A5016590698- Single-cell and spatial transcriptomics
- Neuroinflammation and Neurodegeneration Mechanisms
- CRISPR and Genetic Engineering
- Neuroscience and Neuropharmacology Research
- Wildlife Ecology and Conservation
- Wildlife-Road Interactions and Conservation
- Alzheimer's disease research and treatments
- Virus-based gene therapy research
- Food Security and Health in Diverse Populations
- Advanced Proteomics Techniques and Applications
- RNA Research and Splicing
- Organic Food and Agriculture
- Tryptophan and brain disorders
- Rangeland and Wildlife Management
- Signaling Pathways in Disease
University of Oxford
2022-2024
Centre for Human Genetics
2023-2024
Antibodies are essential research tools whose performance directly impacts conclusions and reproducibility. Owing to its central role in Alzheimer's disease other dementias, hundreds of distinct antibody clones have been developed against the microtubule-associated protein Tau multiple proteoforms. Despite this breadth offer, limited understanding their poor selectivity hindered progress. Here, we validate a large panel antibodies by Western blot (79 reagents) immunohistochemistry (35...
Aberrant cortical development is a key feature of neurodevelopmental disorders such as autism spectrum disorder and schizophrenia. Both genetic environmental risk factors are thought to contribute defects in development; however, model systems that can capture the dynamic process human not well established. To address this challenge, we combined recent progress induced pluripotent stem cell differentiation with advanced live imaging techniques establish novel three-dimensional neurosphere...
Abstract Defective cortical development is responsible for neurodevelopmental disorders that manifest in childhood and adolescence. However, model systems can explore the effects of genetic environmental factors on human are not well established. Therefore, we developed a novel neurosphere assay combined recent progress induced pluripotent stem cell (iPSC) differentiation with advanced live imaging techniques to study key aspects development. We subsequently applied this investigate viral...
Abstract Genetic risk factors contribute to cortical developmental abnormalities observed in a range of neurodevelopmental disorders including autism and schizophrenia. Emerging evidence suggests that IL1RAPL1 is common gene across many disorders. We combined CRISPR editing with induced pluripotent stem cell derived neuronal microglia model systems study the function effects disease relevant deletion . investigated several key aspects human development ranging from radial glia extension,...