A5053210630- SARS-CoV-2 and COVID-19 Research
- Immune Cell Function and Interaction
- Influenza Virus Research Studies
- COVID-19 Clinical Research Studies
- Monoclonal and Polyclonal Antibodies Research
- IL-33, ST2, and ILC Pathways
- Eosinophilic Esophagitis
- Immunotherapy and Immune Responses
- Respiratory viral infections research
- Animal Virus Infections Studies
- Immune Response and Inflammation
- interferon and immune responses
- Hepatitis B Virus Studies
- MicroRNA in disease regulation
- Liver Disease Diagnosis and Treatment
- Asthma and respiratory diseases
- Animal Disease Management and Epidemiology
- Immune cells in cancer
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Air Quality Monitoring and Forecasting
- Inflammasome and immune disorders
- Organ Transplantation Techniques and Outcomes
- Bacillus and Francisella bacterial research
- Ferroptosis and cancer prognosis
Center for Excellence in Molecular Cell Science
2010-2024
University of Chinese Academy of Sciences
2019-2024
Chinese Academy of Sciences
2010-2024
Shanghai Public Health Clinical Center
2021
Biological E (India)
2021
Shanghai Medical College of Fudan University
2021
Institute of Cell Biology
2021
Institut Pasteur of Shanghai
2011-2012
Shanghai Institutes for Biological Sciences
2010-2011
Abstract Coronavirus disease 2019 (COVID-19), caused by the novel human coronavirus SARS-CoV-2, is currently a major threat to public health worldwide. The viral spike protein binds host receptor angiotensin-converting enzyme 2 (ACE2) via receptor-binding domain (RBD), and thus believed be target block entry. Both SARS-CoV-2 SARS-CoV share this mechanism. Here we functionally analyzed key amino acid residues located within binding motif of RBD that may interact with ACE2 available...
Hepatocyte ferroptosis promotes the pathogenesis and progression of liver fibrosis. Salvianolic acid B (Sal B) exerts antifibrotic effects. However, pharmacological mechanism target has not yet been fully elucidated. In this study, fibrosis was induced by CCl4 in wild-type mice hepatocyte-specific extracellular matrix protein 1 (Ecm1)-deficient mice, which were separately treated with Sal B, ferrostatin-1, sorafenib or cilengitide. Erastin- CCl4-induced hepatocyte models without Ecm1 gene...
Inflammatory bowel disease (IBD) comprises chronic relapsing disorders of the gastrointestinal tract characterized pathologically by intestinal inflammation and epithelial injury. Here, we uncover a function extracellular matrix protein 1 (ECM1) in promoting pathogenesis human mouse IBD. ECM1 was highly expressed macrophages, particularly tissue-infiltrated macrophages under inflammatory conditions, expression significantly induced during IBD progression. The macrophage-specific knockout...
Activation of TGFB (transforming growth factor β) promotes liver fibrosis by activating hepatic stellate cells (HSCs), but the mechanisms activation are not clear. We investigated role ECM1 (extracellular matrix protein 1), which interacts with extracellular and structural proteins, in mouse livers.We performed studies C57BL/6J mice (controls), ECM1-knockout (ECM1-KO) mice, hepatocyte-specific knockout EMC1 (ECM1Δhep). or soluble TGFBR2 (TGFB receptor 2) were expressed livers after injection...
Abstract Background The receptor-binding domain (RBD) variants of SARS-CoV-2 could impair antibody-mediated neutralization the virus by host immunity; thus, prospective surveillance antibody escape mutants and understanding evolution RBD are urgently needed. Methods Using single B cell cloning technology, we isolated characterized 93 RBD-specific antibodies from memory cells four COVID-19 convalescent individuals in early stage pandemic. Then, global alanine scanning with a panel 19 selected...
Abstract As influenza A viruses remain a major threat to human health worldwide, the discovery of broadly neutralizing monoclonal antibodies that recognize conserved epitopes would facilitate development antibody-based therapeutic strategies. Here we report V H 4-4-encoded mAb named 3E1 could neutralize H1 and H5 subtype in vitro protect mice against H1N1 H5N6 by inhibiting low pH-induced conformational rearrangement haemagglutinin (HA), hence blocking membrane fusion. The crystal structures...
Abstract Most structurally characterized broadly neutralizing antibodies (bnAbs) against influenza A viruses (IAVs) target the conserved conformational epitopes of hemagglutinin (HA). Here, we report a lineage naturally occurring human sharing same germline gene, V H 3-48/V K 1-12. These neutralize major circulating strains IAV in vitro and vivo mainly by binding contiguous epitope H3N2 HA, but H1N1 respectively. Our structural functional studies antibody 28-12 revealed that continuous amino...
Influenza A virus is highly variable and a major viral respiratory pathogen that can cause severe illness in humans. Therefore it important to induce sufficient immune response specific current strains heterosubtypic viruses with vaccines. In this study, we developed dual-promoter-based bivalent DNA vaccine encodes both hemagglutinin (HA) nucleoprotein (NP) proteins from pathogenic A/Chicken/Henan/12/2004 (H5N1) virus. Our results show the expression levels of HA NP genes dual-promoter...
Abstract Background Organisms have orchestrated coagulation and immune systems. Although a link between inflammation haemostasis has been reported in asthma, the interaction mechanism not completely elucidated. Here, we investigated direct mammalian Methods Mice were administered protease or antigens intranasally to induce airway with without thrombin inhibitors treatment. The effects of its on interleukin (IL)‐33 both vivo vitro. Peripheral blood mononuclear cells (PBMCs) plasma from asthma...
Induction of broadly neutralizing monoclonal antibodies (bNAbs) that bind to the viral envelope glycoproteins is a major goal hepatitis C virus (HCV) vaccine research. The study bNAbs arising in natural infection essential this endeavor. We generated human antibody, 8D6, recognizing E2 protein HCV isolated from chronic patient. This antibody shows activity, which covers pan-genotypic panel cell culture-derived virions (HCVcc). Functional and epitope analyses demonstrated 8D6 can block...
The Mulibrey (Muscle-liver-brain-eye) nanism caused by loss-of-function variants in TRIM37 gene is an autosomal recessive disorder characterized severe growth failure and constrictive pericarditis. These patients also suffer from respiratory infections, co-incident with increased mortality rate. Here, we revealed that were associated recurrent infection. Trim37 FINmajor (a representative variant of patients) knockout mice susceptible to influenza virus showed defects follicular helper T...
Introduction: MDM2 is known as the primary negative regulator of p53, promotes lung cancer fibrosis and injury through p53-dependent p53-independent pathways. However, mechanism by which influences pathogenesis asthma unknown. In this study, we investigated function in epithelial cells type 2 inflammation. Methods: We used II alveolar cell-specific heterozygous knockout Mdm2 mice to validate its function. Then papain-induced model was established, changes inflammation were observed measuring...