A5016547254- Postharvest Quality and Shelf Life Management
- Plant Gene Expression Analysis
- Quasicrystal Structures and Properties
- RNA modifications and cancer
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Genomics and Phylogenetic Studies
- Autophagy in Disease and Therapy
- Advances in Cucurbitaceae Research
- MicroRNA in disease regulation
- Adhesion, Friction, and Surface Interactions
- Cancer, Hypoxia, and Metabolism
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Agricultural pest management studies
- Soybean genetics and cultivation
- Solidification and crystal growth phenomena
- Plant biochemistry and biosynthesis
- Lightning and Electromagnetic Phenomena
- Material Dynamics and Properties
- Biofuel production and bioconversion
- Cancer Cells and Metastasis
- Plant Physiology and Cultivation Studies
Beijing Academy of Agricultural and Forestry Sciences
2020-2024
Ningxia University
2022-2024
Yangzhou University
2024
Nanjing Forestry University
2024
Ministry of Agriculture and Rural Affairs
2021-2024
Qingdao University
2024
Affiliated Hospital of Qingdao University
2024
Nanjing Normal University
2023
Tianjin Agricultural University
2023
China Agricultural University
2021-2022
Abstract Tumor dormancy has important implications for early detection and treatment of cancer. Lack experimental models limited clinical accessibility constitute major obstacles to the molecular characterization dormant tumors. We have developed in which human tumors remain a prolonged period time (>120 days) until they switch rapid growth become strongly angiogenic. These angiogenic retain their ability grow fast once injected new mice. hypothesized that undergo stable genetic...
Tomato is one of the most important vegetable crops in world and a model plant used to study ripening climacteric fleshy fruit. During process tomato fruit, flavor aroma metabolites, color, texture hormones undergo significant changes. However, low temperatures delayed inhibiting compounds ethylene production. Metabolomics transcriptomics analyses fruit stored under temperature (LT, 5 °C) room (RT, 25 were carried out investigate effects storage on physiological changes after harvest. The...
Abstract Cucurbitaceae is one of the most genetically diverse plant families in world. Many them are important vegetables or medicinal plants and widely distributed worldwide. The rapid development sequencing technologies bioinformatic algorithms has enabled generation genome sequences numerous species. This greatly facilitated research on gene identification, evolution, genetic variation, molecular breeding cucurbit crops. So far, 18 different species belonging to tribes Benincaseae,...
Transfer of cellular material via tunneling nanotubes (TNT) was recently discovered as a novel mechanism for intercellular communication. The role exchange in communication renal epithelium is not known. Here we report extensive spontaneous cargo vesicles and organelles between primary human proximal tubular epithelial cells (RPTEC). Cells were labeled with two different quantum dot nanocrystals (Qtracker 605 or 525) quantified by high-throughput fluorescence imaging FACS analysis. In...
Abstract Protein phosphorylation is one of the most important post-translational modifications (PTMs) and regulates a broad spectrum biological processes. Recent progresses in phosphoproteomic identifications have generated flood sites, while integration these sites an urgent need. In this work, we developed curated database dbPAF, containing known H. sapiens , M. musculus R. norvegicus D. melanogaster C. elegans S. pombe cerevisiae . From scientific literature public databases, totally...
Protein methylation is an essential posttranslational modification (PTM) mostly occurs at lysine and arginine residues, regulates a variety of cellular processes. Owing to the rapid progresses in large-scale identification sites, available data set was dramatically expanded, more attention has been paid on specific types residues. Here, we briefly summarized current computational prediction which provided accurate, efficient approach contrast with labor-intensive experiments. We collected...
Abstract Chayote ( Sechium edule ) is an agricultural crop in the Cucurbitaceae family that rich bioactive components. To enhance genetic research on chayote, we used Nanopore third-generation sequencing combined with Hi–C data to assemble a draft chayote genome. A chromosome-level assembly anchored 14 chromosomes (N50 contig and scaffold sizes of 8.40 46.56 Mb, respectively) estimated genome size as 606.42 which large for Cucurbitaceae, 65.94% (401.08 Mb) comprising repetitive sequences;...
Apricot (Prunus armeniaca L.) fruits are highly perishable and prone to quality deterioration during storage transportation. To investigate the effects of LED white light treatment on postharvest ripening using metabolomics, transcriptomics, ATAC-Seq analysis. Fruits were exposed 5 μmol m-2 s-1 for 12 hours followed by darkness at 20 °C daily days. The treatments physiological nutritional evaluated. These data combined with transcriptomic, metabolomic, from taken Day 8 provide insight into...
Abstract “Golden Hook” beans turn purple to different degrees due the compositions and contents of flavonoids during storage. At same time, quality losses softening or pathogen invasion, deteriorations flavor associated with carbohydrate metabolism occur. To reveal effect nitric oxide (NO) generated from sodium nitroprusside (SNP) on postharvest beans, we used metabolome, transcriptome, assay for transposase‐accessible chromatin high‐throughput sequencing approaches compare at time treatment...
Tumor dormancy refers to a critical stage in cancer development which tumor cells remain occult for prolonged period of time until they eventually progress and become clinically apparent. We previously showed that the switch dormant tumors fast-growth is angiogenesis dependent requires stable transcriptional reprogramming cells. Considering microRNAs (miRs) as master regulators transcriptome, we sought investigate their role control dormancy. report here identification consensus set 19 miRs...
Recent studies have demonstrated that dysregulation of macroautophagy/autophagy may play a central role in the pathogenesis neurodegenerative disorders, and induction autophagy protects against toxic insults aggregate-prone proteins by enhancing their clearance. Thus, has become promising therapeutic target diseases. In this study, quantitative phosphoproteomic profiling together with computational analysis was performed to delineate phosphorylation signaling networks regulated 2 natural...
Abstract Snake gourd ( Trichosanthes anguina L.), which belongs to the Cucurbitaceae family, is a popular ornamental and food crop species with medicinal value grown in many parts of world. Although progress has been made its genetic improvement, organization, composition, evolution snake genome remain largely unknown. Here, we report high-quality assembly for gourd, comprising 202 contigs, total size 919.8 Mb an N50 20.1 Mb. These findings indicate that one largest genomes sequenced date....
Transforming growth factor-β1 (TGF-β1) and -β2 are correlated with poorer prognosis in gastric cancer (GC), which act both tumor immune cells. However, their expressions precancer tumor-cell interactions peripheral blood mononuclear cells (PBMCs) remain unclear. Protein levels of TGF-β1 were analyzed by immunohistochemistry corresponding mRNA determined quantitative real-time polymerase chain reaction 93 surgical biopsy specimens. Serum TGF-β concentration was detected enzyme-linked...
Tumor dormancy is a highly prevalent stage in cancer progression. We have previously generated and characterized vivo experimental models of human tumor which micro-tumors remain occult until they spontaneously shift into rapid growth. showed that the dormant undergo stable microRNA (miRNA) switch during their transition from to fast-growing phenotype reported identification consensus signature dormancy-associated miRNAs (DmiRs). miRNA-190 (miR-190) among most upregulated DmiRs all tumors...
Macroautophagy/autophagy is a highly conserved process for degrading cytoplasmic contents, determines cell survival or death, and regulates the cellular homeostasis. Besides ATG proteins, numerous regulators together with various post-translational modifications (PTMs) are also involved in autophagy. In this work, we collected 4,237 experimentally identified proteins regulated autophagy death pathways from literature. Then computationally potential orthologs of known developed comprehensive...
Several physiological changes occur during fruit storage, which include the regulation of genes, metabolisms and transcription factors. In this study, we compared 'JF308' (a normal tomato cultivar) 'YS006' storable to determine difference in accumulated metabolites, gene expression, accessible chromatin regions through metabolome, transcriptome, ATAC-seq analysis. A total 1006 metabolites were identified two cultivars. During storage time, sugars, alcohols flavonoids found be more abundant...
We reported an updated database of MiCroKiTS 4.0 (http://microkit.biocuckoo.org) for proteins temporally and spatially localized in distinct subcellular positions including midbody, centrosome, kinetochore, telomere mitotic spindle during cell division/mitosis. The was from our previously developed MiCroKit 3.0, which contained 1489 mostly forming super-complexes at centrosome kinetochore seven eukaryotes. Since the apparatus are critical division, two were also integrated. From scientific...
Tomato fruit are sensitive to chilling injury (CI) during cold storage. Several factors have been discovered be involved in of tomato fruit. Plant hormones play an important regulatory role, however, the relationship between and N 6 -methyladenosine (m A) methylation transcripts plant hormone pathways has not reported yet. In order clarify complex mechanism m A on fruit, Nanopore direct RNA sequencing was employed. large number enzymes transcription were found regulation process injury,...