A5101846576- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Antimicrobial Peptides and Activities
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Bacterial Identification and Susceptibility Testing
- Biochemical and Structural Characterization
- Streptococcal Infections and Treatments
- Infective Endocarditis Diagnosis and Management
- Salmonella and Campylobacter epidemiology
- Biomedical and Engineering Education
- Vibrio bacteria research studies
- Toxin Mechanisms and Immunotoxins
- Viral gastroenteritis research and epidemiology
- Advanced Neuroimaging Techniques and Applications
- Microbial Natural Products and Biosynthesis
- Antibiotic Resistance in Bacteria
- Orthopedic Infections and Treatments
- MRI in cancer diagnosis
- Autophagy in Disease and Therapy
- Microbial infections and disease research
- Monoclonal and Polyclonal Antibodies Research
- Neonatal and Maternal Infections
- Immunodeficiency and Autoimmune Disorders
Shanghai University
2024
Kunming University of Science and Technology
2024
Army Medical University
2014-2023
Fujian Medical University
2022
Union Hospital
2022
Xinqiao Hospital
2016
Shenyang Pharmaceutical University
2013
National Center for Nanoscience and Technology
2013
Louisiana State University Health Sciences Center New Orleans
2009
Czech Academy of Sciences, Institute of Microbiology
2006
ABSTRACT The emergence of Panton-Valentine leukocidin (PVL)-positive methicillin-resistant Staphylococcus aureus (MRSA) is a public health concern worldwide. PVL associated with community-associated MRSA and linked to skin soft tissue infections (SSTIs). However, genes have also been detected in care-associated (HA) isolates. diseases PVL-positive HA-MRSA isolates the distributions PVL-encoding bacteriophages not determined. In this study, total 259 strains isolated between 2009 2012 China...
The distribution of methicillin-resistant Staphylococcus aureus (MRSA) clones is dynamic and geographically unique. To understand the changing epidemiology MRSA infections in China, we performed a prospective, multicity surveillance study with molecular typing phenotypic analysis to determine association major prevalent their antimicrobial resistance profiles. A total 517 S. isolates collected between January 2009 March 2012 from six cities China were subjected antibiogram typing, including...
β-Lactam antibiotics are widely applied to treat infectious diseases. However, certain poor disease outcomes caused by β-lactams remain poorly understood. In this study, we have identified a cluster of lipoprotein-like genes ( lpl , sa2275 – sa2273 ) that is upregulated in the major clinically prevalent MRSA clones response subinhibitory concentrations β-lactam induction. The highlight work stimulate expression SarA, which directly binds promoter region and upregulates MRSA. Deletion...
Abstract Parkinson’s disease (PD) is characterized by the formation of Lewy body in dopaminergic neurons substantia nigra pars compacta (SNpc). Alpha-synuclein (α-syn) a major component body. Autophagy eliminates damaged organelles and abnormal aggregated proteins. Thioredoxin-1 (Trx-1) redox regulating protein plays roles protecting against neurotoxicity induced 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). However, relationship between Trx-1 α-syn PD still unknown. In present study,...
ObjectivesMethicillin-resistant Staphylococcus aureus (MRSA) infection is increasing and causing global concern. The mechanism of MRSA resistance to amikacin poorly understood. We report on the first matched-pair study reveal that phenotypic cell wall thickening associated with adaptive amikacin.
Gene duplication often provides selective advantages for the survival of microorganisms in adapting to varying environmental conditions. P. aeruginosa PAO1 possesses two seven-gene operons [phz1 (phzA1B1C1D1E1F1G1) and phz2 (phzA2B2C2D2E2F2G2)] that are involved biosynthesis phenazine-1-carboxylic acid its derivatives. Although highly homologous their functions well known, it is unclear how phz coordinate expressions maintain phenazine biosynthesis. By constructing single double deletion...
Vancomycin-intermediate Staphylococcus aureus (VISA) strains have spread globally. We previously isolated an ST239 VISA (XN108) with a vancomycin MIC of 12 mg/L. The mechanism for XN108 resistance to was investigated in this study.Genome comparison performed characterize mutations that might contribute the phenotype. novel mutation WalK(S221P) identified and using allelic replacement experiments. Vancomycin susceptibilities, autolytic activities morphologies were examined....
Our previous study showed that dengue virus 2 (DENV2) infection induces rearrangement of vimentin into dense structures at the perinuclear area. However, underlying mechanism this phenomenon is poorly characterized. In present work, we found and Ser71 phosphorylated display similar distributions in DENV2-infected cells. DENV2 also induced ROCK activation phosphorylation as progressed. Furthermore, by were blocked inhibitor Y-27632. addition, led to endoplasmic reticulum (ER) redistribution...
Sequence type (ST) 239 with SCCmec III methicillin-resistant Staphylococcus aureus (ST239-MRSA-III) is the most predominant multidrug-resistant clone in China. The subclone ST239-MRSA-III-t037 has been gradually replaced ST239-MRSA-III-t030 since 2000. Subclones are characterized by drug resistance profiles. However, mechanisms of clonal dynamics and determinants distinct remain poorly understood. In present study, 12 strains were collected from Chongqing, Guangzhou, Shanghai; these selected...
The accessory gene regulator (agr) quorum-sensing system is an important global regulatory of Staphylococcus aureus and contributes to its pathogenicity. S. agr divided into four groups based on the amino acid polymorphisms AgrB, AgrD, AgrC. activation group-specific, resulting in variations activity pathogenicity among groups. Strains with divergent always have different phenotypes. In present report, we, respectively, exchanged a certain other three alleles assessed corresponding...
Whole genome sequencing of a novel Pseudomonas aeruginosa temperate bacteriophage PaP3 has been completed. The contains 45 503 bp with GC content 52.1%, without more than 100 sequence hitting homologue in all sequenced phage genomes. A total 256 open reading frames (ORFs) are found the genome, and 71 ORFs predicated as coding (CDS). All CDS divided into two opposite direction groups, both groups meet at bidirectional terminator site locating near middle genome. is dsDNA 5'-protruded cohesive...
Staphylococcus aureus is an important pathogen that produces abundant virulence factors, which cause various diseases burden human health worldwide. The stress response regulon called sigma factor B (SigB) a well-characterized global regulator involved in the regulation of S. virulence, pigmentation, and biofilm formation. However, regulatory network upon SigB incompletely described. Here, we identified novel substitution mutation, SigB(Q225P), contributed nonpigmented phenotype aureus....
Methicillin-susceptible Staphylococcus aureus (MSSA) accounts for ∼40% of staphylococcal infections in China. However, the molecular characterization MSSA is not well described. In this study, 124 strains collected 2013 from a comprehensive teaching hospital Chongqing, Southwestern China, were subjected to antibiotics susceptibility testing and typing, including multilocus sequence protein A (spa) gene accessory regulator (agr) pulsed-field gel electrophoresis (PFGE) Panton-Valentine...
The obligate intracellular human pathogen Chlamydia trachomatis undergoes a complex developmental program involving transition between two forms: the infectious elementary body (EB), and rapidly dividing reticulate (RB). However, regulators controlling this development have not been identified. To uncover potential of transcription in C. , we screened genomic library for sequences encoding proteins that interact with RNA polymerase (RNAP). We report identification one such protein, CT663,...
Terminase proteins are responsible for DNA recognition and initiation of packaging in phages. We previously reported the genomic sequence a temperate Pseudomonas aeruginosa phage, PaP3, determined its precise integration site host bacterial chromosome. In this study, we present detailed functional identification terminase phage PaP3. The purified large subunit p03 was demonstrated to possess ATPase nuclease activities, as well ability bind specific when it is unassembled. addition, small...
Abstract Pathogenic bacteria could adjust gene expression to enable their survival in the distinct host environment. However, mechanism by which adapt environment is not well described. In this study, we demonstrated that nucleoside diphosphate kinase (Ndk) of Pseudomonas aeruginosa critical for adjusting bacterial virulence determinants during infection. Ndk was down-regulated pulmonary alveoli a mouse model acute pneumonia. Knockout ndk up-regulated transcription factor ExsA-mediated T3S...
Staphylococcus argenteus is a novel staphylococcal species (also considered as part of aureus complex) that infrequently reported on, and clinical S. infections are largely unstudied. Here, we report persistent recurrent hip joint infection case in which strain its small colony variants (SCVs) were successively isolated. We present features the two strains details their pathogenicity, explore factors induce SCVs formation course anti-infection therapy, reveal potential genetic mechanisms for...