A5013509841- Mesenchymal stem cell research
- Wound Healing and Treatments
- Circular RNAs in diseases
- Animal Virus Infections Studies
- Reproductive Biology and Fertility
- Muscle Physiology and Disorders
- Prenatal Screening and Diagnostics
- Animal Genetics and Reproduction
- Renal and related cancers
- MicroRNA in disease regulation
- Diabetic Foot Ulcer Assessment and Management
- Viral Infections and Immunology Research
- Tissue Engineering and Regenerative Medicine
- Erythrocyte Function and Pathophysiology
- Viral gastroenteritis research and epidemiology
- Periodontal Regeneration and Treatments
- RNA Interference and Gene Delivery
- Reconstructive Surgery and Microvascular Techniques
- Genetic Mapping and Diversity in Plants and Animals
- Bone Tissue Engineering Materials
- RNA and protein synthesis mechanisms
- Osteoarthritis Treatment and Mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Metalloenzymes and iron-sulfur proteins
- Spaceflight effects on biology
University of Chinese Academy of Sciences
2023-2025
Wenzhou Medical University
2023-2025
First Affiliated Hospital of Wenzhou Medical University
2023-2025
Sun Yat-sen University
2020-2023
Chinese Academy of Sciences
2022
South China Agricultural University
2014-2021
Breast reconstruction after mastectomy aims to restore breast appearance and function. Current scaffolds often fail meet the complex clinical demands of post-mastectomy reconstruction, which include personalized shaping, prevention tumor recurrence metastasis, promotion adipose tissue regeneration. This study address these challenges by combining a readily processable biopolymer, polycaprolactone (PCL), with multifunctional bioactive ceramic, cobalt orthosilicate (Co2SiO4, CoSi) form...
Background/Aims: Skeletal muscle plays an essential role in the body movement. However, injuries to skeletal are common. Lifelong maintenance of function largely depends on preserving regenerative capacity muscle. Muscle satellite cells proliferation, differentiation, and myoblast fusion play important regeneration after injury. Therefore, understanding mechanisms associated with development during is for devising alternative treatments injury future. Methods: Edu staining, qRT-PCR western...
Abstract Background Diabetic cutaneous ulcers (DCU) are a complication of diabetes with diabetic foot being the most common, and wounds difficult to heal, increasing risk bacterial infection. Cell-based therapy utilizing mesenchymal stem cells (MSCs) is currently investigated as therapeutic avenue for both chronic severe burns. Wharton’s jelly cell (WJMSC) PF-127 hydrogel sodium ascorbyl phosphate (SAP) improved skin wound healing in mice. Whether this combination strategy helpful remains be...
Abstract Background Factors such as poor engraftment, retention, and survival of the transplanted stem cells are deemed to limit their therapeutic efficacy for wound regeneration. Hence, it is necessary explore these issues in order resolve them. In this study, we aim investigate role Pluronic F-127 (PF-127) hydrogel plus antioxidant sodium ascorbyl phosphate (SAP) enhancing Wharton’s jelly mesenchymal cell (WJMSC)-mediated effectiveness on full-thickness skin healing mice. Methods First,...
Muscle development requires myoblast differentiation and muscle fiber formation. Myod family inhibitor (Mdfi) inhibits myogenic regulatory factors in NIH3T3 cells, but how Mdfi regulates is still unclear. In the present study, we constructed an Mdfi-overexpression (Mdfi-OE) C2C12 cell line by CRISPR/Cas9 system performed RNA-seq on Mdfi-OE wild-type (WT) cells. The results showed that calcium signaling pathway was most significant. We also established networks of type transformation...
Since pork accounts for about 40% of global meat consumption, the pig is an important economic animal production. Pig also a useful medical model humans due to its similarity in size and physiology. Understanding mechanism muscle development has great implication breeding human health. Previous studies showed porcine satellite cells (PSCs) are postnatal skeletal growth, Notch1 signaling pathway miRNAs regulate development. signal regulates transcription certain types which further affects...
Notch signaling as a conserved cell fate regulator is involved in the regulation of quiescence, proliferation, differentiation and postnatal tissue regeneration. However, how regulates porcine satellite cells (PSCs) has not been elucidated. We stably transfected Notch1 intracellular domain (N1ICD) into PSCs to analyze gene expression profile miRNA-seq. The analysis identified 295 differentially-expressed genes (DEGs) proliferating-N1ICD (P-N1ICD) nine DEGs on differentiating-N1ICD (D-N1ICD),...
Hydroactive ® Gel promotes WJMSCs-mediated diabetic wound antifibrotic healing through promoting macrophage transformation, facilitating cell proliferation and angiogenesis, prolonging retention time.
Mdfi, an inhibitor of myogenic regulatory factors, is involved in myoblast development and muscle fiber type transformation. However, the network Mdfi regulating myoblasts has not been revealed. In this study, we performed microRNAs (miRNAs)-seq on overexpression (Mdfi-OE) wild-type (WT) C2C12 cells to establish networks. Comparative analyses Mdfi-OE vs. WT identified 66 differentially expressed miRNAs (DEMs). Enrichment analysis target genes suggested that DEMs may be differentiation...
Objective: At present, there is an urgent need to develop a novel and practical therapeutic approach accelerate the healing of acute wounds. Mesenchymal stem cell (MSC)-based therapy emerging as promising for skin However, are still challenges in clinical application this strategy, such low survivability, retention time, less engraftment Approach: Wharton's jelly mesenchymal cells (WJMSCs) were seeded into three-dimensional (3D) gelatin microspheres (GMs) identify biocompatibility GMs....
Abstract Background High-temperature requirement protease A2 (HtrA2/Omi) is a mitochondrial chaperone that highly conserved from bacteria to humans. It plays an important role in homeostasis and apoptosis. In this study, we investigated the of HtrA2 mouse oocyte maturation. Methods The maturation was by employing knockdown (KD) or overexpression (OE) young old germinal vesicle (GV) oocytes. We employed immunoblotting, immunostaining, fluorescent intensity quantification test on GV...
Abstract Premature ovarian failure (POF) is a complex disease affecting an increasing number of young women. Traditional treatments are limited in low efficacy and side effects. Wharton's jelly mesenchymal stem cells (WJMSCs) therapy holds promise the treatment POF. However, clinical application WJMSCs hindered by challenges such as cell viability, significant loss, poor survival rates. In this study, we prepared three‐dimensional chitosan microspheres (CSM). CSM loading substantial improved...
Abstract Background High-temperature requirement protease A2 (HtrA2/Omi) is a mitochondrial chaperone highly conserved from bacteria to humans. It plays an important role in homeostasis and apoptosis. In this study, we investigated the of HtrA2 mouse oocyte maturation. Methods The maturation was by employing knockdown (KD) or overexpression (OE) young old GV oocytes. We employed immunoblotting, immunostaining, fluorescent intensity quantification test on progression, spindle assembly...
Muscle necrosis and angiogenesis are two major difficulties faced in the treatment of lower limb ischemic diseases. In this study, a triple-functional Sr/Si-containing bioceramic/alginate composite hydrogel with bioactivity simultaneously enhancing angiogenesis, regulating inflammation inhibiting muscle was designed to treat This sustainedly releases bioactive Sr2+ SiO32- ions synergistically stimulate create new collateral capillaries re-build blood supply. addition, directly promotes...