A5100368158- Proteins in Food Systems
- Microencapsulation and Drying Processes
- Polysaccharides Composition and Applications
- Food composition and properties
- Lipid Membrane Structure and Behavior
- RNA Interference and Gene Delivery
- Radioactive element chemistry and processing
- Phytase and its Applications
- Polymer-Based Agricultural Enhancements
- Advanced biosensing and bioanalysis techniques
- Photochromic and Fluorescence Chemistry
- Hydrogels: synthesis, properties, applications
- Pickering emulsions and particle stabilization
- Supramolecular Self-Assembly in Materials
- Lanthanide and Transition Metal Complexes
- Pesticide and Herbicide Environmental Studies
- Cholesterol and Lipid Metabolism
- Advanced Polymer Synthesis and Characterization
- Cellular transport and secretion
- Click Chemistry and Applications
- Chemical Synthesis and Characterization
- Protein Hydrolysis and Bioactive Peptides
- Polyoxometalates: Synthesis and Applications
- Protein Structure and Dynamics
- Nanoplatforms for cancer theranostics
Shanghai Advanced Research Institute
2021-2025
Shanghai Zhangjiang Laboratory
2019-2025
Chinese Academy of Sciences
2010-2025
Ministry of Agriculture and Rural Affairs
2023-2025
Weifang People's Hospital
2025
Pulmonary Hospital of Lanzhou
2025
Ningxia Medical University
2024
South China Agricultural University
2024
Chengdu University of Traditional Chinese Medicine
2024
Nanjing Medical University
2024
Here, we report a β-galactosidase (β-Gal)-responsive photochromic fluorescent probe, NpG, that was designed to prebind human serum albumin (HSA) form the probe/protein hybrid, NpG@HSA. The formation of NpG@HSA led an increase in fluorescence emission (520 nm) corresponding binding naphthalimide unit with HSA. In addition, this enabled visualization spiropyran aqueous media. Our hybrid approach afforded unique imaging platform enhanced cell permeability and solubility capable visualizing...
In this study, an emulsion stabilized by soy protein isolate (SPI)-pectin (PC) complexes was prepared to investigate the effects of high-intensity ultrasound (HIU) treatment (150-600 W) on physicochemical properties, microstructure, and stability emulsions. The results found that treated at 450 W showed best index (ESI) (25.18 ± 1.24 min), lowest particle size (559.82 3.17 nm), largest ζ-potential absolute value (16.39 0.18 mV), highest adsorbed content (27.31%). Confocal laser scanning...
Significance Bunyaviruses are emerging zoonotic pathogens of public-health concern. Lack structures for proteins on the viral membrane (“envelope”) surface limits understanding entry. We describe atomic-level globular “head” envelope protein, glycoprotein N (Gn), from two members, severe fever with thrombocytopenia syndrome virus (SFTSV) and Rift Valley (RVFV), Phleboviruses genus in bunyavirus family, a structure SFTSV Gn bound neutralizing antibody Fab. The results show folded define...
The effects of high-intensity ultrasound on the physicochemical and gelling properties Litopenaeus vannamei (L. vannamei) myofibrillar protein (MP) were investigated. MP solutions subjected to treatment (power 100 W, 300 500 W). It was found that carbonyl free amino contents increased significantly with increasing power, accompanied by enhanced emulsification properties. increase radical content indicated induced oxidation MP. With it total sulfhydryl shrimp decreased, but surface...
In this study, hemp seed oil (HSO) emulsions stabilized with protein (HPI) were prepared and treated high intensity ultrasonic (HIU). The effects of different treatment powers (0, 150, 300, 450, 600 W) on the properties, microstructure stability investigated. HIU-treated showed improved emulsifying activity index index, reduced particle size, increased absolute values ζ-potential, extreme points these indices occurring at a power 450 W. Here, emulsion best dispersion smallest size in...
The influence of uranium (U) mining on the fungal diversity (FD) and communities (FC) structure was investigated in this work. Our results revealed that soil FC richness FD indicators obviously decreased due to U, such as Chao1, observed OTUs Shannon index (P<0.05). Moreover, abundances Mortierella, Gibberella, Tetracladium were notably reduced samples owing U activities In contrast, Cadophora, Pseudogymnoascus, Mucor, Sporormiella increased all after Furthermore, not only dramatically...
The torque of the bacterial flagellum is generated by rotor-stator interaction coupled with ion flow through channel in stator. Anchoring stator unit to peptidoglycan layer proper orientation around rotor believed be essential for smooth rotation flagellar motor. sodium-driven motor Vibrio composed PomA and PomB, thought fixed T-ring C-terminal periplasmic region PomB. Here, we report crystal structure a fragment PomB (PomBC) at 2.0-Å resolution, suggests conformational change N-terminal...
Obesity is a critical risk factor for hepatocellular carcinoma (HCC). However, it remains unknown whether inhibition of de novo lipid biosynthesis can suppress HCC. In this study, we blocked the sterol regulatory element‐binding protein (SREBP) pathway, one key determinants homeostasis, by ablating 78‐kDa cell‐surface glycoprotein or SREBP cleavage‐activating in hepatocytes, as well administering chemical compound called betulin. We found that either genetically pharmacologically inhibiting...
Novel cell-penetrating peptides self-assemble into ellipsoid-shape nanostructures which amplified the apoptotic stimuli by weakening VDAC1–HK-II interaction.
Herein, we report a protein-based hybridization strategy that exploits the host–guest chemistry of HSA (human serum albumin) to solubilize otherwise cell impermeable ONOO<sup>−</sup> fluorescent probe <bold>Pinkment-OAc</bold>.
MicroRNAs (miRNAs) are a new class of regulatory molecules implicated in type 2 diabetes, which is characterized by insulin resistance and hepatic glucose overproduction. We show that miRNA-451 (miR-451) elevated the liver tissues dietary genetic mouse models diabetes. Through an adenovirus-mediated gain- loss-of-function study, we found miR-451 negatively regulates gluconeogenesis blood levels normal mice identified glycerol kinase (Gyk) as direct target miR-451. demonstrate Gyk regulate...
The phase transition behaviors of a supported bilayer dipalmitoylphosphatidyl-choline (DPPC) have been systematically evaluated by in situ sum frequency generation (SFG) vibrational spectroscopy and atomic force microscopy (AFM). By using an asymmetric composed per-deuterated per-protonated monolayers, i.e., DPPC-d75/DPPC symmetric DPPC/DPPC, we were able to probe the molecular structural changes during process lipid SFG spectroscopy. It was found that DPPC is sequentially melted from top...
Microcapsule formulations have been highly desirable and widely developed for effective utilization of pesticides environmental pollution reduction. However, commercial traditional microcapsule λ-cyhalothrin (LC) were prepared by complicated synthesis procedures thereby specific organic solvents needed. In this work, LC was encapsulated into versatile, robust, biodegradable polydopamine (PDA) microcapsules self-polymerization dopamine. LC-loaded PDA characterized transmission electron...
Abstract Despite the widespread use of ionizable lipid nanoparticles (LNPs) in clinical applications for messenger RNA (mRNA) delivery, mRNA drug delivery system faces an efficient challenge screening LNPs. Traditional methods often require a substantial amount experimental time and incur high research development costs. To accelerate early stage LNPs, we propose TransLNP, transformer-based transfection prediction model designed to aid selection LNPs systems. TransLNP uses two types...
Niemann-Pick C1-like 1 (NPC1L1) is an essential protein for dietary cholesterol absorption. Nonsynonymous (NS) variants of NPC1L1 in humans have been suggested to associate with absorption variations. However, information concerning the characteristics and mechanism these uptake limited. In this study, we analyzed ability 19 reported NS identified from low absorbers. Among variants, L110F, R306C, A395V, G402S, T413M, R693C, R1214H, R1268H could partially mediate cellular were categorized as...
The use of phosphonic acids in the synthesis mixed-metal CeMn complexes has led to formation two phosphonate with unusual topologies: [Ce(2)Mn(6)O(6)(OH)(5)(t-BuPO(3))(6)(O(2)CMe)(3)] x 53 H(2)O (1 H(2)O) and [Ce(22)Mn(12)O(34)(MePO(3))(12)(O(2)CMe)(33)(OMe)(6)(NO(3))(H(2)O)(12)](n) (2). were both prepared from a reaction system containing Mn(O(2)CMe)(2) (NH(4))(2)[Ce(NO(3))(6)] similar procedures except for using different (tert-butylphosphonic acid methylphosphonic acid, respectively) as...
Flagellar motility is a key factor for bacterial survival and growth in fluctuating environments. The polar flagellum of marine bacterium, Vibrio alginolyticus , driven by sodium ion influx rotates approximately six times faster than the proton-driven motor Escherichia coli . basal body has two unique ring structures, T H ring. These structures are essential proper assembly stator unit into to stabilize motor. FlgT, which flagellar protein specific sp., required form both structures. Here,...