Abstract Preeclampsia, a relatively common pregnancy disorder, is major contributor to maternal mortality and morbidity worldwide. An elevation in microRNA-210 (miR-210) expression the placenta has been reported be associated with preeclampsia. Our bioinformatic analysis showed that thrombospondin type I domain containing 7A (THSD7A) predicted target for miR-210. The aim of this study was determine whether miR-210 involved preeclampsia through its targeting THSD7A human placental...

Significance The appropriate allocation of nutrients between the mother and fetus during pregnancy is predominantly governed by syncytiotrophoblast lining at outer surface placental villi. To date advantages such an extensively multinucleated cellular structure in substance exchange remains poorly understood. In this study, we discovered that differentiation trophoblasts toward syncytium triggers endocytosis strategy, macropinocytosis, to uptake large extracellular molecules. unique...

Little is known about the association between efficacy of neoadjuvant chemotherapy (NACT)/survival and dynamic change tumor immune environment (TIME) during treatment in epithelial ovarian cancer (EOC). This study investigated TIME landscape treatment-naive EOC tumors using multiplex immunofluorescence associated before after platinum-based NACT with prognosis 33 patients advanced EOC. significantly increased density CD8 + T cells ( P = 0.033), CD20 B 0.023), CD56 NK 0.041), PD-1 0.042),...

Cervical cancer is reportedly caused by the synergistic effects of persistent high-risk human papillomavirus (HPV) infection. microbiota represent a unique and dynamically changing microecological system that directly exposed to vagina. The relationship between HPV composition cervical microbiome has long been primary focus research.To determine specific differential florae throughout process development, in present study, 16S rRNA sequencing was combined with KEGG pathway enrichment...

Preeclampsia (PE), a placenta-associated pregnancy complication, is the leading cause of maternal and perinatal morbidity mortality. Met/Erk signaling inhibited in placentas patients with early-onset preeclampsia (E-PE), but underlying mechanisms remain elusive. In this study, expression modes Met endocytic vesicles normal preeclamptic were compared. Biotinylation internalization/recycling assays used to measure endocytosis under hypoxia normoxia HTR8/SVneo cells. addition, level Cbl,...

// Hong Qu 1, * , Yuling Chen 2, 3, Guangming Cao 1 Chongdong Liu Jiatong Xu 3 Haiteng Deng Zhenyu Zhang Department of Obstetrics & Gynecology, Beijing Chao-yang Hospital Affiliated to Capital Medical University, Beijing, China 2 Tsinghua University-Peking University Joint Center for Life Sciences, MOE Key Laboratory Bioinformatics, School These authors have contributed equally this work Correspondence to: Zhang, email: zhenyuzhang2000@163.com Deng, dht@mail.tsinghua.edu.cn Keywords:...

Background . Metformin has been reported to decrease insulin resistance and is associated with a lower risk of pregnancy-induced hypertension preeclampsia. It widely accepted that the placenta plays crucial role in development Our aim explore effect metformin on Study Design We examined control diet-fed (isocaloric diet) pregnant mice (CTRL group), fed high-fat diet (HF high-fat-diet-fed treated (HF-M group). The HF were six weeks before pregnancy establish preeclampsia-like model; then,...

Insufficient trophoblast invasion is associated with preeclampsia (PE) development. Retinol-binding protein 4 (RBP4) important for regulating cell differentiation, migration and invasion. The aim of the present study was to determine RBP4 expression function in human placenta examine underlying mechanisms. In study, determined serum samples from 35 pregnant women PE 30 healthy using enzyme-linked immunosorbent assays. Cell proliferation assessed by Counting Kit-8 assays, examined transwell...

Preeclampsia, a serious pregnancy-associated syndrome, is the leading cause of maternal and perinatal morbidity mortality. Significant exacerbation hypercoagulation status as well imbalanced steroid hormones have been reported in developed preeclampsia. However, it remains unclear whether two pathological changes are directly associated.Our proteomic analysis revealed significantly elevated SerpinF2/α2-antiplasmin level preeclampsia plasma. Measurement longitudinally gestational change...

To explore the role of lysophosphatidic acid receptor 1 (LPAR1) and its correlation with PI3K/AKT pathway in development intratumoral heterogeneity (ITH) human ovarian serous cystadenocarcinoma (OSC).Immunohistochemical staining was performed to detect LPAR1 expression matched primary recurrent lesions from same patients. Cell models ITH were established using limiting dilution methodology Transwell invasion/migration assays. cell silenced or upregulated lentiviral particles, biological...

Ovarian cancer is the 5th most common lethal gynecological malignancy with a 5-year survival rate of about 47% and localized stage diagnosis 15%, leading to 125,000 global deaths each year. Therefore, it urgent explore novel effective strategies for radical cure.Short hairpin RNA targeting Mucin16 (MUC16) gene was used establish MUC16 knockdown in ovarian cells. RT-PCR performed quantify expression mRNA, western blotting detect epithelial-mesenchymal transition-related proteins. Cell...

The present study aimed to investigate the role of FPR1 and downstream effectors such as NF-κB IL-6/8 in development cervical cancer.FPR1 protein expression was detected via immunohistochemical staining tissue microarrays containing cancer tissues from 185 patients. Following silencing SiHa cells using lentiviral siRNA delivery, biological characteristics tumor formation were evaluated vitro vivo, respectively. Phosphorylated levels by Western blotting, while IL-6 IL-8 secretion ELISA both...

Plateau pikas are seasonally breeding small herbivores that inhabit the meadow ecosystem of Qinghai-Tibetan Plateau. Testis regression in plateau begins early June, and male completely infertile, with a dramatically reduced testis size, late July. In this study, decreased germ cell number testes was first noted June. By only Sertoli cells spermatogonia remained. Interestingly, large gonocyte-like were observed July, per tubule increased significantly, most nuclei moved to clustered center...

This study tried to evaluate whether 8% polyethylene glycol (PEG) 6000 precipitation combined with differential ultracentrifugation (PPDU) was an efficient and practical method for the enrichment purification of extracellular vesicles (EVs) derived from culture supernatant human ovarian cancer cell line A2780 body fluids patients high-grade serous carcinoma (HGSC).PPDU used enrich purify EVs HSGC subclones high/low invasive capacity (named as A-H/A-L, respectively). Transmission electron...

Background. Vimentin, a cytoplasmic intermediate filament protein, has been recently identified to be prognostic biomarker in some cancers. However, the function of vimentin endometrial carcinoma (EC) remains unclear. Our study aimed at evaluating expression EC and preliminarily exploring role progression. Methods. In total, 341 patients who underwent surgical follow-up were enrolled retrospective study. Vimentin levels tissues analyzed using immunohistochemistry. Furthermore, (VIM) gene 547...

Objective This study aimed to investigate the potential role of galectin-3 (Gal-3) in pathogenesis fibrotic alterations ovarian endometriosis (OVE). Methods In this study, we collected ectopic endometrial tissues and eutopic from 31 OVE patients treated by laparoscopy, 23 non-OVE with leiomyoma or other benign diseases were used as control. Hematoxylin eosin (H&E) Masson’s trichrome staining utilized for histopathological assessment. The primary normal stromal cells (NESC), (ECSC),...

Cervical cancer (CC) is the 4th principal source of death in females with 604,000 new patients and 342,000 deaths 2020 worldwide. It has been extensively shown that circRNAs are involved regulating CC development. Nevertheless, function mechanisms hsa_circ_0004543 need to be clearly elucidated. Herein, expressions were compared between 40 paired paracancerous cancerous specimens from 6 cell lines a normal human cervical epithelial line based on qRT-PCR. Potential complementary binding sites...

Cervical cancer is the most common malignancy of female reproductive system. Dendritic cell (DC)-based immunological therapy a novel treatment for this cancer. DCs are specialized antigen-presenting cells (APCs) in human immune system, and they can activate T used tumor therapy. In study, we developed immunotherapeutic peptide by linking Mycobacterium tuberculosis (MTB) heat shock protein 70 (Hsp70) functional to extracellular domain FPR1, overexpressed cervical cancer, obtain an...

Preeclampsia (PE) is a hypertensive disorder of pregnancy. Early diagnosis PE currently contingent on regular prenatal physical examinations and may be facilitated by identification novel diagnostic markers. Transthyretin (TTR), also known as prealbumin, primarily responsible for maintaining the normal levels thyroxine retinol binding protein. The expression TTR lower in patients with severe compared healthy controls. Here, we examined suitability marker pregnant rats....