Circular RNAs (circRNAs) are a special kind of non-coding that implicated in cancer malignant behavior, including glycolysis. However, their contributions to gastric (GC) cell glycolysis still poorly understood. In the present study, we aimed investigate glycolysis-related role circ-MAT2B GC.Gene expression was determined by qRT-PCR analysis. Protein level detected Western blot. The CCK-8, colony and EdU assays were carried out assess GC viability, formation DNA synthesis rate. Glycolysis...

Abstract STUDY QUESTION Do inactivated coronavirus disease-2019 (COVID-19) vaccines affect IVF outcomes among the vaccine recipients? SUMMARY ANSWER The receipt of COVID-19 before ovarian stimulation has little effect on IVF, including outcomes, embryo development and pregnancy rates. WHAT IS KNOWN ALREADY Limited studies have reported that do not function, or outcomes. DESIGN, SIZE, DURATION This was a retrospective cohort study performed at Third Affiliated Hospital Guangzhou Medical...

Does inoculation with inactivated vaccines against coronavirus disease 2019 (Covid-19) before frozen-thawed embryo transfer (FET) affect live birth and neonatal outcomes?Inactivated Covid-19 did not undermine outcomes of women planning for FET.Accumulating reports are now available indicating the safe use mRNA in pregnant lactating women, a few indicate that they associated adverse effects on ovarian stimulation or early pregnancy following IVF. Evidence about safety is very limited.This...

Β-thalassemia is one of the global health burdens. The CD41-42 (-TCTT) mutation at HBB most prevalent pathogenic β-thalassemia in both China and Southeast Asia. Previous studies focused on repairing patient-specific induced pluripotent stem cells, which were subsequently differentiated into hematopoietic progenitor cells (HSPCs) for transplantation. In this study, we directly applied CRISPR/Cas9-based gene editing therapy to correct patient-derived HSPCs. effective by Cas9:sgRNA...

ABSTRACT Objective To determine the optimal luteinising hormone (LH) level on trigger day and its impact pregnancy outcomes in gonadotropin‐releasing (GnRH) antagonist protocols using a data‐driven approach. Design Retrospective cohort study. Setting Third Affiliated Hospital of Guangzhou Medical University. Population Overall, 6107 vitro fertilisation/intra‐cytoplasmic sperm injection fresh embryo transfer cycles with GnRH‐antagonist were performed between January 1, 2018 February 2023....

The impact of paternal age on reproduction, especially using assisted reproductive technologies, has not been well studied to date. To investigate the effect outcomes, here we performed a retrospective analysis 2,627 intracytoplasmic sperm injection (ICSI) cycles at Reproductive Medicine Center Third Affiliated Hospital Guangzhou Medical University (China) between January 2007 and May 2015. Effect embryo quality [number fertilized oocytes, 2 pronucleus zygotes (2PNs), viable embryos,...

Controlled ovarian stimulation (COS) has a negative effect on the endometrial receptivity compared with natural menstrual cycle. Whether it's necessary to postpone first frozen embryo transfer (FET) following freeze-all strategy in order avoid any residual consequent COS was inconclusive.The purpose of this retrospective study explore whether delayed FET improve live birth rate and neonatal outcomes stratified by protocols after strategy.A total 4404 patients who underwent cycle were...

Objective: The objective of this retrospective study was to evaluate clinical outcomes early cumulus cells removal and rescue intracytoplasmic sperm injection (ICSI) in high-risk patients fertilization failure during human vitro (IVF).Methods: A total 5,518 were enrolled between January 2014 December 2016. Of which 505 couples performed short insemination with >30% rate included short-term IVF group, 102 detected (TFF) treated ICSI (R-ICSI group), 4911 underwent conventional overnight...

Abstract Background Multiple pregnancies are associated with significant complications and health risks for both mothers infants. Single blastocyst transfer (SBT) is a logical effective measure to reduce the incidence of multiple pregnancy assisted reproductive technology (ART). Whether it suitable everyone undergoing SBT was inconclusive, in view consideration embryo quality patients’ age. Therefore, this study aimed explore live birth rate (LBR) neonatal outcomes different quantities...

The predictive value of anti-Müllerian hormone (AMH) in Chinese women undergoing vitro fertilization (IVF) treatment is data deficient. To determine the attributes AMH IVF, oocyte yield, cycle cancellation, and pregnancy outcomes were analyzed. All patients initiating their first IVF with gonadotropin-releasing agonist our center from October 2013 through December 2014 included, except diagnosed polycystic ovarian syndrome. Serum samples collected prior to used serum levels. A total 4017...

Gestational diabetes mellitus (GDM) is associated with a heightened level of oxidative stress, which characterized by the overproduction reactive oxygen species (ROS) from mitochondria. Previous studies showed that mitochondrial dysfunction regulated dynamin-related protein 1 (Drp1) and p66Shc in GDM.The aim was to investigate expression Drp1 their possible mechanisms pathogenesis GDM.A total 30 pregnant women, 15 GDM without GDM, were enrolled. Peripheral blood mononuclear cells placental...

Polycystic ovary syndrome (PCOS) is an endocrine-related follicular developmental disorder that affects 50 %-70 % of reproductive-aged women diagnosed with ovulation-related infertility. Abnormal proliferation and apoptosis granulosa cells (GCs) are thought to be the critical factors leading abnormal maturation follicles. It has been shown microRNAs (miRNAs) exert a significant influence in pathogenesis PCOS; however, relationship between miRNA, PCOS, GC not entirely understood.To clarify...

Although preimplantation genetic test (PGT) has been used worldwide, few studies investigated the effect of trophectoderm biopsy blastocysts on early embryo development. This study aimed to investigate whether (TE) for a PGT affected serum β-human chorionic gonadotropin (hCG) levels 14 days after transfer.This was retrospective cohort conducted at Third Affiliated Hospital Guangzhou Medical University. The population comprised pregnant women undergoing transfer single vitrified-warmed...

Abstract Background High-quality single blastocyst transfer (SBT) is increasingly recommended to patients because of its acceptable pregnancy outcomes and significantly reduced multiple rate compared double (DBT). However, there no consensus on whether this strategy also suitable for poor-quality blastocysts. Moreover, the effect development speed blastocysts has been controversial. Therefore, study aimed explore effects morphology neonatal during frozen embryo (FET) cycle ultimately provide...

Objective This study aimed to develop multiphase big-data-based prediction models of ovarian hyperstimulation syndrome (OHSS) and a smartphone app for risk calculation patients’ self-monitoring. Methods Multiphase were developed from retrospective cohort database 21,566 women January 2017 December 2020 with controlled stimulation (COS). There 17,445 included in the final data analysis. Women randomly assigned either training (n = 12,211) or validation 5,234). Their baseline clinical...

Abstract Background There is insufficient evidence regarding the impact of dual trigger on oocyte maturity and reproductive outcomes in high responders. Thus, we aimed to explore effect gonadotropin-releasing hormone agonist (GnRHa) alone or combined with different low-dose human chorionic gonadotropin (hCG) regimens rates maturation cumulative live birth responders who underwent a freeze-all strategy GnRH antagonist cycles. Methods A total 1343 cycles were divided into three groups...

Background Vaccine hesitancy was found in couples seeking artificial reproductive technology (ART) services. As the main vaccine used China, investigations into influence of inactivated coronavirus disease 2019 (COVID-19) vaccines on human fertility is needed. Methods This retrospective cohort study included data COVID-19 vaccination, clinical characteristics, and outcome 1,000 intrauterine insemination (IUI) cycles 653 from March 2021 to 2022 a single university hospital-based center for...

Blastocyst developmental speed, morphological grading and patient age are associated with pregnancy outcomes of frozen-thawed cycles. This study aimed to compare the clinical neonatal between poor-quality D5 blastocysts good-quality D6 stratified by age.A total 1,623 cycles were divided into two groups: group A (n = 723) received one blastocyst; B 900) blastocyst. Pregnancy compared among four groups 35 years age.When patients in same group, there was no significant difference terms age,...

Background No studies have been done to examine the efficacy of IVF and intrauterine insemination (IUI) for treatment young patients with unexplained infertility low ovarian reserve, although it is becoming an increasingly significant indication in-vitro fertilization (IVF). The goal this research was compare IUI on Poseidon group 3 (PG3&UI). Methods This a retrospective analysis PG3&UI who had IVF/intracytoplasmic sperm injection (ICSI) or at Third Affiliated Hospital...

Abstract Background There remains a lack of evidence to demonstrate whether the initiation time estrogen stimulation is flexible in proliferative endometrial phase during artificial cycle for frozen-thawed embryo transfer (AC-FET). Methods FET records were retrospectively reviewed from large university-affiliated reproductive medicine center. Only patients who undergoing their first with single blastocyst AC-FET cycles included: thereby 660 recruited, and grouped according day usage as early...

Assisted reproductive technologies (ART) are widely used to treat infertility. Emerging evidence suggested that ART was associated with perinatal or neonatal problems, however, little is known about the related risk factors. Here using 21136 cases, we determined impacts of parental physiological conditions in mediated pregnancy outcomes. In addition, further evaluated effects three different methods (frozen-thawed embryo transfer [FET], vitro fertilization [IVF] and intracytoplasmic sperm...

Objective To investigate the pregnancy outcomes of patients with low serum β-hCG level 14 days after day 3 embryo transfer.Methods A retrospective study was performed 723 a between 5 and 100 mIU/ml transfer. Pregnancy (live birth, clinical pregnancy) were analyzed according to female patients' age. ROC curves plotted indicate threshold for prediction live birth.Results Of <100 mIU/mL, 85.6% (619) had biochemical pregnancy, only 14.4% (104) (including 4.3% 3.7% ectopic 6.1% early...