A5078243091- Diabetes and associated disorders
- Pancreatic function and diabetes
- Alzheimer's disease research and treatments
- Neurobiology and Insect Physiology Research
- Genetic Associations and Epidemiology
- RNA Interference and Gene Delivery
- Lipid metabolism and biosynthesis
- Cellular transport and secretion
- Genetics, Aging, and Longevity in Model Organisms
- Monoclonal and Polyclonal Antibodies Research
- Autophagy in Disease and Therapy
- Neuroscience and Neuropharmacology Research
- Adipose Tissue and Metabolism
- Extracellular vesicles in disease
- T-cell and B-cell Immunology
- S100 Proteins and Annexins
- Child Nutrition and Feeding Issues
- Biosimilars and Bioanalytical Methods
- Advanced Proteomics Techniques and Applications
- Birth, Development, and Health
- MicroRNA in disease regulation
- Gestational Diabetes Research and Management
- Infant Development and Preterm Care
- Ubiquitin and proteasome pathways
- Nuclear Receptors and Signaling
University of Oxford
2018-2024
Centre for Human Genetics
2018-2024
The Francis Crick Institute
2017-2021
Constructor University
2009
Article28 July 2020Open Access Source DataTransparent process Glutamine deprivation alters the origin and function of cancer cell exosomes Shih-Jung Fan Department Physiology, Anatomy Genetics, University Oxford, UK Search for more papers by this author Benjamin Kroeger orcid.org/0000-0003-1479-3841 Pauline P Marie Esther M Bridges Oncology, Weatherall Institute Molecular Medicine, John D Mason Kristie McCormick Christos E Zois Helen Sheldon Nasullah Khalid Alham Biomedical Engineering,...
Early-life nourishment exerts long-term influences upon adult physiology and disease risk. These lasting effects of diet are well established but the underlying mechanisms only partially understood. Here we show that restricting dietary yeast during Drosophila development can, depending subsequent environment, more than double median lifespan. Developmental acts via a influence production toxic molecules, which term autotoxins, shed into environment shorten lifespan both sexes. Autotoxins...
Alzheimer's disease (AD) is an age-related neurodegenerative condition and the most common type of dementia, characterised by pathological accumulation extracellular plaques intracellular neurofibrillary tangles that mainly consist amyloid-β (Aβ) hyperphosphorylated tau aggregates, respectively. Previous studies in mouse models with a targeted knock-out microtubule-associated protein (Mapt) gene demonstrated Aβ-driven toxicity tau-dependent. However, human cellular chronic lowering remain...
Antibodies are essential research tools whose performance directly impacts conclusions and reproducibility. Owing to its central role in Alzheimer's disease other dementias, hundreds of distinct antibody clones have been developed against the microtubule-associated protein Tau multiple proteoforms. Despite this breadth offer, limited understanding their poor selectivity hindered progress. Here, we validate a large panel antibodies by Western blot (79 reagents) immunohistochemistry (35...
Obesity-related renal lipotoxicity and chronic kidney disease (CKD) are prevalent pathologies with complex aetiologies. One hallmark of is the ectopic accumulation lipid droplets in podocytes proximal tubule cells. Renal observed human CKD patients high-fat diet (HFD) rodent models, but their precise role remains unclear. Here, we establish a HFD model Drosophila that recapitulates several other aspects mammalian CKD. Cell type–specific genetic manipulations show can overflow from adipose...
Given the potential shared aetiology between type 1 and 2 diabetes, we aimed to identify any genetic regions associated with both diseases. For associations where there is a signal allele that increases risk one disease also other, inference about could be made, develop therapeutic strategies treat or prevent diseases simultaneously. Alternatively, if co-localises divergent effect directions, it provide valuable biological insight into how association affects two differently.
Abstract Background The microtubule-associated protein Tau has attracted diverse and increasing research interest, with being mentioned in the title/abstract of nearly 34,000 PubMed-indexed publications to date. To accelerate studies into biology, characterisation its multiple proteoforms, including disease-relevant post-translational modifications (PTMs), role neurodegeneration, a multitude Tau-targeting antibodies have been developed, hundreds distinct antibody clones currently available...
Antibodies are one of the most important reagents used in biomedical and fundamental research, to identify, quantify proteins, contribute knowledge disease mechanisms, validate drug targets. Yet many antibodies research do not recognize their intended target, or additional molecules, compromising integrity findings leading waste resources, lack reproducibility, failure projects, delays development. Researchers frequently use without confirming that they perform as application interest. Here...
Current evidence has associated caspase activation with the regulation of basic cellular functions without causing apoptosis. Malfunction non-apoptotic activities may contribute to specific neurological disorders, metabolic diseases, autoimmune conditions and cancers. However, our understanding remains limited. Here, we show that prevents intracellular accumulation Patched receptor in autophagosomes subsequent Patched-dependent induction autophagy Drosophila follicular stem cells. These...
Secretory cells in glands and the nervous system frequently package store proteins destined for regulated secretion dense-core granules (DCGs), which disperse when released from cell surface. Despite relevance of this dynamic process to diseases such as diabetes human neurodegenerative disorders, our mechanistic understanding is relatively limited, because lack good models follow nanoscale events involved. Here, we employ prostate-like secondary (SCs) Drosophila male accessory gland dissect...
An in vivo model was used to investigate the role of cathepsins mouse intestine after mechanical manipulation. Inspection different segments by immunofluorescence microscopy provided evidence for a local release cathepsin B from cells individual gut sections shortly traumatic injury. Densitometry immunoblots ruled out alterations expression levels. Because similar results were obtained with both and rat trauma models, we interested identifying potential targets released early post-traumatic...
SUMMARY Exosomes are secreted extracellular vesicles (EVs) carrying diverse cargos, which can modulate recipient cell behaviour. They thought to derive from intraluminal formed in late endosomal multivesicular bodies (MVBs). An alternate exosome formation mechanism, is conserved fly human, described here, with exosomes unique including the GTPase Rab11, generated Rab11-positive recycling MVBs. Release of these cancer cells increased by reducing Akt/mechanistic Target Rapamycin (mTORC1)...
Insulin secretion increases progressively during pregnancy to maintain normal maternal blood glucose levels. The placenta plays a crucial role in this process by releasing hormones and extracellular vesicles into the circulation, which drive significant changes physiology. Placental vesicles, are detectable plasma of pregnant women, have been shown signal peripheral tissues contribute pregnancy-related conditions. While studies using murine models demonstrated that can modulate insulin...
Abstract Secretory cells in glands and the nervous system frequently package store proteins destined for regulated secretion dense-core granules (DCGs), which disperse when released from cell surface. Despite relevance of this dynamic process to diseases such as diabetes human neurodegenerative disorders, our mechanistic understanding is relatively limited, because lack good models follow nanoscale events involved. Here, we employ prostate-like secondary (SCs) Drosophila male accessory gland...
Abstract Obesity-related renal lipotoxicity and chronic kidney disease (CKD) are prevalent pathologies with complex aetiologies. One hallmark of is the ectopic accumulation lipid droplets in podocytes proximal tubule cells. Renal observed human CKD patients high-fat diet rodent models but their precise role remains unclear. Here, we establish a model Drosophila that recapitulates several other aspects mammalian CKD. Cell-type specific genetic manipulations show can overflow from adipose...
Abstract Aims/hypothesis Given the potential shared aetiology between type 1 and 2 diabetes, we aimed to identify any genetic regions associated with both diseases. For associations where there is a signal allele that increases risk one disease also other, inference about could be made, develop therapeutic strategies treat or prevent diseases simultaneously. Alternatively, if colocalises divergent effect directions, it provide valuable biological insight into how association affects two...
Abstract Background Alzheimer’s disease (AD) is an age-related neurodegenerative condition and the most common type of dementia, characterised by pathological accumulation extracellular plaques intracellular neurofibrillary tangles that mainly consist amyloid-β (Aβ) hyperphosphorylated tau aggregates, respectively. Previous studies in mouse models with a targeted knock-out microtubule-associated protein (Mapt) gene demonstrated Aβ-driven toxicity tau-dependent. However, human cellular...
Diabetologia. 2021 Jun;64(6):13421347. 10.1007/s00125-021-05428-0. https://pubmed.ncbi.nlm.nih.gov/33830302/