A5100433089- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- Cholinesterase and Neurodegenerative Diseases
- Enzyme Catalysis and Immobilization
- Natural product bioactivities and synthesis
- Phytochemistry and Biological Activities
- Synthesis and biological activity
- Computational Drug Discovery Methods
- Carbohydrate Chemistry and Synthesis
- Polysaccharides and Plant Cell Walls
- Chemical synthesis and alkaloids
- Glycosylation and Glycoproteins Research
- Plant biochemistry and biosynthesis
- Pesticide Exposure and Toxicity
- Agriculture, Soil, Plant Science
- Ginseng Biological Effects and Applications
- Plant Pathogens and Fungal Diseases
- Marine Sponges and Natural Products
- Plant tissue culture and regeneration
- Synthetic Organic Chemistry Methods
- Genomics and Phylogenetic Studies
- Enzyme Production and Characterization
- Phytochemical compounds biological activities
- Plant Stress Responses and Tolerance
- Essential Oils and Antimicrobial Activity
Hebei University
2013-2024
Shanghai First Maternity and Infant Hospital
2020-2024
Shanghai Institute of Materia Medica
2024
University of Chinese Academy of Sciences
2024
Ningbo University
2021-2024
Chinese Academy of Sciences
2009-2023
Peking University
2008-2023
Shenyang Pharmaceutical University
2023
Southern University of Science and Technology
2023
Chinese Academy of Agricultural Sciences
2017-2023
Although imine reductases (IREDs) are emerging as attractive reductive aminases (RedAms), their substrate scope is still narrow, and rational engineering rare. Focusing on hydrogen bond reorganization cavity expansion, a concise strategy combining design, combinatorial active-site saturation test (CAST), thermostability was designed, that transformed the weakly active IR-G36 into variant M5 with superior performance for synthesis of (R)-3-benzylamino-1-Boc-piperidine, 4193-fold improvement...
Mycotoxin contamination causes disease and death in both humans animals. Monascus Red, produced by purpureus, is used as a food colorant. However, its application limited of the nephrotoxin citrinin, which also fungus. Suppressing citrinin production genetic engineering difficult polykaryotic fungus such M. purpureus. Hence, we developed CRISPR/Cas system to delete large genomic fragments fungi. Protoplast preparation regeneration were optimized, dual-plasmid was designed enable deletion...
N-Glycosylation represents an essential type of posttranslational modification for proteins. However, deciphering the functions N-glycosylation remains a challenge due to lack analytical and biochemical methods accurately differentiate protein glycoforms with various intact glycans. Here we report our synthesis evaluation homogeneously glycosylated interleukin-17A (IL-17A), based on synthetic approach combining solid-phase (glyco)peptides, chemoenzymatic glycan segments, chemical ligations....
Evodiamine (Evo), a major alkaloid compound isolated from the dry unripened fruit of Evodia fructus, has wide range pharmacological activities. The present study sought to explore neuroprotective effects Evo in l-glutamate (l-Glu)-induced apoptosis HT22 cells, and d-galactose aluminum trichloride-developed Alzheimer’s disease (AD) mouse model. significantly enhanced cell viability, inhibited accumulation reactive oxygen species, ameliorated mitochondrial function, increased B-cell...
Ganoderma lucidum polysaccharides (GLP) exhibited excellent immunomodulatory activity. Unfortunately, the structure and activity of GLP are still unclear. was separated into two fractions [high Mw Restriction Fragment Length Polymorphism (RGLP) low EGLP] using 10 kDa cut-off ultrafiltration membrane. Although RGLP content in GLP, significantly higher than that EGLP. Moreover, further via Sephacryl column to obtain RGLP-1 showed best macrophage RAW264.7 model. Structural analysis revealed...
Tiger nut (Cyperus esculentus L.) has recently attracted increasing interest from scientific and technological communities because of its potential for serving as additional source food, oil, feed. The present study reports morphology biochemical characterization 42 tiger accessions collected China other counties performed in the 2020 2021 growing seasons at Nongan, Jilin Province. Assessment variability 14 agronomic traits including plant height, maturation, leaf width, tilling number,...
In this study, engineered imine reductases (IREDs) of IRED M5, originally from Actinoalloteichus hymeniacidonis , were obtained through structure-guided semi-rational design.
Liquiritigenin (LIQ) is a dihydroflavonone monomer compound with planar ring structure that exhibits potent anti-inflammatory activity. The post-translational modifications (PTMs) of histones are closely associated inflammatory diseases. To explore the relationships between effects and epigenetic regulatory mechanisms LIQ, we optimized super stable isotope labeling by amino acids in cell culture (super-SILAC) method combined stimulation strategy. Moreover, evaluated identification coverage...
<h3>Background and aims</h3> Gastrokine 1 (GKN1) is a stomach-specific protein that normally expressed in gastric mucosa but not primary tumours cell lines. Based on this evidence, it was presumed GKN1 might play role cancer development; however, its function molecular mechanism are clear. A systematic study initiated combined multiple approaches to define the of cells. <h3>Method</h3> Proteomics, western blotting immunohistochemistry were used measure expression level GKN1. Western with...
Prenylated flavonoids and isoflavonoids are widely distributed throughout the plant kingdom, with many biological effects. Psoralea corylifolia, which contains kinds of prenylated components, has been used as a medicinal in Asia India for thousands years. The goal this study was to characterize components P. corylifolia using liquid chromatography diode-array detection quadrupole time-of-flight mass spectrometry (LC-DAD/Q-TOF-MS) method, elucidate fragmentation behavior different prenyl...
ABSTRACT Heterologous expression of β‐glucosidase is one the approaches to enhance efficiency fungal cellulase preparations. It has been reported that N ‐glycosylation affects structure framework, function and stability proteins. In this study, a from Aspergillus terreus (GenBank: XP_001216552, BglS) was heterologously expressed in Pichia pastoris Trichoderma reesei . The four asparagine residues were all linked with high‐mannose‐type oligosaccharides P. , whereas only N224 carried glycan T....
Five pairs of isoquinoline alkaloid enantiomers, mucroniferanines A-E (1-5), two inseparable epimeric pairs, F and G (6, 7), 10 known alkaloids (8-17) were obtained from Corydalis mucronifera. The structures characterized using spectroscopic data analysis, the absolute configurations established by ECD X-ray analysis. new compounds except for 3 possess a rare 9-methyl group in alkaloids, 2 benzo[1,2-d:3,4-d]bis[1,3]dioxole moieties. It is first report stereoisomerism involving...
The purpose of the current study was to evaluate optimal compatibility proportion 18α-Glycyrrhizic acid (18α-GA) and 18β-Glycyrrhizic (18β-GA) against carbon tetrachloride (CCl4)-induced hepatic damage in rats, further explored underlying mechanism. Rats were injected with CCl4 (0.1%, 0.3 ml/kg) once a week orally administrated different proportions 18α-, 18β-GA daily for 4 weeks. then sacrificed blood samples collected biochemical assay. Liver tissues assessed histologically severity liver...
Since imine reductases (IREDs) were reported to catalyze the reductive amination reactions, they became particularly attractive for producing chiral amines. Though diverse ketones and aldehydes have been proved be excellent substrates of IREDs, bulky amines rarely transformed. Here we report usage an Increasing-Molecule-Volume-Screening identify a group IREDs (IR-G02, 21, 35) competent accepting amine substrates. IR-G02 shows substrate scope, which is applied synthesize over 135 molecules as...