A5073175338- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Biochemical and Molecular Research
- Synthesis and Biological Evaluation
- Metabolomics and Mass Spectrometry Studies
- Parasites and Host Interactions
- HIV/AIDS drug development and treatment
- Lysosomal Storage Disorders Research
- Malaria Research and Control
- Urban Stormwater Management Solutions
- Polyamine Metabolism and Applications
- Microbial Metabolic Engineering and Bioproduction
- Carbohydrate Chemistry and Synthesis
- Analytical Chemistry and Chromatography
- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Bioinformatics and Genomic Networks
- Mosquito-borne diseases and control
- Biosensors and Analytical Detection
- Biochemical Acid Research Studies
- Insect symbiosis and bacterial influences
- Adenosine and Purinergic Signaling
- Calcium signaling and nucleotide metabolism
- Mass Spectrometry Techniques and Applications
- Enzyme Structure and Function
University of Glasgow
2016-2025
Wellcome Centre for Molecular Parasitology
2015-2024
Wellcome Trust
2010-2024
Microsoft (Ireland)
2022
James Cook University
2022
Dalhousie University
2021
Lincoln Electric (United States)
2020
Addenbrooke's Hospital
2017-2019
Institute of Infection and Immunity
2016-2019
Mylan (Switzerland)
2019
Metabolomics is an emerging field of postgenomic biology concerned with comprehensive analysis small molecules in biological systems. However, difficulties associated the identification detected metabolites currently limit its application. Here we demonstrate that a retention time prediction model can improve metabolite on hydrophilic interaction chromatography (HILIC)–high-resolution mass spectrometry metabolomics platform. A quantitative structure relationship (QSRR) model, incorporating...
Significance Tandem MS is a technique for compound identification in untargeted metabolomics experiments. Because of lack reference spectra, most molecules cannot be identified, and many spectra used. We present MS2LDA, an unsupervised method (inspired by text-mining) that extracts common patterns mass fragments neutral losses—Mass2Motifs—from collections fragmentation spectra. Structurally characterized Mass2Motifs can used to annotate which no exist expose biochemical relationships between...
Abstract Summary: The application of emerging metabolomics technologies to the comprehensive investigation cellular biochemistry has been limited by bottlenecks in data processing, particularly noise filtering and metabolite identification. IDEOM provides a user-friendly processing that automates identification peaks, paying particular attention common sources false identifications generated liquid chromatography–mass spectrometry (LC–MS) platforms. Building on advanced tools such as mzMatch...
Proline metabolism has been studied in procyclic form Trypanosoma brucei. These parasites consume six times more proline from the medium when glucose is limiting supply than this carbohydrate present as an abundant energy source. The sensitivity of T. brucei to oligomycin increases by three orders magnitude are obliged catabolize depleted glucose. This indicates that oxidative phosphorylation far important latter case available and needs parasite can be fulfilled substrate level alone. A...
We present the use of a simple microfluidic technique to separate living parasites from human blood. Parasitic trypanosomatids cause range and animal diseases. African trypanosomes, responsible for trypanosomiasis (sleeping sickness), live free in blood other tissue fluids. Diagnosis relies on detection due their often low numbers against an overwhelming background predominantly red cells it is crucial By modifying method deterministic lateral displacement, confining channels optimized depth...
ABSTRACT Sleeping sickness, caused by Trypanosoma brucei spp., has become resurgent in sub-Saharan Africa. Moreover, there is an alarming increase treatment failures with melarsoprol, the principal agent used against late-stage sleeping sickness. In T. , uptake of melarsoprol as well diamidines thought to be mediated P2 aminopurine transporter, and loss function been implicated resistance these agents. The trypanosomal gene TbAT1 found encode a P2-type transporter when expressed yeast. Here...
High-throughput metabolomic experiments aim at identifying and ultimately quantifying all metabolites present in biological systems. The are interconnected through metabolic reactions, generally grouped into pathways. Classical maps provide a relational context to help interpret metabolomics wide range of tools have been developed place within However, the representation separate disconnected pathways overlooks most connectivity metabolome. By definition, reference cannot integrate novel nor...
Human African trypanosomiasis, endemic to sub-Saharan Africa, is invariably fatal if untreated. Its causative agent the protozoan parasite Trypanosoma brucei. Eflornithine used as a first line treatment for human but there risk that resistance could thwart its use, even when in combination therapy with nifurtimox. resistant trypanosomes were selected vitro and subjected biochemical genetic analysis. The phenotype was verified vivo. Here we report molecular basis of resistance. While drug's...
In this study, we assessed three liquid chromatographic platforms: reversed phase (RP), aqueous normal (ANP), and hydrophilic interaction (HILIC) for the analysis of polar metabolite standard mixtures their coverage urinary metabolites. The two zwitterionic HILIC columns showed high-quality performance standards, improved separation isomers, greatest metabolites in urine. contrast, on column, most eluted very rapidly with little or no separation. Using an Exactive Orbitrap mass spectrometer...
African trypanosomes cause sleeping sickness in humans, a disease that is typically fatal without chemotherapy. Unfortunately, drug resistance common and melarsoprol-resistant often display cross-resistance to pentamidine. Although melarsoprol/pentamidine (MPXR) has been an area of intense interest for several decades, our understanding the underlying mechanisms remains incomplete. Recently, locus encoding two closely related aquaglyceroporins, AQP2 AQP3, was linked MPXR high-throughput...
While the apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii are thought to primarily depend on glycolysis for ATP synthesis, recent studies have shown that they can fully catabolize glucose in a canonical TCA cycle. However, these lack mitochondrial isoform of pyruvate dehydrogenase identity enzyme catalyses conversion acetyl-CoA remains enigmatic. Here we demonstrate branched chain ketoacid (BCKDH) complex is missing link, functionally replacing PDH both T. P. berghei....