A5009689461- Reproductive System and Pregnancy
- Extracellular vesicles in disease
- Genetic and phenotypic traits in livestock
- Genetic Mapping and Diversity in Plants and Animals
- Cancer-related molecular mechanisms research
- Flavonoids in Medical Research
- Reproductive Physiology in Livestock
- Reproductive Biology and Fertility
- Bone and Dental Protein Studies
- MicroRNA in disease regulation
- dental development and anomalies
- RNA modifications and cancer
- Animal Genetics and Reproduction
- Genetics and Plant Breeding
- Natural product bioactivities and synthesis
- Epigenetics and DNA Methylation
- Circular RNAs in diseases
- Wheat and Barley Genetics and Pathology
- Oral microbiology and periodontitis research
- Neurological Disease Mechanisms and Treatments
- Acupuncture Treatment Research Studies
- Traditional Chinese Medicine Analysis
- CRISPR and Genetic Engineering
- Pregnancy and preeclampsia studies
- COVID-19 Impact on Reproduction
South China Agricultural University
2015-2025
Guangdong Medical College
2025
Harbin Medical University
2025
Second Affiliated Hospital of Harbin Medical University
2025
Key Laboratory of Guangdong Province
2020-2024
Ministry of Agriculture and Rural Affairs
2024
Fudan University Shanghai Cancer Center
2021-2024
Poultry Research Institute
2024
Hunan Agricultural University
2021-2024
Shanghai Medical College of Fudan University
2024
Dentin is the major mineralized extracellular matrix of tooth. The organic components dentin consist type I collagen (90%) with 10% noncollagenous proteins, which are also bone. Two sialoprotein and phosphoprotein, have been shown to be tooth-specific being expressed mostly by odontoblast cells. In this study, we screened a mouse molar tooth library for phosphoprotein cDNA clones. Analysis clones resulted in characterization 4420-nucleotide that contained 940-amino acid open reading frame....
To determine potential targets of the S locus receptor kinase (SRK) during Brassica self-incompatibility response, a yeast two-hybrid library was screened with SRK-910 protein domain. Two thioredoxin-h-like clones, THL-1 and THL-2, were found to interact specifically domain not domains from Arabidopsis receptor-like kinases (RLK) RLK4 RLK5. The interaction between confirmed using coimmunoprecipitation experiments fusion proteins produced in Escherichia coli. has thioredoxin activity based on...
Dentin sialophosphoprotein (DSPP) consists of dentin sialoprotein (DSP) and phosphoprotein (DPP). The spatial-temporal expression DSPP is largely restricted during differentiational stages dental cells. plays a vital role in tooth development. It known that an osteoblast-specific transcription factor, Runx2, essential for osteoblast differentiation. However, effects Runx2 on remain unknown. Here, we studied different roles controlling mouse preodontoblast (MD10-F2) odontoblast (MO6-G3) Two...
The trihelix gene family is a plant-specific transcription factor that plays important roles in plant growth, development, and responses to abiotic stresses. However, date, no systemic characterization of the genes has yet been conducted wheat its close relatives. We identified total 94 wheat, as well 22 Triticum urartu, 29 Aegilops tauschii, 31 Brachypodium distachyon. analyzed chromosomal locations orthology relations genes, was found be located on chromosome 7A, 7B, or 7D thereby...
WD40 domains are abundant in eukaryotes, and they essential subunits of large multiprotein complexes, which serve as scaffolds. proteins participate various cellular processes, such histone modification, transcription regulation, signal transduction. regarded crucial regulators plant development processes. However, the systematic identification analysis have yet to be reported wheat. In this study, a total 743 were identified wheat, grouped into 5 clusters 11 subfamilies. Their gene...
Many circular RNAs (circRNAs) have been discovered in various tissues and cell types pig. However, the temporal expression pattern of circRNAs during porcine embryonic muscle development remains unclear. Here, we present a panorama view circRNA at 33-, 65-, 90-days post-coitus (dpc) from Duroc pigs. An unbiased analysis reveals that more than 5,000 specifically express development. The amount complexity is most pronounced skeletal day 33 gestation. Our annotation analyses show "hot-spot"...
Long non-coding RNAs (lncRNAs) act as competing endogenous and play significant roles in porcine embryo development. Extracellular vesicles (EVs) the uterine fluid (UF) can target deliver maternal endometrial signalling molecules to embryonic trophoblast cells, exerting crucial regulatory effects during implantation. However, specific of lncRNAs carried by UF-EVs implantation period have not been thoroughly reported literature. In present study, high-throughput sequencing biological tools...
Abstract Background Immunotherapy is an effective treatment for BRAF V600E-mutant colorectal cancer, but currently, only a few benefit from it. Therefore, exploring new immunotherapy strategies essential. Methods We obtained RNA sequencing data and clinical information of cancer patients the TCGA GEO databases. The impact V600E mutation on tumor microenvironment characteristics, gene expression, signaling pathways was evaluated using bioinformatics approaches. Weighted co-expression network...
The composition of muscle fibers, characterized by distinct contractile and metabolic properties, significantly influences meat quality glucose homeostasis. However, the mechanisms which three-dimensional (3D) genome topology integrates with epigenetic states to regulate fiber specification transformation remain poorly understood. Here, we present an integrative analysis transcriptome, epigenome, 3D architecture in slow-twitch glycolytic extensor digitorum longus (EDL) fast-twitch oxidative...
Although tsRNA has been demonstrated to modulate various physiological processes analogous miRNA, the potential regulatory functions and mechanisms of tsRNAs related pharmacological effects small molecule drugs remain unclear. Herein, it is shown that eurycomalactone (ELT), a natural product, can reversibly switch hepatocellular carcinoma (HCC) PLC/PRF/5 HUH7 cells into quiescent state. This quiescence characterized by cell proliferation inhibition without cytotoxicity, cycle arrest at G0/G1...
Extracellular vesicles (EVs) regulate multiple physiological processes. Seminal plasma contains numerous EVs which may deliver functional molecules such as small RNAs (sRNAs) to the sperm. However, RNA profiles in boar seminal extracellular (SP-EVs) and its function has not been characterized. The aim of this study was establish an effective method for isolation SP-EVs, characterize functions sRNAs SP-EVs using deep sequencing technology. Briefly, were isolated by differential...
Background/Aims: Skeletal muscle plays an essential role in the body movement. However, injuries to skeletal are common. Lifelong maintenance of function largely depends on preserving regenerative capacity muscle. Muscle satellite cells proliferation, differentiation, and myoblast fusion play important regeneration after injury. Therefore, understanding mechanisms associated with development during is for devising alternative treatments injury future. Methods: Edu staining, qRT-PCR western...
Litter size in pigs is a major factor affecting the profitability pig industry. The peri-implantation window characterized by coordinated interactions between maternal uterine endometrium and rapidly elongating conceptuses represents period of time during which large percentage developing are lost. However, gene expression regulatory networks contributing to establishment maternal: placental interface remain poorly understood. We endometrial profile stage development comparing two breeds...
Plasma carcinoembryonic antigen (CEA), carbohydrate 19-9 (CA19-9), and cancer 72-4 (CA72-4) are common markers which useful in the diagnosis prognosis of GC. However, their sensitivity specificity GC remain unsatisfactory. Identification diagnosed-biomarkers would be great value.Evaluate diagnostic prognostic value LINC00086 miR-214 GC.In this study, we determined expression by qRT-PCR. Additionally, investigated relationship between various clinicopathological features patients or...
The Duroc × (Landrace Yorkshire) hybrid pigs (DLY) are the most popular commercial pigs, providing consumers with largest source of pork. In order to gain more insights into genetic architecture economically important traits in we performed a genome-wide association study (GWAS) using GeneSeek Porcine 50 K SNP Chip map markers and genes associated body conformation (BCT) 311 DLY pigs. quantitative analyzed included weight (BW), carcass length (CL), (BL), height (BH), mass index (BMI). BMI...
N6-methyladenosine (m6A) is the most common modification in eukaryotic RNAs. Accumulating evidence shows m6A methylation plays vital roles various biological processes, including muscle and fat differentiation. However, there a lack of research on lncRNAs' regulating pig muscle-fiber-type conversion. In this study, we identified novel differentially expressed lncRNAs oxidative glycolytic skeletal muscles through RNA-seq, further reported m6A-methylation patterns via MeRIP-seq. We found that...