A5100690820- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- SARS-CoV-2 and COVID-19 Research
- Infectious Diseases and Tuberculosis
- Viral gastroenteritis research and epidemiology
- Pneumocystis jirovecii pneumonia detection and treatment
- Diagnosis and treatment of tuberculosis
- Mosquito-borne diseases and control
- Animal Virus Infections Studies
- SARS-CoV-2 detection and testing
- Cellular transport and secretion
- COVID-19 Clinical Research Studies
- HIV/AIDS drug development and treatment
- Zoonotic diseases and public health
- Ideological and Political Education
- Animal Disease Management and Epidemiology
- Bacterial Infections and Vaccines
- Cancer therapeutics and mechanisms
- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Vaccine Coverage and Hesitancy
- Viral Infections and Outbreaks Research
- Bacillus and Francisella bacterial research
- Bacterial Genetics and Biotechnology
- Transgenic Plants and Applications
Shenyang Ninth People's Hospital
2024
Pfizer (United States)
2021-2024
Pearl River Community College
2023
National Vaccine and Serum Institute
2021
First Affiliated Hospital of Xi'an Jiaotong University
2017-2020
Hefei University of Technology
2020
Army Medical University
2013-2016
Fujian Medical University
2012
Guizhou Center for Disease Control and Prevention
2011
Institute of Microbiology
2011
Recently, a new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) lineage called B.1.1.7 (variant of concern: VOC 202012/01), which is reported to spread more efficiently and faster than other strains, emerged in the United Kingdom. This variant has an unusually large number mutations, with 10 amino acid changes spike (S) protein, raising concerns that its recognition by neutralizing antibodies may be affected. In this study, we tested SARS-CoV-2-S pseudoviruses bearing either...
Abstract Recently, a new SARS-CoV-2 lineage called B.1.1.7 has emerged in the United Kingdom that was reported to spread more efficiently than other strains. This variant an unusually large number of mutations with 10 amino acid changes spike protein, raising concerns its recognition by neutralizing antibodies may be affected. Here, we investigated SARS-CoV-2-S pseudoviruses bearing either Wuhan reference strain or protein sera 16 participants previously trial mRNA-based COVID-19 vaccine...
Abstract As SARS-CoV-2 evolves, increasing in potential for greater transmissibility and immune escape, updated vaccines are needed to boost adaptive immunity protect against COVID-19 caused by circulating strains. Here, we report features of the monovalent Omicron XBB.1.5-adapted BNT162b2 vaccine, which contains XBB.1.5-specific sequence changes, relative original backbone, encoded prefusion-stabilized spike protein (S(P2)). Biophysical characterization XBB.1.5 S(P2) demonstrated that it...
ABSTRACT As SARS-CoV-2 continues to evolve, increasing in its potential for greater transmissibility and immune escape, updated vaccines are needed boost adaptive immunity protect against COVID-19 caused by circulating strains. Here, we report features of the monovalent Omicron XBB.1.5-adapted BNT162b2 vaccine, which contains same mRNA backbone as original modified incorporation XBB.1.5-specific sequence changes encoded prefusion-stabilized spike protein (S(P2)). Biophysical characterization...
Our previous study showed that dengue virus 2 (DENV2) infection induces rearrangement of vimentin into dense structures at the perinuclear area. However, underlying mechanism this phenomenon is poorly characterized. In present work, we found and Ser71 phosphorylated display similar distributions in DENV2-infected cells. DENV2 also induced ROCK activation phosphorylation as progressed. Furthermore, by were blocked inhibitor Y-27632. addition, led to endoplasmic reticulum (ER) redistribution...
Abstract Background The emergence of multidrug-resistant tuberculosis (MDR-TB) poses a serious obstacle to global TB control programs. Methods We carried out prospective, randomized, multicenter study in China that was focused on the potential shorter regimen containing clofazimine (CFZ) for treatment MDR-TB. There were 135 MDR-TB cases met eligibility requirements and randomly stratified into either group or experimental group. Patients received an 18-month regimen, whereas patients...
ABSTRACT We constructed two recombinant Mycobacterium bovis BCG (rBCG) strains expressing ESAT-6 of tuberculosis , named rBCG-1 and rBCG-2. contained the gene linked to hsp60 expressed a fusion protein, while rBCG-2, with secretory sequence, could secret into culture medium. There was no evidence for increased virulence rBCG when we made comparison between them regard organ bacterial loads, lung histology, survival time. induced significantly higher specific antibody titers stronger cellular...
We carried out an investigation in five provinces China to assess treatment adherence and identify factors associated with insufficient tuberculosis (TB) patients mountainous, rural areas of China. In each the provinces, all counties > 80% mountainous area were stratified into three groups according their gross domestic product. stratum, one county was randomly sampled. Study subjects sampled from smear positive TB cases registered 2007 target counties. patients, village doctors, doctors...
Purpose. XpertMycobacterium tuberculosis/rifampicin (Xpert MTB/RIF) assay has been endorsed by the World Health Organization (WHO) for diagnosis of extrapulmonary tuberculosis (EPTB), while sensitivity and specificity have not fully evaluated. We aimed to evaluate performance Xpert MTB/RIF in detection different specimens. Methods. A total 420 nonrespiratory specimens were detected with acid-fast bacilli (AFB) smear microscopy, solid culture, conventional drug susceptibility testing (DST)...
Lassa virus (LASV) is a rodent-borne arenavirus circulating in West African regions that causes fever (LF). LF normally asymptomatic at the initial infection stage, but can progress to severe disease with multiorgan collapse and hemorrhagic fever. To date, therapeutic choices are limited, there no approved vaccine for avoiding LASV infection. Adenoviral vector-based vaccines represent an effective countermeasure against because of their safety adequate immunogenicity, as demonstrated use...
<i>Objective:</i> The pathogenesis of dengue virus (DV) has not been completely clarified. Rab8 regulates vesicular traffic from Golgi to plasma membrane where DV is matured and then delivered by exocytosis. In this study, involvement in serotype 2 (DV2) infection was investigated HpeG2 cells. <i>Methods:</i> Distributions DV2, the number cells were observed immunostaining. HepG2<sup>Rab8AM</sup> HepG2<sup>Rab8DN</sup> constructed stably...
Objectives The prevalence of drug-resistant TB in Shaanxi Province is higher than other areas. This study was aimed to investigate the genetic diversity and epidemiology Mycobacterium tuberculosis clinical strains Province, China. Methods From January December 2016, a total 298 isolates from smear-positive pulmonary patients were genotyped by Mcspoligotyping 15-locus VNTR. Results We found that Beijing family most prominent family(81.54%, 243/298). Other included T family(9.06%, 27/298), U...
Objective To investigate the current status of health care-seeking delay and treatment tuberculosis (TB) patients influencing factors in two counties Hubei Province, provide evidence for early detection TB patients. Methods Cross-sectional survey was conducted through face to interview collection patient's medical record information, outpatient inpatient records hospitals univariate analysis logistic regression identify independently associated with delay. Results The median care seeking...
The ESX-1 secretion system plays a critical role in the virulence of Mycobacterium tuberculosis. secreted protein EspB is cleaved close to its C-terminus during and necessary for inhibiting phagosome maturation. In this study, gene M. tuberculosis H37Rv was cloned into expression vector pET21a(+) effectively expressed Escherichia coli BL21(DE3). fusion existed as soluble form cell lysate first purified by column packed with Ni-NTA resin then DEAE-Sepharose Fast Flow matrix. Using immunize...
Background: We assessed the safety and immunogenicity of heterologous schedules with a recombinant adenovirus type-5-vectored COVID-19 vaccine (Convidecia) protein-subunit-based (ZF2001). Methods: did randomised, observer-blinded, placebo-controlled trial in healthy adults, vaccinated single dose Convidecia 28 days before screening, no history SARS-CoV-2 infection. Participants were randomly assigned (2:1) to receive either ZF001 (vaccine group) or trivalent inactivated influenza (TIV)...
To analyze the application effect of Adherence Rating Score (ARS) scale in treatment patients with tuberculosis.A total 1 349 tuberculosis from November 2013 to May 2014 ten counties Guizhou province were selected. Before treatment, ARS was applied. According data related literature on compliance during follow-up visits late and June (the reviewed August), correlation evaluation score actual analyzed.There 705 cases high but 249 low when ≥4, while 213 182 <4 May. The ≥4 significantly...