A5020879747- DNA Repair Mechanisms
- Genomics and Phylogenetic Studies
- Telomeres, Telomerase, and Senescence
- Genomics and Chromatin Dynamics
- Genetics, Aging, and Longevity in Model Organisms
- Fungal and yeast genetics research
- Bacterial Genetics and Biotechnology
- Microbial Community Ecology and Physiology
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Protist diversity and phylogeny
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- Animal Virus Infections Studies
- Genetic Neurodegenerative Diseases
- Animal Disease Management and Epidemiology
- Enzyme Structure and Function
- Viral gastroenteritis research and epidemiology
- Reproductive System and Pregnancy
- Mitochondrial Function and Pathology
- Quantum Dots Synthesis And Properties
- Photosynthetic Processes and Mechanisms
University of Chinese Academy of Sciences
2013-2024
South China Agricultural University
2012-2024
State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources
2024
Key Laboratory of Guangdong Province
2024
Wen's Food Group (China)
2024
Shanghai Jiao Tong University
2020-2022
Shanghai Ninth People's Hospital
2020-2022
State Key Laboratory of Oncogene and Related Genes
2020-2021
Center for Excellence in Molecular Cell Science
2015-2019
Chinese Academy of Sciences
2003-2018
Abstract NDH-1 is a key component of the cyclic-electron-transfer around photosystem I (PSI CET) pathway, an important antioxidant mechanism for efficient photosynthesis. Here, we report 3.2-Å-resolution cryo-EM structure ferredoxin (Fd)-NDH-1L complex from cyanobacterium Thermosynechococcus elongatus . The reveals three β-carotene and fifteen lipid molecules in membrane arm NDH-1L. Regulatory oxygenic photosynthesis-specific (OPS) subunits NdhV, NdhS NdhO are close to Fd-binding site whilst...
Abstract Telomerase, a multi-subunit ribonucleoprotein complex, is unique reverse transcriptase that catalyzes the processive addition of repeat sequence to extend telomere end using short fragment its own RNA component as template. Despite recent structural characterizations human and Tetrahymena telomerase, it still mystery how telomerase repeatedly uses template synthesize telomeric DNA. Here, we report cryo-EM structure holoenzyme bound with DNA at resolutions 3.5 Å 3.9 for catalytic...
ABSTRACT Haloarcula hispanica is an extremely halophilic archaeon that has unusually low restriction barrier and therefore significant for studying archaeal genetics, metabolism, virus-host interactions. Here we report the complete genome sequence (3,890,005 bp) of H. strain CGMCC 1.2049, consisting two chromosomes one megaplasmid.
Rad6 and Bre1, ubiquitin-conjugating E2 E3 enzymes respectively, are responsible for histone H2B lysine 123 mono-ubiquitination (H2Bub1) in Saccharomyces cerevisiae. Previous studies have shown that Bre1 regulate telomere length recombination. However, the underlying molecular mechanism remains largely unknown. Here we report H2BK123 mutation results shortening, while inactivation of Ubp8 and/or Ubp10, deubiquitinases H2Bub1, leads to lengthening Rad6-Bre1-dependent manner. In...
Abstract Background While multiple replication origins have been observed in archaea, considerably less is known about their evolutionary processes. Here, we performed a comparative analysis of the predicted (proved part) orc/cdc6 -associated 15 completely sequenced haloarchaeal genomes to investigate diversity and evolution halophilic Archaea. Results Multiple were all analyzed following identification putative ORBs (origin recognition boxes) that are associated with genes. Five these...
Abstract The use of multiple replication origins in archaea is not well understood. In particular, little known about their specific control mechanisms. Here, we investigated the active three replicons a halophilic archaeon, Haloarcula hispanica, by extensive gene deletion, DNA mutation and genome-wide marker frequency analyses. We revealed that individual are specifically dependent on co-located cdc6 genes, single origin/cdc6 pairing essential sufficient for each replicon. Notably,...
KEOPS complex is one of the most conserved protein complexes in eukaryotes. It plays important roles both telomere uncapping and tRNA N6-threonylcarbamoyladenosine (t6A) modification budding yeast. But whether any DNA repair remains unknown. Here, we show that positive damage response homologous recombination-mediated independently its t6A synthesis function. Additionally, displays binding activity vitro, recruited to chromatin at breaks vivo, suggesting a direct role DSB repair....
Abstract The use of multiple origins for chromosome replication has been demonstrated in archaea. Similar to the dormant eukaryotes, some potential archaea appear be inactive during genome replication. We have comprehensively explored origin utilization Haloferax mediterranei . Here we report three active chromosomal by genome-wide profiling, and demonstrate that when these are deleted, a becomes activated. Notably, this cannot further deleted other already absent vice versa Interestingly,...
The presence of minichromosomes is very common in haloarchaea, but little known about the coordination replication between major and minor chromosomes. In this study, we analyzed pHM300, a 321,908-bp minichromosome, which encodes versatile metabolism pathways Haloferax mediterranei. origin pHM300 was predicted 699-bp intergenic region cdc6K tbp4 gene, minimal replicon, consisting an AT-rich flanked by putative recognition boxes (ORBs) adjacent determined assaying for its ability to replicate...
The transporter associated with antigen processing (TAP) translocates antigenic peptides from the cytosol into lumen of endoplasmic reticular and plays a critical role in major histocompatibility complex (MHC) class I molecule-mediated presentation pathway.In this study, porcine TAP1 gene was mapped to pig chromosome 7 (SSC7) closely linked marker SSC2B02 (retention fraction=43%, LOD=15.18).Subcellular localization by transient transfection PK15 cells indicated that protein might be located...
Many haloarchaea are known as polyhydroxyalkanoates (PHAs) producers, but a global and integrated view of the PHA biosynthesis is still lacking in this group archaea. In study, combined proteomic transcriptomic approach was employed Haloarcula hispanica, model haloarchaeon that accumulates poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) under nutrient-limiting conditions with excess carbon source. First, comprehensive proteome reference map established for H. hispanica. A total 936 spots...
Chronological aging of the yeast Saccharomyces cerevisiae is attributed to multi-faceted traits especially those involving genome instability, and has been considered be an model for post-mitotic cells in higher organisms. Telomeres are physical ends eukaryotic chromosomes, essential integrity stability. It remains elusive whether dysregulated telomerase activity affects chronological aging. We employed CDC13-EST2 fusion gene, which tethers telomeres, examine effect constitutively active on...
The conserved shelterin complex caps chromosome ends to protect telomeres and regulate telomere replication. In fission yeast Schizosaccharomyces pombe , consists of telomeric single- double-stranded DNA-binding modules Pot1-Tpz1 Taz1-Rap1 connected by Poz1, a specific component Ccq1. While individual structures the two OB folds Pot1 (Pot1 OB1 -GGTTAC OB2 -GGTTACGGT) are available, structural insight into recognition repeats with spacers complete domain DBD ) remains an open question....
The trimethylation of histone H3 lysine 27 (H3K27me3) is one the most important chromatin modifications, which generally presented as a repressive mark in various biological processes. However, dynamic and global-scale distribution H3K27me3 during porcine embryonic muscle development remains unclear. Here, our study provided comprehensive genome-wide view analyzed matching transcriptome skeletal muscles on days 33, 65, 90 post-coitus from Duroc fetuses. Transcriptome analysis identified...
Extracytoplasmic function (ECF) σ factors, the largest group of alternative play important roles in response to environmental stresses. Tt-RpoE1 is annotated as an ECF factor Thermoanaerobacter tengcongensis. In this study, we revealed that Tt-tolB gene located downstream Tt-rpoE1 encoded cognate anti-σ factor, which could inhibit transcription activity by direct interaction with via its N-terminal domain. By vitro assay, auto-regulation ability was determined, and band shift assay showed...
Haloarchaeal genomes are generally composed of multiple replicons, and each replicon has a single or replication origin(s). The comparative genomic analysis origins from closely related species can be used to reveal the evolutionary mechanisms that account for development origin systems. Multiple have been in silico experimentally investigated Haloarcula hispanica, which raise possibility comparisons species. Thus, we performed comparison H. hispanica with those five additional We...