A5092958624- Cellular transport and secretion
- Monoclonal and Polyclonal Antibodies Research
- Endoplasmic Reticulum Stress and Disease
- Erythrocyte Function and Pathophysiology
- Ubiquitin and proteasome pathways
- Genetic Neurodegenerative Diseases
- Sphingolipid Metabolism and Signaling
- Viral Infectious Diseases and Gene Expression in Insects
- Pancreatic function and diabetes
- Glycosylation and Glycoproteins Research
- interferon and immune responses
- Advanced Biosensing Techniques and Applications
- RNA and protein synthesis mechanisms
- Wnt/β-catenin signaling in development and cancer
- Mitochondrial Function and Pathology
- Viral Infections and Vectors
- Genetics and Neurodevelopmental Disorders
- CAR-T cell therapy research
- Protein Structure and Dynamics
- Amyotrophic Lateral Sclerosis Research
- Lipid Membrane Structure and Behavior
- Peptidase Inhibition and Analysis
- Transgenic Plants and Applications
- Neuroscience and Neuropharmacology Research
- Calcium signaling and nucleotide metabolism
Montreal Neurological Institute and Hospital
2024-2025
McGill University
2024-2025
Université Laval
2023-2024
Centre hospitalier universitaire de Québec
2023-2024
Developmental Studies Hybridoma Bank
2024
University of Toronto
2024
University of Leicester
2024
Cell Signaling Technology (United States)
2024
Emory University
2024
Structural Genomics Consortium
2024
Abstract Antibody-based research applications are critical for biological discovery. Yet, there no industry standards to compare the performance of antibodies in various applications. We describe a knockout cell line-based antibody characterization platform, developed, and approved jointly by academic researchers that enables systematic comparison western blot, immunoprecipitation, immunofluorescence procedures. The scalable protocols consist (i) identification appropriate lines studies,...
<ns4:p>The enzyme stearoyl-CoA desaturase (SCD1) is a modulator of lipid metabolism by catalyzing the biosynthesis mono-unsaturated fatty acids from saturated acids. Understanding specific mechanisms which SCD1 plays in health and disease can provide novel insides therapeutic targets, process that would be facilitated availability high-quality antibodies. Here we have characterized nine commercial antibodies for western blot, immunoprecipitation, immunofluorescence using standardized...
<ns4:p>Huntingtin encodes a 3144 amino acid protein, with polyglutamine repeat tract at the N-terminus. Expansion of this above pathogenic threshold 36 repeats is causative mutation Huntington's disease, neurodegenerative disorder characterized by loss striatal neurons. Here we have twenty Huntingtin commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using standardized experimental protocol based on comparing read-outs in knockout cell lines isogenic parental...
<ns4:p>Stimulator of interferon genes protein (STING1) is an immune adaptor which promotes innate defense mechanisms against pathogens. To enhance our understanding STING1-associated disease, it essential to make high-performing antibodies accessible the scientific community. This study aims improve reliability STING1 research as we have characterized sixteen commercial for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing...
<ns3:p>TAF15 (TATA-box binding protein-associated factor 15) is a member of the FET protein family, known for their roles in transcriptional regulation and RNA metabolism. Here we have characterized five TAF15 commercial antibodies western blot, immunoprecipitation, immunofluorescence using standardized experimental protocol based on comparing read-outs knockout cell lines isogenic parental controls. These studies are part larger, collaborative initiative seeking to address antibody...
<ns4:p>Sphingosine 1-phosphate receptor 1 (S1PR1) is a G-coupled protein that induces crucial biological processes when bound by sphingosine 1-phosphate. Here, we have characterized nine S1PR1 commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using standardized experimental protocol based on comparing read-outs in knockout cell lines isogenic parental controls. These studies are part of larger, collaborative initiative seeking to address antibody...
<ns3:p>Rab1 is a highly conserved small GTPase that exists in humans as two isoforms: Rab1A and Rab1B, sharing 92% sequence identity. These proteins regulate vesicle trafficking between the endoplasmic reticulum (ER) Golgi within stacks. Rab1B may be oncogenes, they are frequently dysregulated various human cancers. Moreover, contribute to progression of Parkinson’s disease. The availability high-quality antibodies specific for or essential understand distinct functions these Rab1 both...
The subcellular distribution of the polarity protein Yurt (Yrt) is subjected to a spatio-temporal regulation in Drosophila melanogaster embryonic epithelia. After cellularization, Yrt binds lateral membrane ectodermal cells and maintains this localization throughout embryogenesis. During terminal differentiation epidermis, accumulates at septate junctions also recruited apical domain. Although mechanisms through which associates with domain have been deciphered, how remains as an outstanding...
CSNK1A1 is a key regulator of various signalling pathways, including the Wnt/β-catenin pathway. Playing central role in cellular function and disease pathology, has emerged as an attractive protein target for therapeutic development. In this study we characterize ten commercial antibodies western blot, immunoprecipitation, immunofluorescence using standardized experimental protocol based on comparing read-outs knockout cell lines isogenic parental controls. This part larger, collaborative...
Synaptotagmin-1 is a synaptic vesicle transmembrane protein that senses calcium influx via its tandem C2-domains, triggering synchronous neurotransmitter release. Disruption to
Protein-glutamine gamma-glutamyltransferase 2 (TGM2) is a Ca
<ns3:p>VAPB is an adaptor protein known for its role as anchor other proteins at the endoplasmic reticulum. A mutant form of VAPB has been linked to amyotrophic lateral sclerosis and underlying mechanisms resulting from this defect are studied by researchers in area uncover implication disease. Here we have characterized six commercial antibodies western blot, immunoprecipitation, immunofluorescence using a standardized experimental protocol based on comparing read-outs knockout cell lines...
<ns3:p>Protein phosphatase 2A is a serine/threonine with activity dependent on an associated regulatory subunit, serine/threonine-protein 56 kDa subunit delta (δ) isoform (PPP2R5D). PPP2R5D the δ in B56 family of subunits. Abundantly expressed brain and involved broad range cellular processes, plays essential role modulating key neuronal pathways signalling. Pathogenic mutations <ns3:italic>PPP2R5D</ns3:italic> gene are linked to clinical symptoms characterized by neurodevelopmental delay,...
<ns4:p>Phosphatidylinositol-specific phospholipase C gamma 2 (PLC-gamma-2) is an enzyme that regulates the function of immune cells. PLC-gamma-2 has been implicated in neurodegenerative and autoimmune disorders, yet investigation this protein limited by a lack independently characterized antibodies. Here we have eleven commercial antibodies for use Western Blot, immunoprecipitation, immunofluorescence using standardized experimental protocol based on comparing read-outs knockout cell lines...
<ns3:p>Protein-glutamine gamma-glutamyltransferase 2 (TGM2) is a Ca<ns3:sup>2+</ns3:sup> dependent enzyme that catalyzes transglutaminase cross-linking modifications. TGM2 involved in various diseases, either protective or contributory manner, making it crucial protein to study and determine its therapeutic potential. Identifying high-performing antibodies would facilitate these investigations. Here we have characterized seventeen commercial for western blot sixteen immunoprecipitation,...
<ns3:p>Sphingosine 1-phosphate receptor 1 (S1PR1) is a G-coupled protein that induces crucial biological processes when bound by sphingosine 1-phosphate. Here, we have characterized nine S1PR1 commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using standardized experimental protocol based on comparing read-outs in knockout cell lines isogenic parental controls. These studies are part of larger, collaborative initiative seeking to address antibody...
<ns3:p>Protein phosphatase 2A is a serine/threonine with activity dependent on an associated regulatory subunit, serine/threonine-protein 56 kDa subunit delta (δ) isoform (PPP2R5D). PPP2R5D the δ in B56 family of subunits. Abundantly expressed brain and involved broad range cellular processes, plays essential role modulating key neuronal pathways signalling. Pathogenic mutations <ns3:italic>PPP2R5D</ns3:italic> gene are linked to clinical symptoms characterized by neurodevelopmental delay,...
<ns3:p>Casein kinase II subunit alpha (CSNK2A1), a serine/threonine kinase, phosphorylates multiple protein substrates and is involved in diverse cellular biological processes. Implicated various human diseases, high-performing antibodies would help evaluate its potential as therapeutic target benefit the scientific community. In this study, we have characterized ten CSNK2A1 commercial for western blot, immunoprecipitation, immunofluorescence using standardized experimental protocol based on...
<ns3:p>Huntingtin encodes a 3144 amino acid protein, with polyglutamine repeat tract at the N-terminus. Expansion of this above pathogenic threshold 36 repeats is causative mutation Huntington's disease, neurodegenerative disorder characterized by loss striatal neurons. Here we have twenty Huntingtin commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using standardized experimental protocol based on comparing read-outs in knockout cell lines isogenic parental...
<ns4:p>Sphingosine 1-phosphate receptor 1 (S1PR1) is a G-coupled protein that induces crucial biological processes when bound by sphingosine 1-phosphate. Here, we have characterized nine S1PR1 commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using standardized experimental protocol based on comparing read-outs in knockout cell lines isogenic parental controls. These studies are part of larger, collaborative initiative seeking to address antibody...
<ns3:p>Casein kinase II subunit alpha (CSNK2A1), a serine/threonine kinase, phosphorylates multiple protein substrates and is involved in diverse cellular biological processes. Implicated various human diseases, high-performing antibodies would help evaluate its potential as therapeutic target benefit the scientific community. In this study, we have characterized ten CSNK2A1 commercial for western blot, immunoprecipitation, immunofluorescence using standardized experimental protocol based on...
<ns3:p>STING1 is an immune adaptor protein which promotes innate defense mechanisms against pathogens. Its role in modulating inflammation links STING1 to various pathologic conditions, positioning it as a key target for therapeutic interventions aimed at regulating responses. To advance our understanding of STING1-associated diseases, essential make high-performing antibodies readily accessible the scientific community. This study aims improve reliability research we have characterized...
<ns3:p>Rab10 is a small GTPase involved in cargo transport from the trans-Golgi network to plasma membrane and endocytic recycling back cell membrane. It has garnered significant interest neurodegenerative disease research, particularly due its phosphorylation by LRRK2 kinase. This relationship underscores importance of Rab10 cellular processes related pathology, specifically Parkinson’s disease. The accessibility renewable selective antibodies against would advance research efforts,...
<ns3:p>ADNP is a multifunctional protein involved in chromatin remodeling, transcription, and microtubule interaction, playing critical role brain development, with mutations linked to <ns3:italic>ADNP</ns3:italic>-Related Disorder. Here we have characterized seven ADNP commercial antibodies for western blot, immunoprecipitation, immunofluorescence using standardized experimental protocol based on comparing read-outs knockout cell lines isogenic parental controls. These studies are part of...