A5041755210- Genomics and Phylogenetic Studies
- Coccidia and coccidiosis research
- Protist diversity and phylogeny
- Parasitic Infections and Diagnostics
- Microbial Community Ecology and Physiology
- Malaria Research and Control
- Mosquito-borne diseases and control
- Helminth infection and control
- Adipose Tissue and Metabolism
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Pancreatic function and diabetes
- Toxoplasma gondii Research Studies
- Lipid metabolism and biosynthesis
- Geriatric Care and Nursing Homes
- Biomedical Ethics and Regulation
- Photosynthetic Processes and Mechanisms
- Family and Patient Care in Intensive Care Units
- Intensive Care Unit Cognitive Disorders
- Cellular transport and secretion
- COVID-19 epidemiological studies
- Pediatric Urology and Nephrology Studies
- Animal Nutrition and Physiology
- Adrenal and Paraganglionic Tumors
- Cancer, Hypoxia, and Metabolism
Oxford Brookes University
2019-2023
Royal Veterinary College
2019-2023
University of London
2019-2023
The Francis Crick Institute
2019-2021
University College London Hospitals NHS Foundation Trust
2020
University College London
2020
National Hospital for Neurology and Neurosurgery
2020
Photosynthetic euglenids are major contributors to fresh water ecosystems. Euglena gracilis in particular has noted metabolic flexibility, reflected by an ability thrive a range of harsh environments. E. been popular model organism and considerable biotechnological interest, but the absence gene catalogue hampered both basic research translational efforts. We report detailed transcriptome partial genome for Z1. The nuclear is estimated be around 500 Mb size, encodes over 36,000 proteins...
Abstract The size of the membrane-bound nucleus scales with cell in a wide range types but mechanisms determining overall nuclear remain largely unknown. Here we investigate role fission yeast inner membrane proteins size, and propose that Lap2-Emerin-Man1 domain protein Lem2 acts as barrier to flow between other parts cellular system. deletion increases into out envelope response changes synthesis nucleocytoplasmic transport, altering size. endoplasmic reticulum Lnp1 secondary flow,...
Hypoxia-inducible factor (HIF) is strikingly upregulated in many types of cancer, and there great interest applying inhibitors HIF as anticancer therapeutics. The most advanced these are small molecules that target the HIF-2 isoform through binding PAS-B domain HIF-2α. These undergoing clinical trials with promising results renal other cancers where considered to be driving growth. Nevertheless, a central question remains whether such affect physiological responses hypoxia at relevant doses....
Obesity-related renal lipotoxicity and chronic kidney disease (CKD) are prevalent pathologies with complex aetiologies. One hallmark of is the ectopic accumulation lipid droplets in podocytes proximal tubule cells. Renal observed human CKD patients high-fat diet (HFD) rodent models, but their precise role remains unclear. Here, we establish a HFD model Drosophila that recapitulates several other aspects mammalian CKD. Cell type–specific genetic manipulations show can overflow from adipose...
Introduced about a century ago, suramin remains frontline drug for the management of early-stage East African trypanosomiasis (sleeping sickness). Cellular entry into causative agent, protozoan parasite Trypanosoma brucei, occurs through receptor-mediated endocytosis involving parasite's invariant surface glycoprotein 75 (ISG75), followed by transport cytosol via lysosomal transporter. The molecular basis trypanocidal activity unclear, but some evidence suggests broad, specific, impacts on...
Significance During blood-stage development, the malaria parasite replicates inside erythrocytes of vertebrate host, where it engulfs and digests most available hemoglobin. This results in release oxygen-binding prosthetic group heme, which is highly toxic its unbound form. The crystallizes heme into an insoluble pigment called hemozoin, a process that vital for survival exploited antimalarial therapy. We demonstrate uses protein PV5 hemozoin formation interfering with expression can...
In vitro development of the complete life cycle Eimeria species has been achieved in primary cultures avian epithelial cells with low efficiency. The use immortalised cell lines simplifies procedures but only allows partial through one round parasite invasion and intracellular replication. We have assessed suitability Madin-Darby Bovine Kidney (MDBK) to support quantitative studies on sporozoite (first-generation schizogony merozoite production) tenella. Transmission electron microscopy...
The apical complex of apicomplexan parasites is essential for host cell invasion and intracellular survival as the site regulated exocytosis from specialised secretory organelles called rhoptries micronemes. Despite its importance, there are few data on three-dimensional organisation quantification these within or how they trafficked to this region plasma membrane exocytosis. In coccidian apicomplexans an additional tubulin-containing hollow barrel structure, conoid, which provides a...
Abstract The apical complex of apicomplexan parasites is essential for host cell invasion and intracellular survival as the site regulated exocytosis from specialised secretory organelles called rhoptries micronemes. Despite its importance, there little data on three-dimensional organisation quantification these within or how they are trafficked to this region plasma membrane exocytosis. In coccidian apicomplexans an additional tubulin-containing hollow barrel structure, conoid, which...
This protocol details the fixation of 1 mm diameter human kidney needle core biopsies in clinic, compatible with ultrastructural and molecular preservation for downstream clinical research applications, including those that use high resolution imaging light, X-ray electron microscopes.
Refractile bodies (RB) are large membrane-less organelles (MLO) of unknown function found as a prominent mismatched pair within the sporozoite stages all species Eimeria, parasitic coccidian protozoa.High resolution imaging methods including time-lapse live confocal microscopy and serial block face-scanning electron (SBF-SEM) were used to investigate morphology RB other intracellular before after invasion host cells.Live cell MDBK cells infected with E. tenella sporozoites confirmed previous...
Abstract Obesity-related renal lipotoxicity and chronic kidney disease (CKD) are prevalent pathologies with complex aetiologies. One hallmark of is the ectopic accumulation lipid droplets in podocytes proximal tubule cells. Renal observed human CKD patients high-fat diet rodent models but their precise role remains unclear. Here, we establish a model Drosophila that recapitulates several other aspects mammalian CKD. Cell-type specific genetic manipulations show can overflow from adipose...
ABSTRACT During blood stage development, malaria parasites are challenged with the detoxification of enormous amounts haem released during proteolytic catabolism erythrocytic haemoglobin. They tackle this problem by sequestering into bioinert crystals known as haemozoin. The mechanisms underlying biomineralization process remain enigmatic. Here, we demonstrate that both rodent and human parasite species secrete internalize a lipocalin-like protein, PV5, to control crystallization....