A5091020854- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Restraint-Related Deaths
- Nitric Oxide and Endothelin Effects
- Monoclonal and Polyclonal Antibodies Research
- Autopsy Techniques and Outcomes
- Chemical Synthesis and Analysis
- Traumatic Ocular and Foreign Body Injuries
- Prenatal Screening and Diagnostics
- Hemoglobin structure and function
- Parvovirus B19 Infection Studies
- Neuroscience and Neuropharmacology Research
- Lipid Membrane Structure and Behavior
- Cancer therapeutics and mechanisms
- Heme Oxygenase-1 and Carbon Monoxide
- Synthesis and biological activity
- Injury Epidemiology and Prevention
- Advanced NMR Techniques and Applications
- Pancreatic function and diabetes
- Xenotransplantation and immune response
- Medical and Health Sciences Research
- Animal health and immunology
- Forensic Toxicology and Drug Analysis
- Metabolism and Genetic Disorders
- Renin-Angiotensin System Studies
CSL (Australia)
2013-2024
Kansas State University
2024
Saarland University
2015-2024
Folkwang University of the Arts
2021-2024
University of Siegen
2021-2024
Karlsruhe Institute of Technology
2024
Leipzig University
2010-2023
Rippl-Rónai Museum
2019-2023
University of Basel
2022
Behringwerke (Germany)
1989-2021
Patients undergoing liver transplantation for hepatitis B-related disease are prone to recurrence. The mainstay of prophylaxis has been passive immunotherapy with B immune globulin (HBIG). Antiviral therapy lamivudine proven effective in lowering virus (HBV) DNA and improving histology patients infection; its role against recurrence following is under investigation. Viral breakthrough resistance, however, a significant problem monotherapy either HBIG or lamivudine. efficacy combination...
ROS are a risk factor of several cardiovascular disorders and interfere with NO/soluble guanylyl cyclase/cyclic GMP (NO/sGC/cGMP) signaling through scavenging NO formation the strong oxidant peroxynitrite. Increased oxidative stress affects heme-containing receptor sGC by both decreasing its expression levels impairing NO-induced activation, making vasodilator therapy donors less effective. Here we show in vivo that related vascular disease states, including human diabetes mellitus, led to...
1. Soluble guanylyl cyclase (sGC) is the only proven receptor for ubiquitous biological messenger nitric oxide (NO) and intimately involved in many signal transduction pathways, most notably regulating vascular tone platelet function. sGC a heterodimeric (alpha/ss) protein that converts GTP to cyclic GMP; NO binds its prosthetic haem group. Here, we report discovery of novel activating compound, interaction with previously unrecognized regulatory site therapeutic implications. 2. Through...
Lysosomal cysteine proteinases of the papain family are involved in lysosomal bulk proteolysis, major histocompatibility complex class II mediated antigen presentation, prohormone processing, and extracellular matrix remodeling. Cathepsin L (CTSL) is a ubiquitously expressed representative papain-like proteinases. To investigate CTSL vivo functions, gene was inactivated by targeting embryonic stem cells. CTSL-deficient mice develop periodic hair loss epidermal hyperplasia, acanthosis,...
Soluble guanylate cyclase (sGC), a heterodimeric hemeprotein, is the only receptor for biological messenger nitric oxide (NO) identified to date and intimately involved in various signal transduction pathways. By using recently discovered NO- heme-independent sGC activator BAY 58-2667 novel cGMP reporter cell, we could distinguish between heme-containing heme-free an intact cellular system. Using these tools, invariant amino acids tyrosine 135 arginine 139 of β1-subunit as crucially...
Nitric oxide (NO) is an essential vasodilator. In vascular diseases, oxidative stress attenuates NO signaling by both chemical scavenging of free and oxidation downregulation its major intracellular receptor, the alphabeta heterodimeric heme-containing soluble guanylate cyclase (sGC). Oxidation can also induce loss heme sGC, as well responsiveness sGC to NO. activators such BAY 58-2667 bind oxidized/heme-free reactivate enzyme exert disease-specific vasodilation. Here, we show that...
Abstract Despite recent breakthroughs in the structural characterization of G‐protein‐coupled receptors (GPCRs), there is only sparse data on how GPCRs recognize larger peptide ligands. NMR spectroscopy, molecular modeling, and double‐cycle mutagenesis studies were integrated to obtain a model hormone neuropeptide Y (NPY) bound its human 2 receptor (Y R). Solid‐state measurements specific isotope‐labeled NPY complex with vitro folded R reconstituted into phospholipid bicelles provided...
Mucopolysaccharidosis VI (MPS VI) is a lysosomal storage disease with autosomal recessive inheritance caused by deficiency of the enzyme arylsulfatase B (ASB), which involved in degradation dermatan sulfate and chondroitin 4-sulfate. A MPS mouse model was generated targeted disruption ASB gene. Homozygous mutant animals exhibit elevated urinary secretion sulfate. They develop progressive symptoms resembling those humans. Around 4 weeks age facial dysmorphia becomes overt, long bones are...
Background and Purpose— The pathophysiology of hypoglycemia shares a common mechanism with cerebral ischemia, but so far, little is known regarding MRI humans hypoglycemia. Methods— We report patient left hemiparesis dysarthria associated blood glucose level 1.7 mmol/L. recovered completely after infusion. Results— initial diffusion-weighted imaging (DWI) showed increased signal intensities reduction apparent diffusion coefficient (ADC) values localized in the corpus callosum (splenium)...
A new catalytic method to synthesize the important anti-inflammatory agent naproxen [(S)-1] which has be used as (S) enantiomer, involves enantioselective decarboxylation of 6-methoxynaphth-2-yl derivative 2 2-cyanopropionic acid. Compound was stirred in THF at 15 °C with amounts chiral bases, abstracted carboxyl proton. After decarboxylation, reprotonation anion 6 afforded enantiomerically enriched nitrile 6, may hydrolyzed naproxen. variety bases were screened, and cinchona alkaloids found...
Specific antibodies directed against enterotoxic E. coli strains from the yolk of immunized hens were exposed in vitro to influence varying digestive phenomena like reduction pH and proteolytic digestion. The remaining antibody activity was tested a specific ELISA system. It could be shown that already within stomach considerable loss caused by lowering peptic cleavage can expected. A further is due effect pancreas proteases trypsin chymotrypsin. found protein rich solutions egg or...
The indications for penetrating keratoplasty have changed over the past several years. One hundred and eighty cases of performed in Department Ophthalmology, Naestved Centralsygehus, Denmark, from January 1984 to December 1993, were analysed. Overall pseudophakic bullous keratopathy was most common indication (28.3%). This followed by keratitis (13.9%), Fuchs' dystrophy regraft (11.1%), aphakic (10.0%) keratoconus (6.7%). Pseudophakic 1989 each year 1991 1993. Before (18.0%) (14.8%)....