western Blot, immunoprecipitation, immunofluorescence using standardized experimental protocol based on comparing read-outs knockout cell lines...
Transmembrane protein 106B (TMEM106B), a that is localized to the lysosome, genetically linked many neurodegenerative diseases and forms fibrils in diseased brains. The reproducibility of TMEM106B research would be enhanced if community had access well-characterized anti-TMEM106B antibodies. In this study, we characterized six commercially available antibodies for their performance Western blot, immunoprecipitation, immunofluorescence, using standardized experimental protocol based on...
<ns4:p>The enzyme stearoyl-CoA desaturase (SCD1) is a modulator of lipid metabolism by catalyzing the biosynthesis mono-unsaturated fatty acids from saturated acids. Understanding specific mechanisms which SCD1 plays in health and disease can provide novel insides therapeutic targets, process that would be facilitated availability high-quality antibodies. Here we have characterized nine commercial antibodies for western blot, immunoprecipitation, immunofluorescence using standardized...
<ns4:p>Huntingtin encodes a 3144 amino acid protein, with polyglutamine repeat tract at the N-terminus. Expansion of this above pathogenic threshold 36 repeats is causative mutation Huntington's disease, neurodegenerative disorder characterized by loss striatal neurons. Here we have twenty Huntingtin commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using standardized experimental protocol based on comparing read-outs in knockout cell lines isogenic parental...
<ns4:p>Stimulator of interferon genes protein (STING1) is an immune adaptor which promotes innate defense mechanisms against pathogens. To enhance our understanding STING1-associated disease, it essential to make high-performing antibodies accessible the scientific community. This study aims improve reliability STING1 research as we have characterized sixteen commercial for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing...
<ns3:p>TAF15 (TATA-box binding protein-associated factor 15) is a member of the FET protein family, known for their roles in transcriptional regulation and RNA metabolism. Here we have characterized five TAF15 commercial antibodies western blot, immunoprecipitation, immunofluorescence using standardized experimental protocol based on comparing read-outs knockout cell lines isogenic parental controls. These studies are part larger, collaborative initiative seeking to address antibody...
<ns4:p>Sphingosine 1-phosphate receptor 1 (S1PR1) is a G-coupled protein that induces crucial biological processes when bound by sphingosine 1-phosphate. Here, we have characterized nine S1PR1 commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using standardized experimental protocol based on comparing read-outs in knockout cell lines isogenic parental controls. These studies are part of larger, collaborative initiative seeking to address antibody...
<ns3:p>Rab1 is a highly conserved small GTPase that exists in humans as two isoforms: Rab1A and Rab1B, sharing 92% sequence identity. These proteins regulate vesicle trafficking between the endoplasmic reticulum (ER) Golgi within stacks. Rab1B may be oncogenes, they are frequently dysregulated various human cancers. Moreover, contribute to progression of Parkinson’s disease. The availability high-quality antibodies specific for or essential understand distinct functions these Rab1 both...
Profilin-1, a member of the Profilin family, is ubiquitously expressed protein that controls actin polymerization in concentration-dependent manner. As mutations Profilin-1 gene have potential implications neurodegenerative disease progression, well-characterized anti-Profilin-1 antibodies would be beneficial to scientific community. In this study, we characterized sixteen commercial for Western blot, immunoprecipitation, and immunofluorescence applications, using standardized experimental...
<ns4:p>TAR DNA-binding protein 43 (TDP-43) is a DNA/RNA binding playing critical role in the regulation of transcription, splicing and RNA stability. Mutations <ns4:italic>TARDBP </ns4:italic>leading to aggregation, are suspected be characteristic feature various neurogenerative diseases. The lack well-characterized anti- TDP-43 antibodies acts as barrier establish reproducible research. In this study, we characterized eighteen commercial for Western blot, immunoprecipitation,...
<ns4:p>TAR DNA-binding protein 43 (TDP-43) is a DNA/RNA binding playing critical role in the regulation of transcription, splicing and RNA stability. Mutations <ns4:italic>TARDBP </ns4:italic>leading to aggregation, are suspected be characteristic feature various neurogenerative diseases. The lack well-characterized anti- TDP-43 antibodies acts as barrier establish reproducible research. In this study, we characterized eighteen commercial for Western blot, immunoprecipitation,...
CHCHD10 is a mitochondrial protein, implicated in the regulation of morphology and cristae structure, as well maintenance DNA integrity. Recently discovered to be associated with amyotrophic lateral sclerosis (ALS) frontotemporal dementia (FTD) its mutant form, scientific community would benefit from availability validated anti-CHCHD10 antibodies. In this study, we characterized four commercial antibodies for Western Blot, immunoprecipitation, immunofluorescence using standardized...
<ns5:p>Moesin is a cytoskeletal adaptor protein, involved in the modification of actin cytoskeleton, with relevance to Alzheimer’s Disease. Well characterized anti-Moesin antibodies would benefit scientific community. In this study, we ten commercial for Moesin Western Blot, immunoprecipitation, and immunofluorescence using standardized experimental protocol based on comparing read-outs knockout cell lines isogenic parental controls. We identified well-performing encourage readers use report...
RNA-binding protein Fused-in Sarcoma (FUS) plays an essential role in various cellular processes. Mutations the C-terminal domain region, where nuclear localization signal (NLS) is located, causes redistribution of FUS from nucleus to cytoplasm. In neurons, neurotoxic aggregates are formed as a result, contributing neurogenerative diseases. Well-characterized anti-FUS antibodies would enable reproducibility research, thereby benefiting scientific community.