A5054153619- Parasitic Infections and Diagnostics
- Toxoplasma gondii Research Studies
- Plant Disease Resistance and Genetics
- Plant and Fungal Interactions Research
- Genomics and Phylogenetic Studies
- Coccidia and coccidiosis research
- Insect symbiosis and bacterial influences
- Trypanosoma species research and implications
- Chromosomal and Genetic Variations
- Enterobacteriaceae and Cronobacter Research
- RNA modifications and cancer
- Mosquito-borne diseases and control
- Vector-borne infectious diseases
- Protist diversity and phylogeny
- Plant Virus Research Studies
- Calpain Protease Function and Regulation
- Biochemical Acid Research Studies
- Cytomegalovirus and herpesvirus research
- Parasite Biology and Host Interactions
- Aquaculture disease management and microbiota
Université Clermont Auvergne
2005-2024
Laboratoire Microorganismes Génome et Environnement
2006-2024
Centre National de la Recherche Scientifique
2005-2024
Clermont Université
2010-2017
University of Geneva
2008-2014
While the apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii are thought to primarily depend on glycolysis for ATP synthesis, recent studies have shown that they can fully catabolize glucose in a canonical TCA cycle. However, these lack mitochondrial isoform of pyruvate dehydrogenase identity enzyme catalyses conversion acetyl-CoA remains enigmatic. Here we demonstrate branched chain ketoacid (BCKDH) complex is missing link, functionally replacing PDH both T. P. berghei....
The glideosome is an actomyosin-based machinery that powers motility in Apicomplexa and participates host cell invasion egress from infected cells. central component of the glideosome, myosin A (MyoA), a motor recruited at pellicle by acylated gliding-associated protein GAP45. In Toxoplasma gondii, GAP45 also contributes to cohesion pellicle, composed inner membrane complex (IMC) plasma membrane, during traction. GAP70 was previously identified as paralog tailored recruit MyoA apical cap...
Microsporidia have been identified as pathogens that important effects on our health, food security and economy. A key to the success of these obligate intracellular is their unique invasion organelle, polar tube, which delivers nucleus containing sporoplasm into host cells during invasion. Due size rapidity tube discharge passage, absence genetic techniques for manipulation microsporidia, study this organelle has difficult there relatively little known regarding formation function proteins...
Microsporidian genomes are the leading models to understand streamlining in response a pathogenic lifestyle; they gene-poor and often possess small genomes. In this study, we show feature of microsporidian that contrasts pattern genome reduction. Specifically, investigations targeted at Anncaliia algerae, human pathogen with size 23 Mb, revealed presence hitherto undetected diversity transposable elements (TEs). A total 240 TE families per were identified, exceeding found many free-living...
Microsporidia are obligate intracellular parasites able to infect a wide range of hosts from invertebrates vertebrates. The success their invasion process is based on an original organelle, the polar tube, which suddenly extruded spore inoculate sporoplasm into host cytoplasm. tube mainly composed proteins named (PTPs). A comparative analysis allowed us identify genes coding for 5 PTPs (PTP1 PTP5) in genome microsporidian Anncaliia algerae. While PTP1 and PTP2 found whole PTP3 present large...
Toxoplasma gondii possesses 11 rather atypical myosin heavy chains. The only light chain described to date is MLC1, associated with A, and contributing gliding motility. In this study, we examined the repertoire of calmodulin-like proteins in Apicomplexans, identified six putative chains determined their subcellular localization T. Plasmodium falciparum. MLC2, found coccidians, D via its calmodulin (CaM)-like domain anchored plasma membrane N-terminal extension. Molecular modeling suggests...
The intestinal protistan parasite Blastocystis is characterized by an extensive genetic variability with 17 subtypes (ST1-ST17) described to date. Only the whole genome of a human ST7 isolate was previously sequenced. Here we report draft sequence ST4-WR1 isolated from laboratory rodent at Singapore.
With nearly 1700 species, Microsporidia represent a group of obligate intracellular eukaryotes with veterinary, economic and medical impacts. To help understand the biological functions these microorganisms, complete genome sequencing is routinely used. Nevertheless, proper prediction their gene catalogue challenging due to taxon-specific evolutionary features. As innovative annotation strategies are needed obtain representative snapshot overall lifestyle parasites, MicroAnnot tool,...
ABSTRACT Microsporidia are obligate intracellular fungus-related parasites considered as emerging opportunistic human pathogens. Their extracellular infective and resistance stage is a spore surrounded by unique plasma membrane protected thick cell wall consisting of two layers: the electron-lucent inner endospore which contains chitin protein components outer-electron-dense mainly proteinaceous exospore. We identified whole sequences proteins in microsporidian species Encephalitozoon hellem...
Microsporidia of the Encephalitozoon species are frequently found as opportunistic pathogens immunocompromised patients, but very little is known about prevalence and significance infection in immunocompetent individuals. It was reported previously that 8% Dutch blood donors 5% pregnant French women had an immunoglobulin G (IgG) immune response against specific organelles intestinalis. These organelles, so-called polar tube anchoring disk, used to penetrate membranes host cells during...
Microsporidia are ubiquitous intracellular pathogens whose opportunistic nature led to their increased recognition with the rise of AIDS pandemic. As RNA world was largely unexplored in this parasitic lineage, we developed a dedicated silico methodology carry out exhaustive identification ncRNAs across Encephalitozoon and Nosema genera. Thus, previously missing U1 small nuclear (snRNA) nucleolar RNAs (snoRNAs) targeting only LSU rRNA were highlighted further validated using 5' 3'RACE-PCR...
The proper prediction of the gene catalogue an organism is essential to obtain a representative snapshot its overall lifestyle, especially when it not amenable culturing. Microsporidia are obligate intracellular, sometimes hard culture, eukaryotic parasites known infect members every animal phylum. To date, sequencing and annotation microsporidian genomes have revealed poor complement with highly reduced sizes. In present paper, we investigated whether such sizes may induced biases for...
We present the draft genome sequence of Tubulinosema ratisbonensis, a microsporidium species infecting Drosophila melanogaster A total 3,013 protein-encoding genes and an array transposable elements were identified. This work represents necessary step to develop novel model host-parasite relationships using highly tractable genetic D. melanogaster.
With nearly 1,700 species, Microsporidia represent a group of obligate intracellular eukaryotes with veterinary, economic and medical impacts. To help in understanding the biological functions these microorganisms, complete genome sequencing is used routinely. Nevertheless, proper prediction their gene catalogue challenging due to taxon-specific evolutionary features. As innovative annotation strategies are needed obtain representative snapshot overall lifestyle parasites, MicroAnnot tool,...