A5100685137- Cellular Mechanics and Interactions
- Optical Imaging and Spectroscopy Techniques
- Force Microscopy Techniques and Applications
- 3D Printing in Biomedical Research
- Blood properties and coagulation
- Photoacoustic and Ultrasonic Imaging
- Biocrusts and Microbial Ecology
- Infrared Thermography in Medicine
- Cancer Cells and Metastasis
- Cardiac electrophysiology and arrhythmias
- Laser Applications in Dentistry and Medicine
- Elasticity and Material Modeling
- Rheology and Fluid Dynamics Studies
- Collagen: Extraction and Characterization
- Cardiac Arrhythmias and Treatments
- Microtubule and mitosis dynamics
- Traumatic Brain Injury and Neurovascular Disturbances
- Radiation Dose and Imaging
- Developmental Biology and Gene Regulation
- Neuroscience and Neural Engineering
- Nerve injury and regeneration
- Microfluidic and Bio-sensing Technologies
- Cardiovascular Effects of Exercise
- Tendon Structure and Treatment
- Photoreceptor and optogenetics research
Consejo Nacional de Investigaciones Científicas y Técnicas
2024
Institute of Photonic Sciences
2021-2023
Technical University of Munich
2008-2021
Barcelona Institute for Science and Technology
2021
Hospital Ramos Mejía
2018
Pennsylvania State University
2014
University of Bayreuth
2006-2008
Centre de Biologie Structurale
2006
Universidad de Navarra
2002
Abstract Epithelial branch elongation is a central developmental process during branching morphogenesis in diverse organs. This fundamental growth into large arborized epithelial networks accompanied by structural reorganization of the surrounding extracellular matrix (ECM), well beyond its mechanical linear response regime. Here, we report that ductal within human mammary organoid branches relies on non-linear and plastic collagen. Specifically, demonstrate collective back-and-forth motion...
Journal Article The compaction of gels by cells: a case collective mechanical activity Get access Pablo Fernandez, Fernandez Lehrstuhl für Zellbiophysik E27, Technische Universität München, James-Franck-Strasse 1, D-85748 Garching, Germany E-mail: pfernand@ph.tum.de Search for other works this author on: Oxford Academic Google Scholar Andreas R. Bausch abausch@ph.tum.de Integrative Biology, Volume Issue 3, March 2009, Pages 252–259, https://doi.org/10.1039/b822897c Published: 02 February...
We report a cylindrical-peristaltic shape transformation in axons exposed to controlled osmotic perturbation. The peristaltic relaxes and the axon recovers its original geometry within minutes. show that instability depends critically on swelling rate volume membrane area regulation are responsible for relaxation. propose occurs via leakage of ions driven by elastic pressure, analyze dynamics taking into account internal structure axon. results obtained provide framework understanding nerve...
We report a systematic investigation of the mechanical properties fibroblast cells using novel cell monolayer rheology (CMR) technique. The new technique provides quantitative rheological parameters averaged over ~106 making experiments highly reproducible. Using this method, we are able to explore broad range responses not accessible other present day techniques. perform harmonic oscillation and step shear or stress reveal different viscoelastic regimes. evolution live under externally...
Cell mechanical properties are fundamental to the organism but remain poorly understood. We report a comprehensive phenomenological framework for complex rheology of single fibroblast cells: superposition elastic stiffening and viscoplastic kinematic hardening. Despite complexity living cell, its can be cast into simple, well-defined rules. Our results reveal key role crosslink slippage in determining cell strength robustness.
Background Despite their notorious diversity, biological cells are mechanically well characterized by only a few robust and universal laws. Intriguingly, the law characterizing nonlinear response to stretch appears self-contradictory. Various cell types have been reported both stiffen soften, or “fluidize” upon stretch. Within classical paradigm of as viscoelastic bodies, this constitutes paradox. Principal Findings Our measurements reveal that minimalistic reconstituted cytoskeletal...
We propose a physical model for the nonlinear inelastic mechanics of sticky biopolymer networks with potential applications to cell mechanics. It consists in minimal extension glassy wormlike chain (GWLC) model, which has recently been highly successful as quantitative mathematical description viscoelastic properties and cells. To extend its scope nonequilibrium situations, where thermodynamic state variables may evolve dynamically, GWLC is furnished an explicit representation kinetics...
Objective.In this paper, we present a detailedin vivocharacterization of the optical and hemodynamic properties human sternocleidomastoid muscle (SCM), obtained through ultrasound-guided near-infrared time-domain diffuse correlation spectroscopies.Approach.A total sixty-five subjects (forty-nine females, sixteen males) among healthy volunteers thyroid nodule patients have been recruited for study. Their SCM (oxy-, deoxy- hemoglobin concentrations, blood flow, oxygen saturation metabolic rate...
Cell-driven plastic remodeling of the extracellular matrix (ECM) is a key regulator driving cell invasion and organoid morphogenesis in 3D. While, mostly, linear properties are reported, nonlinear property used required for these processes to occur. Here, we report on mechanical networks derived from collagen I, Matrigel, related hybrid gels link their response underlying structure. We reveal predominantly behavior Matrigel over wide range strains contrast this highly upon load. show that...
We propose a nonlinear extension of the standard tube model for semidilute solutions freely-sliding semiflexible polymers. Non-affine filament deformations at entanglement scale, renormalisation direct interactions by thermal fluctuations, and geometry large are systematically taken into account. The stiffening response predicted athermal stiff rods1 is found to be thermally suppressed. Instead, we obtain broad linear regime, supporting interpretation shear finite frequencies in polymerised...
Orientation of the division axis can determine cell fate in presence morphogenetic gradients. Understanding how mitotic cells integrate directional cues is therefore an important question embryogenesis. Here, we investigate effect dynamic shear forces on confined cells. We found that human epithelial (hTERT-RPE1) as well MC3T3 osteoblasts align their spindle perpendicular to external force. Spindle orientation appears be a consequence elongation along zero-force direction response shear....
Although branching morphogenesis is central for organogenesis in diverse organs, the underlying self-organizing principles have yet to be identified. Here, we show that invasive human mammary organoids relies on an intricate tension-driven feedback mechanism, which based nonlinear and plastic mechanical response of surrounding collagen network. Specifically, demonstrate collective motion cells within organoid branches generates tension strong enough induce a reorganization network results...
Abstract The non-invasive monitoring of the hemodynamics and metabolism sternocleidomastoid muscle (SCM) during respiration became a topic increased interest partially due to use mechanical ventilation COVID-19 pandemic. Near-infrared diffuse optical spectroscopies were proposed as potential practical monitors recruitment SCM respiratory distress. They can provide clinically relevant information on degree patient’s effort that is needed maintain an optimal minute ventilation, with clinical...
Key Clinical Message Inverted connection of the atrial and ventricular leads is an unusual circumstance during implantation a dual‐chamber pacemaker. Yet, PMT may present in absence complex mechanisms. The detection termination algorithms used by device proved to be efficient for adequate diagnosis treatment.
Thyroid vascularization and hemodynamics become altered in thyroid pathologies could thus inform diagnostics, therapy planning, follow-up. However, the current non-invasive monitoring methods available clinics lack necessary sensitivity and/or are impractical for large-scale deployment. As a step towards proposing new modality, we applied first platform, to our knowledge, designed do simultaneous measurements of neck anatomy microvascular metabolism single probe placement, integrating...