A5060473886- Reproductive System and Pregnancy
- Genetic and phenotypic traits in livestock
- Cancer-related molecular mechanisms research
- Extracellular vesicles in disease
- Genetic Mapping and Diversity in Plants and Animals
- Reproductive Physiology in Livestock
- Circular RNAs in diseases
- Reproductive Biology and Fertility
- MicroRNA in disease regulation
- Animal Genetics and Reproduction
- Pregnancy and preeclampsia studies
- Animal Virus Infections Studies
- Genetics and Plant Breeding
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- COVID-19 Impact on Reproduction
- T-cell and Retrovirus Studies
- Birth, Development, and Health
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Renal and related cancers
- RNA Research and Splicing
- Metabolomics and Mass Spectrometry Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Pluripotent Stem Cells Research
South China Agricultural University
2018-2025
China Agricultural University
2013-2025
Key Laboratory of Guangdong Province
2020-2024
Ministry of Agriculture and Rural Affairs
2023-2024
Animal Science Research Institute
2022
State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources
2022
Huazhong Agricultural University
2013-2020
Beltsville Agricultural Research Center
2016-2017
Agricultural Research Service
2016
Intramuscular fat (IMF) is an important meat trait relevant to the processing industry and consumer acceptance. To gain a better understanding of genetic determinants IMF, we used composite strategy, including single-locus multi-locus association analyses perform genome-wide studies (GWAS) for IMF in 1,490 Duroc boars. We estimated genomic heritability be 0.23 ± 0.04. A total 30 SNPs were detected as significantly associated with IMF. Of these, five, nine, eight, 21 significant identified by...
Many circular RNAs (circRNAs) have been discovered in various tissues and cell types pig. However, the temporal expression pattern of circRNAs during porcine embryonic muscle development remains unclear. Here, we present a panorama view circRNA at 33-, 65-, 90-days post-coitus (dpc) from Duroc pigs. An unbiased analysis reveals that more than 5,000 specifically express development. The amount complexity is most pronounced skeletal day 33 gestation. Our annotation analyses show "hot-spot"...
Growth traits are important economic of pigs that controlled by several major genes and multiple minor genes. To better understand the genetic architecture growth traits, we performed a weighted single-step genome-wide association study (wssGWAS) to identify genomic regions candidate associated with days 100 kg (AGE), average daily gain (ADG), backfat thickness (BF) lean meat percentage (LMP) in Duroc pig population. In this study, 3945 individuals phenotypic genealogical information, which...
Long non-coding RNAs (lncRNAs) act as competing endogenous and play significant roles in porcine embryo development. Extracellular vesicles (EVs) the uterine fluid (UF) can target deliver maternal endometrial signalling molecules to embryonic trophoblast cells, exerting crucial regulatory effects during implantation. However, specific of lncRNAs carried by UF-EVs implantation period have not been thoroughly reported literature. In present study, high-throughput sequencing biological tools...
ABSTRACT Grifola frondosa antioxidant peptides (GFAP) were prepared through trypsin enzymolysis and characterized. This study conducted a comprehensive assessment of clinical symptoms, colon pathological injuries, levels inflammatory factors, expression inflammation‐related proteins, alterations in gut microbiota composition mice with ulcerative colitis (UC). The findings demonstrated that GFAP effectively mitigated UC, alleviated mucosal damage, reduced infiltration. Specifically,...
The composition of muscle fibers, characterized by distinct contractile and metabolic properties, significantly influences meat quality glucose homeostasis. However, the mechanisms which three-dimensional (3D) genome topology integrates with epigenetic states to regulate fiber specification transformation remain poorly understood. Here, we present an integrative analysis transcriptome, epigenome, 3D architecture in slow-twitch glycolytic extensor digitorum longus (EDL) fast-twitch oxidative...
Extracellular vesicles (EVs) regulate multiple physiological processes. Seminal plasma contains numerous EVs which may deliver functional molecules such as small RNAs (sRNAs) to the sperm. However, RNA profiles in boar seminal extracellular (SP-EVs) and its function has not been characterized. The aim of this study was establish an effective method for isolation SP-EVs, characterize functions sRNAs SP-EVs using deep sequencing technology. Briefly, were isolated by differential...
Abstract Background Carcass traits are important in pig breeding programs for improving pork production. Understanding the genetic variants underlies complex phenotypes can help explain trait variation pigs. In this study, we integrated a weighted single-step genome-wide association study (wssGWAS) and copy number (CNV) analyses to map variations genes associated with loin muscle area (LMA), depth (LMD) lean meat percentage (LMP) Duroc Results Firstly, performed analysis CNV detection using...
N6-methyladenosine (m6A) is the most common modification in eukaryotic RNAs. Accumulating evidence shows m6A methylation plays vital roles various biological processes, including muscle and fat differentiation. However, there a lack of research on lncRNAs' regulating pig muscle-fiber-type conversion. In this study, we identified novel differentially expressed lncRNAs oxidative glycolytic skeletal muscles through RNA-seq, further reported m6A-methylation patterns via MeRIP-seq. We found that...
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection of pregnant pigs can result in congenital ultimately fetal death. Little is known about immune responses to at the maternal-fetal interface fetus itself, or molecular events behind virus transmission disease progression fetus. To investigate these processes, RNA-sequencing two sites, uterine endothelium with adherent placental tissue thymus, was performed 21 days post-challenge on four groups fetuses selected from a large...
Background: In western swine breeds, up to 30% of embryonic losses occur during early pregnancy, and the majority happens implantation. this period, maternal recognition pregnancy begins blastocysts undergo dramatic morphologic changes. As with other species, changes in uterine environment plays an important role process embryo implantation pigs. Erhualian (ER) pigs, one Chinese Taihu are known have highest litter size world. Experiments demonstrated that greater survival on gestation day...
Duroc pigs are famous for their high growth rate, feed conversion efficiency, and lean meat percentage. Given that they have been subjected to artificial selection breeding in multiple countries, various lines with obvious differences production performance formed. In this study, we genotyped 3,770 American (AD) 2,098 Canadian (CD) using the GeneSeek Porcine SNP50 Beadchip dissect genetic potential genes of traits these two pig lines. Population structure detection showed there were...
Heterozygosity can effectively reflect the diverse models of population structure and demographic history. However, genomic distribution heterozygotes correlation between regions heterozygosity (runs heterozygosity, ROHet) phenotypes are largely understudied in livestock. The objective this study was to identify ROHet Duroc pig genome, investigate relationships eight important economic traits. Here, we genotyped 3,770 American (S21) 2,096 Canadian (S22) pigs using 50 K single nucleotide...
Runs of homozygosity (ROH) are widely used to investigate genetic diversity, demographic history, and positive selection signatures livestock. Commercial breeds provide excellent materials reveal the landscape ROH shaped during intense process. Here, we GeneSeek Porcine 50K single-nucleotide polymorphism (SNP) Chip data 3,770 American Duroc (AD) 2,096 Canadian (CD) pigs analyze genome-wide ROH. First, showed that AD had a moderate differentiation with CD pigs, more abundant diversity...
It is one of the important events that trophoblast cells within placental folds differentiate into two types differ in cell shape during development pigs. This study showed all were similar between Yorkshire and Chinese Meishan pigs on day 26 gestation; thereafter, located at top became high columnar, while those base cuboidal 50 gestation. Additionally, 95 gestation, cuboidal, but still remained columnar. The membranous E-cadherin β-catenin strongly co-expressed by columnar very weakly...
Early pregnancy diagnosis in sows can significantly improve the efficiency of pig industry. Exosomes are membrane-covered nanovesicles that transport microRNAs (miRNAs) and other molecular signals between cells. In species, serum exosome-derived miRNAs serve as good biomarkers diseases different physiological states, including status. We hypothesized circulating might be used to differentiate status early several days after insemination pigs. To test this hypothesis, we randomly assigned...
Most pregnancy losses worldwide are caused by implantation failure for which there is a lack of effective therapeutics. Extracellular vesicles considered potential endogenous nanomedicines because their unique biological functions. However, the limited supply ULF-EVs prevents development and application in infertility diseases such as failure. In this study, pigs were used human biomedical model, isolated from uterine luminal. We comprehensively characterized proteins enriched revealed...
The present study aimed to investigate the colonization process of epithelial bacteria attached rumen and intestinal tract tissue during development goats after birth. However, this from fetus adulthood was very limited. In goats, cecum are two important fermentation organs, it is acquisition neonatal microbiome, as well difference between these organs. To characterize microbial establishment dynamic changes in adulthood, we performed 16S rRNA gene sequencing for 106 samples 47 individuals...