A5063878177- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
- Ion Channels and Receptors
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- RNA and protein synthesis mechanisms
- Pleural and Pulmonary Diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Long-Term Effects of COVID-19
- Biosimilars and Bioanalytical Methods
- Lung Cancer Diagnosis and Treatment
- Biomedical and Engineering Education
- COVID-19 Clinical Research Studies
- Respiratory Support and Mechanisms
- Protein purification and stability
- Occupational exposure and asthma
- Macrophage Migration Inhibitory Factor
- Advanced Proteomics Techniques and Applications
- Bone fractures and treatments
- vaccines and immunoinformatics approaches
- Postharvest Quality and Shelf Life Management
- Occupational and environmental lung diseases
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Youth Substance Use and School Attendance
- Cellular transport and secretion
- Peptidase Inhibition and Analysis
University of Leicester
2016-2024
NIHR Leicester Biomedical Research Centre
2019-2024
Developmental Studies Hybridoma Bank
2024
University of Toronto
2024
McGill University
2024
Cell Signaling Technology (United States)
2024
Emory University
2024
NIHR Leicester Respiratory Biomedical Research Unit
2023
Glenfield Hospital
2022-2023
Kettering General Hospital
2022
Study objectives:“Sleep quality” is poorly defined yet ubiquitously used by researchers, clinicians and patients. While poor sleep quality a key feature of insomnia, there are few empirical investigations in insomnia Accordingly, our aim was to investigate the subjective meaning among individuals with normal sleepers.
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of problem remains largely anecdotal, as such, feasibility goal at least one potent specific antibody targeting each protein in a proteome cannot be assessed. Focusing for human proteins, we have scaled standardized characterization approach using parental knockout cell lines (Laflamme et al.,...
YCharOS is a collaborative initiative aimed at characterising antibodies against the entire human proteome. As of August 2023, they have presented comprehensive knockout characterisation data for 812 and 78 proteins using techniques such as Western blot, immunoprecipitation, immunofluorescence. consolidates its into reports (one protein per report) available on Zenodo, public repository controlled by CERN, to ensure open access. To enhance visibility their work, group progressively...
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of problem remains largely anecdotal, as such, feasibility goal at least one potent specific antibody targeting each protein in a proteome cannot be assessed. Focusing for human proteins, we have scaled standardized characterization approach using parental knockout cell lines (Laflamme et al.,...
Abstract Antibody-based research applications are critical for biological discovery. Yet, there no industry standards to compare the performance of antibodies in various applications. We describe a knockout cell line-based antibody characterization platform, developed, and approved jointly by academic researchers that enables systematic comparison western blot, immunoprecipitation, immunofluorescence procedures. The scalable protocols consist (i) identification appropriate lines studies,...
The transient receptor potential cation channel family member ankyrin 1 (TRPA1) is a target for several diseases, but detection of human TRPA1 (hTRPA1) protein in cells and tissues problematic as rigorous antibody validation lacking. We expressed hTRPA1 TRPA1-negative cell line to evaluate 5 commercially available antibodies by western blotting, immunofluorescence, immunocytochemistry flow cytometry. three most cited anti-TRPA1 lacked sensitivity and/or specificity, two mouse monoclonal...
Antibodies are one of the most important reagents used in biomedical and fundamental research, to identify, quantify proteins, contribute knowledge disease mechanisms, validate drug targets. Yet many antibodies research do not recognize their intended target, or additional molecules, compromising integrity findings leading waste resources, lack reproducibility, failure projects, delays development. Researchers frequently use without confirming that they perform as application interest. Here...
A single event can completely change the direction of a career in science; four researchers share their stories.
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of problem remains largely anecdotal, as such, feasibility goal at least one potent specific antibody targeting each protein in a proteome cannot be assessed. Focusing for human proteins, we have scaled standardized characterization approach using parental knockout cell lines (Laflamme et al.,...
TGFβ1-mediated myofibroblast activation contributes to pathological fibrosis in many diseases including idiopathic pulmonary (IPF), where resistance oxidant-mediated apoptosis is also evident. We therefore investigated the involvement of redox-sensitive TRPA1 ion channels on human lung myofibroblasts (HLMFs) cell death and pro-fibrotic responses.The effects TGFβ1 stimulation expression viability was studied HLMFs derived from IPF patients non-fibrotic patients. examined a model...
Idiopathic pulmonary fibrosis (IPF) is a chronic lung condition that more prevalent in males than females. The reasons for this are not fully understood, with differing environmental exposures due to historically sex-biased occupations, or diagnostic bias, being possible explanations. To date, over 20 independent genetic variants have been identified be associated IPF susceptibility, but these discovered when combining and Our aim was test the presence of sex-specific associations...
Antibodies are critical reagents to detect and characterize proteins. It is commonly understood that many commercial antibodies do not recognize their intended targets, but information on the scope of problem remains largely anecdotal, as such, feasibility goal at least one potent specific antibody targeting each protein in a proteome cannot be assessed. Focusing for human proteins, we have scaled standardized characterization approach using parental knockout cell lines (Laflamme et al.,...
Synaptotagmin-1 is a synaptic vesicle transmembrane protein that senses calcium influx via its tandem C2-domains, triggering synchronous neurotransmitter release. Disruption to
<ns4:p>RNA-binding protein Fused-in Sarcoma (FUS) plays an essential role in various cellular processes. Mutations the C-terminal domain region, where nuclear localization signal (NLS) is located, causes redistribution of FUS from nucleus to cytoplasm. In neurons, neurotoxic aggregates are formed as a result, contributing neurogenerative diseases. Well-characterized anti-FUS antibodies would enable reproducibility research, thereby benefiting scientific community. this study, we...
<b>Introduction:</b> Idiopathic pulmonary fibrosis (IPF) is a progressive disease of unknown aetiology. TGFβ1 key cytokine driving pro-fibrotic myofibroblast activity. Ca<sup>2+</sup> signals are critical in driven functions and the permeable transient receptor potential (TRP)A1 channel implicated TGFβ1-driven responses. The role TRPA1 human lung unknown. <b>Methods:</b> Human myofibroblasts (HLMFs) were derived from non-fibrotic (NF) IPF tissue. TRP expression function examined using...
<b>Background:</b> Idiopathic pulmonary fibrosis (IPF) is driven by failure of the normal wound healing response to tissue injury, leading inappropriate accumulation human lung myofibroblasts (HLMF) and development fibroblastic foci. Targeting HLMF apoptosis in IPF emerging as a therapeutic strategy. TGFβ1 key pro-fibrotic cytokine driving IPF, has dual roles both promoting inhibiting apoptosis. Redox-sensitive transient receptor potential (TRP) ion channels are implicated regulation cell...
Background: Severe COVID-19 pneumonitis in elderly frail patients is associated with poor outcomes, and therefore invasive mechanical ventilation often deemed an inappropriate course of action. Some evidence suggests high-flow nasal oxygen (HFNO) may prevent the need for other groups patients, but whether it appropriate ceiling care older unknown. Methods: We retrospectively identified severe requiring FiO 2 >60% who were or non-invasive continuous positive airway pressure (CPAP). Our...