A5102916235- RNA modifications and cancer
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- Cardiovascular Function and Risk Factors
- Cardiomyopathy and Myosin Studies
- Genomics and Rare Diseases
- Genomic variations and chromosomal abnormalities
- Genetic Mapping and Diversity in Plants and Animals
- Genomics and Phylogenetic Studies
- MicroRNA in disease regulation
- Genetic Associations and Epidemiology
- vaccines and immunoinformatics approaches
- Cardiac Imaging and Diagnostics
- Telomeres, Telomerase, and Senescence
- Fuel Cells and Related Materials
- Virology and Viral Diseases
- Muscle Physiology and Disorders
- Pulmonary Hypertension Research and Treatments
- Microtubule and mitosis dynamics
- Gene expression and cancer classification
- Cancer-related gene regulation
- Cardiovascular Disease and Adiposity
First Affiliated Hospital Zhejiang University
2019-2024
Zunyi Medical University
2024
Simons Foundation
2024
University of California, Los Angeles
2017-2021
Children's Hospital of Philadelphia
2020-2021
Zhejiang University
2020
Shanghai Chest Hospital
2019
South China Agricultural University
2012-2018
University of Hong Kong
2017
Chinese University of Hong Kong
2017
We reported an integrated database of Compendium Protein Lysine Modifications (CPLM; http://cplm.biocuckoo.org) for protein lysine modifications (PLMs), which occur at active ε-amino groups specific residues in proteins and are critical orchestrating various biological processes. The CPLM was updated from our previously developed Acetylation (CPLA), contained 7151 acetylation sites 3311 proteins. Here, we manually collected experimentally identified substrates 12 types PLMs, including...
Steps of mRNA maturation are important gene regulatory events that occur in distinct cellular locations. However, transcriptomic analyses often lose information on the subcellular distribution processed and unprocessed transcripts. We generated extensive RNA-seq data sets to track across locations mouse embryonic stem cells, neuronal progenitor postmitotic neurons. find disparate patterns RNA enrichment between cytoplasmic, nucleoplasmic, chromatin fractions, with some genes maintaining more...
Whole genome sequencing (WGS) is a promising strategy to unravel variants or genes responsible for human diseases and traits. However, there lack of robust platforms comprehensive downstream analysis. In the present study, we first proposed three novel algorithms, sequence gap-filled gene feature annotation, bit-block encoded genotypes sectional fast access text lines address fundamental problems. The algorithms then formed infrastructure parallel computing framework, KGGSeq, integrating...
Abstract Recent studies have indicated that different post-translational modifications (PTMs) synergistically orchestrate specific biological processes by crosstalks. However, the preference of crosstalk among PTMs and evolutionary constraint on PTM need further dissections. In this study, in situ at same positions three tyrosine including sulfation, nitration phosphorylation were systematically analyzed. The experimentally identified sites collected integrated with reliable predictions to...
It remains challenging to predict regulatory variants in particular tissues or cell types due highly context-specific gene regulation. By connecting large-scale epigenomic profiles expression quantitative trait loci (eQTLs) a wide range of human tissues/cell types, we identify critical chromatin features that variant potential. We present cepip, joint likelihood framework, for estimating variant's probability context-dependent manner. Our method exhibits significant GWAS signal enrichment...
A major question in human genetics is how sequence variants of broadly expressed genes produce tissue- and cell type-specific molecular phenotypes. Genetic variation alternative splicing a prevalent source transcriptomic proteomic diversity populations. We investigated quantitative trait loci (sQTLs) 1,209 samples from 13 brain regions, using RNA sequencing (RNA-seq) genotype data the Genotype-Tissue Expression (GTEx) project. Hundreds sQTLs were identified each region. Some shared across...
Background/Aims: Skeletal muscle plays an essential role in the body movement. However, injuries to skeletal are common. Lifelong maintenance of function largely depends on preserving regenerative capacity muscle. Muscle satellite cells proliferation, differentiation, and myoblast fusion play important regeneration after injury. Therefore, understanding mechanisms associated with development during is for devising alternative treatments injury future. Methods: Edu staining, qRT-PCR western...
We reported an updated database of MiCroKiTS 4.0 (http://microkit.biocuckoo.org) for proteins temporally and spatially localized in distinct subcellular positions including midbody, centrosome, kinetochore, telomere mitotic spindle during cell division/mitosis. The was from our previously developed MiCroKit 3.0, which contained 1489 mostly forming super-complexes at centrosome kinetochore seven eukaryotes. Since the apparatus are critical division, two were also integrated. From scientific...
Abstract Idiopathic dilated cardiomyopathy (IDCM), characterized by ventricular dilation and impaired systolic function, is a primary resulting in heart failure. During contraction, the Z ‐line responsible for transmitting force between sarcomeres also hot spot muscle cell signalling. Mutations proteins have been linked to cardiomyopathies both humans mice. Actinin‐associated LIM protein (ALP) enigma homolog (ENH), encoded PDLIM3 PDLIM5 , are components of cytoskeleton localize ‐line. A or...
Abstract Recently, a highly dangerous bird flu has infected over 130 patients in China and the outbreak was attributed to novel avian-origin H7N9 virus. Here, we performed systematic analysis of We clarified controversial viewpoint on neuraminidase (NA) origin confirmed it reassorted from Korean wild birds with higher confidence, whereas common ancestors pathogenic genes existed only one or two years ago. Further NA sequences suggested that most variations are not drug resistant current...
Abstract Aims Circular RNAs (circRNAs) are considered important regulators of biological processes, but their impact on atherosclerosis development, a key factor in coronary artery disease (CAD), has not been fully elucidated. We aimed to investigate potential use patients with CAD and the pathogenesis atherosclerosis. Methods results Patients stable angina (SA) or acute syndrome (ACS) controls were selected for transcriptomic screening quantification circRNAs blood cells. stained carotid...
Abstract Motivation: Exome sequencing studies have facilitated the detection of causal genetic variants in yet-unsolved Mendelian diseases. However, identification disease genes among a list candidates an exome study is still not fully settled, and it often difficult to prioritize candidate for follow-up studies. The inheritance mode provides crucial information understanding diseases, but none existing gene prioritization tools utilize this information. Results: We examined characteristics...
Rationale: Cardiomyocytes (CMs) undergo dramatic structural and functional changes in postnatal maturation; however, the regulatory mechanisms remain greatly unclear.Cypher/Z-band alternatively spliced PDZ-motif protein (ZASP) is an essential sarcomere component maintaining Z-disc stability.Deletion of mouse Cypher mutation human ZASP result dilated cardiomyopathy (DCM).Whether Cypher/ZASP participates CM maturation thereby affects cardiac function has not been answered.Methods:...
Background: Dilated cardiomyopathy (DCM) is a major cause of heart failure worldwide. The Z-line protein Cypher/Z-band alternatively spliced PDZ-motif (ZASP) closely associated with DCM, both clinically and in animal models. Our earlier work revealed Cypher/ZASP as PKA-anchoring (AKAP) that tethers PKA to phosphorylate target substrates. However, the downstream effectors regulated by AKAP their relevance DCM remain largely unknown. Methods Results: For identification candidate substrates,...
<title>Abstract</title> Pseudorabies virus (PRV) is an alpha-herpesvirus capable of infecting pigs, resulting in reproductive disorders sows, orchitis boars, piglet diarrhea, and various other diseases, thus inflicting significant economic losses on the swine industry. The advancement immunoinformatic computer epitope prediction technologies holds promise designing optimal candidate vaccines. Therefore, this study, based non-conserved properties PRV gB proteins adsorption acting viruses, we...
We aimed to study the Meta-analysis Global Group in Chronic Heart Failure (MAGGIC) risk model's prognostic value and relationship with left ventricular remodeling dilated cardiomyopathy.Dilated cardiomyopathy patients were prospectively recruited underwent clinical assessments. MAGGIC score was calculated. Patients followed up for adverse events echocardiography. Primary endpoints all-cause mortality first rehospitalization due heart failure. Secondary endpoint defined as a decline ejection...