Abstract In the Brassicaceae, compatible pollen–pistil interactions result in pollen adhesion to stigma, while grains from unrelated plant species are largely ignored. There can also be an additional layer of recognition prevent self-fertilization, self-incompatibility response, whereby self distinguished nonself and rejected. This pathway is activated stigma involves ARM repeat–containing 1 (ARC1) protein, E3 ubiquitin ligase. a screen for ARC1-interacting proteins, we have identified...

ARC1 is a novel U-box protein required in the Brassica pistil for rejection of self-incompatible pollen; it functions downstream S receptor kinase (SRK). Here, we show that has E3 ubiquitin ligase activity and contains several motifs influence its subcellular localization. can shuttle between nucleus, cytosol, proteasome/COP9 signalosome (CSN) when expressed tobacco BY-2 suspension-cultured cells. However, localization to proteasome/CSN occurs only presence an active SRK. In pistil,...

Screening of a yeast two-hybrid library for proteins that interact with the kinase domain an S -locus receptor (SRK) resulted in isolation plant protein called ARC1 ( A rm R epeat C ontaining). This interaction was mediated by C-terminal region which five arm repeat units were identified. Using system and vitro binding assays, found to specifically domains from SRK-910 SRK-A14 but failed two different Arabidopsis receptor-like kinases. In addition, treatment phosphatase or use...

Self-incompatibility, the rejection of self pollen, is most widespread mechanism by which flowering plants prevent inbreeding. In Brassica , S receptor kinase (SRK) has been implicated in self-incompatibility response, but molecular mechanisms involving SRK are unknown. One putative downstream effector for ARC1, a protein that binds to domain. Here it shown suppression ARC1 messenger RNA levels self-incompatible napus W1 line correlated with partial breakdown self-incompatibility, resulting...

Abstract The Arabidopsis genome was searched to identify predicted proteins containing armadillo (ARM) repeats, a motif known mediate protein-protein interactions in number of different animal proteins. Using domain database predictions and models generated this study, 108 were identified that contained minimum two ARM repeats with the majority four eight repeats. Clustering analysis showed repeat could be divided into multiple groups wide differences their compositions organizations....

An S-receptor kinase (SRK) cDNA, SRK-910, from the active S-locus in a self-incompatible Brassica napus W1 line has been isolated and characterized. The SRK-910 gene is predominantly expressed pistils segregates with self-incompatibility phenotype an F2 population derived cross between self-compatible Westar line. Analysis of predicted amino acid sequence demonstrated that extracellular receptor domain highly homologous to glycoproteins, whereas cytoplasmic contains conserved acids present...

To determine potential targets of the S locus receptor kinase (SRK) during Brassica self-incompatibility response, a yeast two-hybrid library was screened with SRK-910 protein domain. Two thioredoxin-h-like clones, THL-1 and THL-2, were found to interact specifically domain not domains from Arabidopsis receptor-like kinases (RLK) RLK4 RLK5. The interaction between confirmed using coimmunoprecipitation experiments fusion proteins produced in Escherichia coli. has thioredoxin activity based on...

Abstract The Arabidopsis (Arabidopsis thaliana) genome encompasses multiple receptor kinase families with highly variable extracellular domains. Despite their large numbers, the various ligands and downstream interacting partners for these kinases have been deciphered only a few members. One such member, S-receptor kinase, is known to mediate self-incompatibility (SI) response in Brassica. has shown interact phosphorylate U-box/ARM-repeat-containing E3 ligase, ARC1, which, turn, acts as...

S locus glycoprotein (SLG) and receptor kinase (SRK) cDNAs were isolated from an allele present in a number of self-compatible Brassica napus lines. This A10 did not segregate with self-incompatibility crosses involving other self-incompatible B. The SLG-A10 cDNA was found to contain intact open reading frame predicted encode SLG protein sequence similarities those previously associated phenotypically strong reactions. transcripts detected the developing stigma at steady state levels even...

Transgenic mice carrying the gamma 2-crystallin promoter fused to coding region of bacterial lacZ gene were generated. The offspring three founder expressed high levels enzyme solely in central nuclear fiber cells lens as measured by an situ assay for detection beta-galactosidase activity. These results suggest that sequences between -759 +45 contain essential information required appropriate tissue-specific and temporal regulation mouse gene. In a broader context, this study also...

• The exocyst is a complex of eight proteins (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p and Exo84p) involved in tethering vesicles to the plasma membrane during regulated or polarized secretion. Here, plant was explored phylogenetic, expression, subcellular localization studies. Evolutionary relationships predicted subunits were examined complete genomes Arabidopsis thaliana, Oryza sativa, Populus trichocarpa Physcomitrella patens. Furthermore, detailed expression profiling A....

[In the Brassicaceae, targeted exocytosis to stigmatic papillar plasma membrane under compatible pollen grain is hypothesized be essential for hydration and tube penetration. In contrast, polarized secretion proposed inhibited in papillae during rejection of self-incompatible pollen. Using transmission electron microscopy (TEM), we performed a detailed time-course post-pollination events view cytological responses pollinations. For pollinations Arabidopsis thaliana lyrata, vesicle was...

Self-pollen rejection is an important reproductive regulator in flowering plants, and several different intercellular signaling systems have evolved to elicit this response. In the Brassicaceae, self-incompatibility system mediated by pollen S-locus Cys-Rich/S-locus Protein11 (SCR/SP11) ligand pistil S Receptor Kinase (SRK). While SCR/SP11-SRK recognition has been identified species across less known about conservation of SRK-activated cellular responses stigma, following self-pollen...

Abstract Flowering plants have evolved various strategies for avoiding self-pollen to drive genetic diversity. These include spatially separated sexual organs (herkogamy), timing differences between male pollen release and female pistil receptivity (dichogamy), rejection. Within the Brassicaceae, these outcrossing systems are evolutionary default state, many species display traits, including Arabidopsis lyrata. In contrast A. lyrata, closely related thaliana has lost traits thus represents...

ABSTRACT Transgenic mice carrying the diphtheria toxin A gene driven by mouse γ2-crystallin promoter sequences manifest microphthalmia due to ablation of fiber cells in ocular lens. Here we map events lens crossing animals hemizygous for construct with transgenic homozygous situ lacT reporter identical γ2 crystallin sequences. By comparing spatial distribution tacZ-expressing and profile γ-crystallin expression lenses normal microphthalmic offspring, contributions specific cell types...

Mate selection and maintenance of genetic diversity is crucial to successful reproduction species survival. Plants utilize self-incompatibility system as a barrier prevent self pollen from developing on the pistil, leading hybrid vigor diversity. In Brassica (canola, kale, broccoli), an allele-specific interaction between SCR/SP11 (S-locus cysteine rich protein/S locus protein 11) pistil S Receptor Kinase, results in activation SRK which recruits Arm Repeat Containing 1 (ARC1) E3 ligase...

Initial pollen-pistil interactions in the Brassicaceae are regulated by rapid communication between pollen grains and stigmatic papillae fundamentally important, as they first step toward successful fertilization. The goal of this study was to examine requirement exocyst subunits, which function docking secretory vesicles sites polarized secretion, context interactions. One subunit genes, EXO70A1, previously identified an essential factor stigma for acceptance compatible Arabidopsis...