A5024644622- Pregnancy and preeclampsia studies
- Reproductive System and Pregnancy
- Genomics and Chromatin Dynamics
- Maternal and fetal healthcare
- Epigenetics and DNA Methylation
- Birth, Development, and Health
- Neonatal Respiratory Health Research
- Animal Virus Infections Studies
- Bone health and osteoporosis research
- Reproductive Biology and Fertility
- Ectopic Pregnancy Diagnosis and Management
- Pregnancy-related medical research
- COVID-19 Clinical Research Studies
- Cancer-related gene regulation
- Zebrafish Biomedical Research Applications
- Kruppel-like factors research
- Genetics and Neurodevelopmental Disorders
- MicroRNA in disease regulation
- Biomarkers in Disease Mechanisms
- CRISPR and Genetic Engineering
- SARS-CoV-2 and COVID-19 Research
- Pluripotent Stem Cells Research
- Preterm Birth and Chorioamnionitis
Albert Einstein College of Medicine
2019-2024
Chinese Academy of Sciences
2015-2024
Institute of Zoology
2015-2024
State Key Laboratory of Stem Cell and Reproductive Biology
2024
Center for Excellence in Molecular Cell Science
2020
Peking University Third Hospital
2017
Peking University
2017
University of Chinese Academy of Sciences
2017
Abstract Coronavirus disease 2019 (COVID-19), caused by the novel human coronavirus SARS-CoV-2, is currently a major threat to public health worldwide. The viral spike protein binds host receptor angiotensin-converting enzyme 2 (ACE2) via receptor-binding domain (RBD), and thus believed be target block entry. Both SARS-CoV-2 SARS-CoV share this mechanism. Here we functionally analyzed key amino acid residues located within binding motif of RBD that may interact with ACE2 available...
GnRH is expressed in first-trimester human placenta and increases cell invasion extravillous cytotrophoblasts (EVTs). Invasive phenotypes have been reported to be regulated by transcription factor activator protein 1 (AP-1) mesenchymal cadherin-11. The aim of our study was investigate the roles AP-1 components (c-FOS/c-JUN) cadherin-11 GnRH-induced EVT cells. Phosphorylated c-FOS phosphorylated c-JUN were detected column regions placental villi immunohistochemistry. treatment increased...
Preeclampsia, a multisystem syndrome occurring during mid- to late gestation in humans, is leading cause of maternal and perinatal morbidity mortality. Patients usually present with high circulating testosterone reduced estradiol production, but the mechanisms remain unclear. Revealing mechanism that modulating imbalance preeclampsia great value understanding disease. The placenta predominant source steroid hormone production gestation, we observed markedly increased 17β-HSD3...
Preeclampsia is a severe pregnancy-related disorder, and patients usually present with high circulating inflammatory factor levels excessive activation of the nuclear factor-κB (NF-κB) pathway. Administration aspirin (ASP) effective for preventing preeclampsia, thus, we propose that ASP might affect placental function by regulating NF-κB Systemic lipopolysaccharide (LPS) (20 μg/kg) was used to induce preeclampsia-like pregnant mouse model, low-dose (15.2 mg/kg) administrated. Here, report...
Ten-eleven translocation (Tet) enzymes promote DNA demethylation by oxidizing 5-methylcytosine. They are expressed during development and essential for mouse gastrulation. However, their postgastrulation functions not well established. We find that global or endothelial-specific loss of all three Tet immediately after gastrulation leads to reduced number hematopoietic stem progenitor cells (HSPCs) lethality in mid-gestation embryos. This is due defects specification HSPCs from endothelial...
We hypothesized that Runt-related transcription factor 2 (RUNX2), matrix metalloproteinase (MMP)2 and MMP9 are involved in basal gonadotrophin-releasing hormone (GnRH)-induced human extravillous trophoblast (EVT) cell invasion.Our finding indicates GnRH-induced RUNX2 expression enhances the invasive capacity of EVT cells by modulating MMP2 MMP9.GnRH is expressed first-trimester placenta exerts pro-invasive effects on vitro. regulates often associated with phenotypes.First-trimester (n = 9)...
Decidual NK (dNK) cells, identified as CD56 bright CD16 − CD3 , account for ~70% of lymphocytes within the uterine wall during early pregnancy. Accumulating evidence suggests that tight interactions between placental trophoblasts and dNK cells are critical trophoblast cell differentiation. However, underlying mechanism remains to be explored in detail. In present study, conditioned medium (CM) was collected from cultured primary human cells. Primary cytotrophoblasts (CTBs) or line HTR8/SVneo...
Decidual natural killer (dNK) cells are the most abundant immune at maternal-fetal interface during early pregnancy in both mice and humans, emerging single-cell transcriptomic studies have uncovered various human dNK subsets that disrupted patients experiencing recurrent loss (RPL) gestational stage, suggesting a connection between abnormal proportions or characteristics of RPL pathogenesis. However, functional mechanisms underlying this association remain unclear. Here, we established...
The Retinoid inducible nuclear factor (Rinf), also known as CXXC5, is a protein, but its functions in the context of chromatin are poorly defined. We find that mouse embryonic stem cells (mESCs), Rinf binds to and enriched at promoters enhancers Tet1, Tet2, pluripotency genes. Rinf-bound regions show significant overlapping occupancy factors Nanog, Oct4, Sox2, well Tet1 Tet2. found forms complex with Tet2 facilitates their proper recruitment regulatory Tet genes ESCs positively regulate...
DNA methylation is extensively reconfigured during development, but the functional significance and cell type-specific dependencies of demethylation in lineage specification remain poorly understood. Here, we demonstrate that developmental demethylation, driven by ten-eleven translocation 1/2/3 (TET1/2/3) enzymes, essential for establishment neural stem (NSC) identity gliogenic potential. We find loss all three TETs NSC dispensable induction neuronal differentiation critical astrocyte...
Objective: The signalling of the receptor tyrosine kinase Met is critical in promoting trophoblast cell invasion, and deficiency HGF/Met associated with preeclampsia. semaphorin family member semaphorin4D (sema4D) its Plexin-B1 have been reported to control tumour invasion by coupling Met. We hypothesized that sema4D/Plexin-B1 may promote activating Met, downregulation account for preeclamptic placenta. Methods: In this study, Erk activation expression normal placentas were comparably...