A5101461348- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Bacterial Infections and Vaccines
- CRISPR and Genetic Engineering
- Biochemical and Structural Characterization
- Plasma Applications and Diagnostics
- Bacterial Genetics and Biotechnology
- Mosquito-borne diseases and control
- Antimicrobial Peptides and Activities
- Streptococcal Infections and Treatments
- Pluripotent Stem Cells Research
- Infective Endocarditis Diagnosis and Management
- Immune Response and Inflammation
- Hippo pathway signaling and YAP/TAZ
- Bacterial Identification and Susceptibility Testing
- Pneumonia and Respiratory Infections
- Bacteriophages and microbial interactions
- Renal and related cancers
- Dermatology and Skin Diseases
- Viral Infections and Immunology Research
- Sirtuins and Resveratrol in Medicine
- Toxoplasma gondii Research Studies
- Microbial Natural Products and Biosynthesis
- Autophagy in Disease and Therapy
- Cellular Mechanics and Interactions
Army Medical University
2016-2025
Zhejiang Shuren University
2025
Daping Hospital
2024
Yellow River Institute of Hydraulic Research
2024
Beijing Biocytogen (China)
2024
The outbreak of 2019-novel coronavirus disease (COVID-19) that is caused by SARS-CoV-2 has spread rapidly in China, and developed to be a Public Health Emergency International Concern. However, no specific antiviral treatments or vaccines are available yet. This work aims share strategies candidate antigens develop safe effective against SARS-CoV-2.
Abstract Damage to vascular endothelial cells (VECs) is a critical hallmark of hemorrhagic diseases caused by dengue virus (DENV). However, the precise molecular event involved in DENV binding and infection VECs has yet be clarified. In this study, vimentin (55 kDa) was identified DENV-2 adsorption into VECs. This protein located on surface interacts with envelope domain III (EDIII). The expression level superficial not affected viral or siRNA interference, indicating that exists particular...
β-Lactam antibiotics are widely applied to treat infectious diseases. However, certain poor disease outcomes caused by β-lactams remain poorly understood. In this study, we have identified a cluster of lipoprotein-like genes ( lpl , sa2275 – sa2273 ) that is upregulated in the major clinically prevalent MRSA clones response subinhibitory concentrations β-lactam induction. The highlight work stimulate expression SarA, which directly binds promoter region and upregulates MRSA. Deletion...
Many viruses often have closely related yet antigenically distinct serotypes. An ideal vaccine against viral infections should induce a multivalent and protective immune response all Inspired by bacterial membrane vesicles (MVs) that carry different protein components, we constructed an agr locus deletion mutant of the Staphylococcus aureus strain (RN4220-Δagr) to reduce potential toxicity. Nanoscale derived from this (ΔagrMVs) at least four major components can deliver heterologous...
Staphylococcus aureus is a vital pathogen causing clinical infections. Capsules are important virulence factors for S. aureus. This study investigates the regulatory mechanisms underlying capsule production in Bacterial strains XN108 and Newman were used, combined approaches like RNA sequencing (RNA-seq), RT-qPCR, transmission electron microscopy (TEM), gene reporter, electrophoretic mobility shift assay (EMSA) performed to test role mechanism of WalK(S221P) mutation production. RNA-seq...
Abstract Background Dengue Virus (DENV) is prevalent in tropical and subtropical regions. With the projected climate change, traditional detection methods face limitations, there an urgent need for more accurate efficient diagnostic techniques. Objective The aim to integrate Recombinase-aided Amplification (recombinase-aided Amplification, RAA) Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) establish a special CRISPR-Cas system rapid, convenient, high sensitivity,...
Abstract Introduction: The ATP- and cGAMP-hydrolysing ecto-nucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3 or CD203c) is an extracellular enzyme which has been implicated in checkpoint regulation of innate immune responses. Analysis data Cancer Genome Atlas (TCGA) Cell Line Encyclopedia (CCLE) indicates elevated expression ENPP3 multiple solid tumors compared to normal tissues. Moreover, immunohistochemistry studies suggest that the on healthy epithelial lining polarized toward lumen...
Vancomycin-intermediate Staphylococcus aureus (VISA) strains have spread globally. We previously isolated an ST239 VISA (XN108) with a vancomycin MIC of 12 mg/L. The mechanism for XN108 resistance to was investigated in this study.Genome comparison performed characterize mutations that might contribute the phenotype. novel mutation WalK(S221P) identified and using allelic replacement experiments. Vancomycin susceptibilities, autolytic activities morphologies were examined....
Staphylococcus aureus is an important pathogen that produces abundant virulence factors, which cause various diseases burden human health worldwide. The stress response regulon called sigma factor B (SigB) a well-characterized global regulator involved in the regulation of S. virulence, pigmentation, and biofilm formation. However, regulatory network upon SigB incompletely described. Here, we identified novel substitution mutation, SigB(Q225P), contributed nonpigmented phenotype aureus....
infection poses a serious threat to public health, and antibiotic resistance has complicated the clinical treatment limited solutions available solve this problem. Cold atmospheric plasma (CAP) is promising strategy for microorganism inactivation. However, mechanisms of microbial inactivation or remain unclear. In study, we treated
The accessory gene regulator (agr) quorum-sensing system is an important global regulatory of Staphylococcus aureus and contributes to its pathogenicity. S. agr divided into four groups based on the amino acid polymorphisms AgrB, AgrD, AgrC. activation group-specific, resulting in variations activity pathogenicity among groups. Strains with divergent always have different phenotypes. In present report, we, respectively, exchanged a certain other three alleles assessed corresponding...
Abstract Staphylococcus aureus ( S. ), especially methicillin‐resistant (MRSA), causes wound infections, whose treatment remains a clinical challenge. Bacterium‐infected wounds often create acidic niches with pH 4.5–6.5. Endolysin LysSYL, which is derived from phage SYL, shows promise as an antistaphylococcal agent. However, endolysins generally exhibit instability and possess low bioavailability in microenvironments. Here, array of self‐assembling peptides designed, peptide L5 screened out...
Vancomycin-intermediate Staphylococcus aureus (VISA) is typically associated with a decline in virulence. We previously reported WalK(S221P) mutation that plays an important role mediating vancomycin resistance VISA XN108. Whether this implicated bacterial virulence remains unknown.This study aimed to investigate the effect of on and underlying mechanism effect.The influence its were explored using animal models, RNA-seq analysis, RT-qPCR, hemolytic assay, slide coagulase test, Western blot,...
Summary Pseudomonas aeruginosa is an important opportunistic human pathogen, which raises a worldwide concern for its increasing resistance. Nonthermal plasma, also called cold atmospheric plasma (CAP), alternative therapeutic approach clinical infectious diseases. However, the bacterial factors that affect CAP treatment remain unclear. The sterilization effect of portable device on different P. strains was investigated in this study. Results revealed can directly or indirectly kill...
Protein posttranslational modifications (PTMs) play important roles in regulating numerous biological functions of prokaryotic and eukaryotic organisms. Lysine succinylation (Ksucc) acetylation (Kac) are two PTMs that have been identified various bacterial species. However, the Ksucc Kac vancomycin-intermediate S. aureus (VISA) remain unclear. In this study, we systematically 3,260 sites 799 proteins 7,935 across 1,710 VISA strain XN108. Functional analyses revealed both were highly enriched...
Bacterial brain abscesses (BAs) are difficult to treat with conventional antibiotics. Thus, the development of alternative therapeutic strategies for BAs is high priority. Identifying virulence determinants that contribute BA formation induced by Staphylococcus aureus would improve effectiveness interventions this disease. In study, RT-qPCR was performed compare expression levels 42 putative S. strains Newman and XQ during murine formation, ear colonization, bacteremia. The alterations in 23...
The emergence of vancomycin-intermediate Staphylococcus aureus (VISA) has raised healthcare concerns worldwide. VISA is often associated with multiple genetic changes. However, the relative contributions these changes to phenotypes are incompletely defined. We have characterized XN108 vancomycin MIC 12 μg/ml. Genome comparison revealed that WalK(S221P), GraS(T136I), and RpoB(H481N) mutations possibly contributed phenotype XN108. In this study, above were stepwise cured, between its derivates...
Staphylococcus aureus remains a dangerous pathogen and poses great threat to public health worldwide. The prevalence of the S. clonotype is temporally geographically variable. genomic phenotypic characteristics isolates in Tianjin, which among four big municipalities China, are unclear. In present study, 201 nonduplicate isolates, including 70 methicillin-resistant (MRSA) 131 methicillin-susceptible (MSSA), were collected from 2015 2021 tertiary hospital Tianjin. Whole-genome sequencing was...
Abstract Vancomycin (VAN)‐intermediate Staphylococcus aureus (VISA) is a critical cause of VAN treatment failure worldwide. Multiple genetic changes are reportedly associated with VISA formation, whereas strains often present common phenotypes, such as reduced autolysis and thickened cell wall. However, how mutated genes lead to phenotypes remains unclear. Here, we show metabolism regulatory cascade (CcpA‐GlmS), whereby two‐component systems (TCSs) link the VISA. We found that ccpA deletion...
Staphylococcal enterotoxin B (SEB) has been demonstrated to be of importance in Staphylococcus aureus related diseases, such as atopic dermatitis (AD). Dysregulated apoptosis AD is remarkable, and SEB can induce various cell types. However, the mechanisms by which induces influences disease processes remain unclear. In this study, recombinant SEB-induced THP-1 monocyte was absence preliminary activation a time- dose-dependent manner. could up-regulate expression tumor necrosis factor alpha...
Staphylococcus aureus represents a major human pathogen that is frequently involved in polymicrobial infections. However, the prevalence and role of co-infectious microbes on pathogenesis fitness essentiality S. vivo remain largely unknown. In this study, we firstly performed retrospective surveillance 760 clinical samples revealed notable predominance co-infection with Acinetobacter baumannii. The high-density transposon mutant library coupled to insertion sequencing (Tn-Seq) further...
Growing evidence supports the notion that lipid metabolism is critical for embryonic stem cell (ESC) maintenance. Recently, α/β-hydrolase domain-containing (ABHD) proteins have emerged as novel pivotal regulators in synthesis or degradation while their functions ESCs not been investigated. In this study, we revealed role of ABHD11 ESC function using classical loss and gain experiments. Knockout Abhd11 hampered expansion differentiation, triggering autophagic flux apoptosis. contrast,...