A5033076657- Parasitic Infections and Diagnostics
- Coccidia and coccidiosis research
- Toxoplasma gondii Research Studies
- Veterinary medicine and infectious diseases
- Herpesvirus Infections and Treatments
- Animal Nutrition and Physiology
- Helminth infection and control
- Microbial infections and disease research
- Polyamine Metabolism and Applications
- Animal Disease Management and Epidemiology
- Insect and Pesticide Research
- Research on Leishmaniasis Studies
- vaccines and immunoinformatics approaches
- Vector-borne infectious diseases
- Hemoglobinopathies and Related Disorders
- Antimicrobial Peptides and Activities
- Essential Oils and Antimicrobial Activity
- RNA and protein synthesis mechanisms
- Pharmacological Effects and Assays
- Bacterial Genetics and Biotechnology
- Venomous Animal Envenomation and Studies
- Hepatitis B Virus Studies
- Pharmacological Effects of Natural Compounds
- Bird parasitology and diseases
- Aquaculture disease management and microbiota
Royal Veterinary College
2016-2024
University of London
2016-2024
Universidad Complutense de Madrid
2008-2016
This study investigated the in vitro effects of Greek oregano and garlic essential oils on inhibition Eimeria parasites their vivo production performance, intestinal bacteria counts oocyst output. An assay was performed using tenella Wisconsin strain sporozoites Madin-Darby bovine kidney (MDBK) cells. Intracellular sporozoite invasion quantified by detection E. DNA qPCR from cell monolayers harvested at 2 24 hours post-infection. Parasite inhibited oil concentration 100 ug/ml 83% or 93%...
Origanum vulgare subsp. hirtum, Thymus vulgaris, and Salvia fructicosa are aromatic plants commonly found in Mediterranean countries traditionally used Greece as a remedy for humans, since they well known potent antibacterial, antioxidant, anti-inflammatory agents. Essential oils (EOs) derived from cultivated the mountainous region of Epirus, Greece, were investigated their inhibitory activity against key microorganisms with relevance to avian health, while also assessing antioxidant...
Eimeria species are parasitic protozoa that cause coccidiosis, an intestinal disease commonly characterised by malabsorption, diarrhoea and haemorrhage is particularly important in chickens. Vaccination against chicken coccidiosis effective using wild-type or attenuated live parasite lines. The development of protocols to express foreign proteins has opened up the possibility vaccines deliver heterologous antigens function as multivalent vaccine vectors could protect chickens a range...
Abstract Eimeria is a genus of apicomplexan parasites that contains large number species, most which are absolutely host‐specific. Seven species have been recognized to infect chickens. Infection susceptible chickens results in an intestinal disease called coccidiosis, characterized by mucoid or hemorrhagic enteritis, associated with impaired feed conversion mortality severe cases. Intensive farming practices increased the significance coccidiosis since parasite transmission favored...
In vitro development of the complete life cycle Eimeria species has been achieved in primary cultures avian epithelial cells with low efficiency. The use immortalised cell lines simplifies procedures but only allows partial through one round parasite invasion and intracellular replication. We have assessed suitability Madin-Darby Bovine Kidney (MDBK) to support quantitative studies on sporozoite (first-generation schizogony merozoite production) tenella. Transmission electron microscopy...
Cheap, easy-to-produce oral vaccines are needed for control of coccidiosis in chickens to reduce the impact this disease on welfare and economic performance. Saccharomyces cerevisiae yeast expressing three Eimeria tenella antigens were developed delivered as heat-killed, freeze-dried whole four separate studies. After vaccination, E. replication was reduced following low dose challenge (250 oocysts) Hy-Line Brown layer (p<0.01). Similarly, caecal lesion score vaccinated using a mixture S....
Abstract Background Poultry coccidiosis is a parasitic enteric disease with highly negative impact on chicken production. In-feed chemoprophylaxis remains the primary method of control, but increasing ineffectiveness anticoccidial drugs, and potential future restrictions their use has encouraged commercial live vaccines. Availability such formulations constrained by production, which relies chickens. Several experimental approaches have been taken to explore ways reduce complexity cost...
Eimeria spp. are intracellular parasites that have a major impact on poultry. Effective live vaccines available and the development of reverse genetic technologies has raised prospect using as recombinant vectors to express additional immunoprotective antigens. To study ability secrete foreign antigens or display them surface sporozoite, transiently transfected populations E. tenella expressing fluorescent protein mCherry, linked endogenous signal peptide (SP)...
Identification of differentially expressed proteins during Neospora caninum tachyzoite-bradyzoite conversion processes may lead to a better knowledge the pathogenic mechanisms developed by this important parasite cattle. In present work, differential expression proteomic study tachyzoite and bradyzoite stages was accomplished for first time applying DIGE technology coupled with MS analysis. Up 72 spots were visualized (1.5-fold in relative abundance, p<0.05, t-test). A total 53 more abundant...
The apical complex of apicomplexan parasites is essential for host cell invasion and intracellular survival as the site regulated exocytosis from specialised secretory organelles called rhoptries micronemes. Despite its importance, there are few data on three-dimensional organisation quantification these within or how they trafficked to this region plasma membrane exocytosis. In coccidian apicomplexans an additional tubulin-containing hollow barrel structure, conoid, which provides a...
SUMMARY Here we present the detection of a gene cluster for Neospora caninum surface genes, similar to Toxoplasma gondii SRS9 locus, and cloning characterization Nc gene. PCR genome walking, using BSR4 as framework, allows identification, upstream , 2 sequences homologous SRS5 Ubiquinol-cytochrome C reductase genes and, downstream an ORF 1191 bp coding 396-amino acid polypeptide with 59% similarity TgSRS9 antigen. A putative 39-residue signal peptide was found at NH2-terminus followed by...