A5090282706- Circular RNAs in diseases
- FOXO transcription factor regulation
- RNA modifications and cancer
- Cancer Cells and Metastasis
- Bacterial biofilms and quorum sensing
- Metabolism, Diabetes, and Cancer
- Cancer Mechanisms and Therapy
- Cancer-related molecular mechanisms research
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Antibiotic Resistance in Bacteria
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- MicroRNA in disease regulation
- Genomics and Phylogenetic Studies
- Pancreatic function and diabetes
- Mesenchymal stem cell research
- Cancer Research and Treatments
- High-Velocity Impact and Material Behavior
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- CAR-T cell therapy research
- Mechanical Behavior of Composites
- Structural Response to Dynamic Loads
- Cancer-related Molecular Pathways
Xinqiao Hospital
2015-2024
Army Medical University
2015-2024
Chongqing Medical University
2023
Dalian Medical University
2023
Second Affiliated Hospital of Chongqing Medical University
2023
Nanjing University of Science and Technology
2020-2022
Army Medical College
2020
Texas A&M University
2018-2019
Yangtze University
2019
Central Texas Veterans Health Care System
2011-2014
Abstract Background Aberrant expression of circular RNAs contributes to the initiation and progression cancers, but underlying mechanism remains elusive. Methods RNA-seq qRT-PCR were performed screen differential expressed circRNAs between gastric cancer tissues adjacent normal tissues. Candidate circRNA (circMRPS35) was screened out validated by qRT-PCR. Cell proliferation invasion ability determined CCK-8 cell assays. RNA-seq, GO-pathway, RNA pull-down ChIRP further applied search for...
Dysregulation of blood glucose and triglycerides are the major characteristics type 2 diabetes mellitus. We sought to identify mechanisms regulating lipid homeostasis. Cell-based studies established that Foxo forkhead transcription factors Forkhead box O (Foxo)-1, Foxo3, Foxo4 inactivated by insulin via a phosphatidylinositol 3-kinase/Akt-dependent pathway, but role in liver nutrient metabolism is incompletely understood. In this study, we used Cre/LoxP genetic approach delete Foxo1, genes...
Heart failure is a leading cause of morbidity and mortality in the USA closely associated with diabetes mellitus. The molecular link between mellitus heart incompletely understood. We recently demonstrated that insulin receptor substrates 1, 2 (IRS1, 2) are key components signaling loss IRS1 IRS2 mediates resistance, resulting metabolic dysregulation failure, which downstream Akt inactivation turn activation forkhead transcription factor Foxo1.To determine role Foxo1 control resistance...
Dysregulation of hepatic glucose production (HGP) serves as a major underlying mechanism for the pathogenesis type 2 diabetes. The pancreatic hormone glucagon increases and insulin suppresses HGP, controlling blood homeostasis. forkhead transcription factor Foxo1 promotes HGP through increasing expression genes encoding rate-limiting enzymes responsible gluconeogenesis. We previously established that by Akt-mediated phosphorylation at Ser256 in human hepatocytes. In this study, we found...
Elastase LasB, an important extracellular virulence factor, is shown to play role in the pathogenicity of Pseudomonas aeruginosa during host infection. However, LasB life cycle P. not completely understood. This report focuses on impact biofilm formation PAO1. Here, we reported that lasB deletion mutant (ΔlasB) displayed significantly decreased bacterial attachment, microcolony formation, and matrix linkage associated with biosynthesis rhamnolipids compared PAO1 complementary strain...
Pseudomonas aeruginosa is a prevalent opportunistic pathogen that causes fatal infections in immunocompromised individuals. Quorum sensing (QS) cell-to-cell communication process controls virulence gene expression and biofilm formation P. aeruginosa. Here, the QS systems relevant traits clinical isolates were characterized. Eleven out of ninety-four exhibited biofilm-deficient phenotype. Two isolates, one from blood pleural effusion, appeared to carry same mutation lasR. These two differed...
BACKGROUND:Metformin is a widely used biguanide drug for the treatment of type 2 diabetes. It has been revaluated as potential anti-cancer with promising activity in various tumors. However, precise mechanisms underlying suppression cancer cells by metformin remain not well understood. MATERIAL AND METHODS:In this study, human renal cell carcinoma line ACHN was to investigate anti-proliferation effect metformin. A counting kit-8 assay detect viability. The cycle distribution and apoptosis...
The purpose of this study was to investigate the therapeutic effects hyperoside (Hyp) on glomerulosclerosis in diabetic nephropathy and its underlying mechanisms. Blood glucose, kidney mass, renal function mice were measured. Renal morphology observed using hematoxylin eosin, periodic acid - Schiff's, Masson's trichrome stain. Fibronectin (FN) collagen IV (COL IV) determined by Western blot immunohistochemical studies. Matrix metalloproteinases (MMP)-2 -9 tissue inhibitors metalloproteinase...
Pseudomonas aeruginosa is a virulent bacterium that secretes variety of virulence factors aid in establishing infections individuals. Allicin, derived from garlic, has been shown to inhibit factor production and biofilm formation P. aeruginosa. However, the mechanisms underlying allicin-mediated regulation remain unclear. In this study, we investigated possible The results showed allicin attenuates virulence-associated factors, such as elastase, pyocyanin, pyoverdine, rhamnolipids, by...
Abstract The transcription factor forkhead box O1 (FoxO1) is a key mediator in the insulin signaling pathway and controls multiple physiological functions, including hepatic glucose production (HGP) pancreatic β-cell function. We previously demonstrated that S256 human FOXO1 (FOXO1-S256), equivalent to S253 mouse FoxO1 (FoxO1-S253), phosphorylation site mediating effect of as target protein kinase B on suppression activity expression genes responsible for gluconeogenesis. Here, we...
Whole genome sequencing of a novel Pseudomonas aeruginosa temperate bacteriophage PaP3 has been completed. The contains 45 503 bp with GC content 52.1%, without more than 100 sequence hitting homologue in all sequenced phage genomes. A total 256 open reading frames (ORFs) are found the genome, and 71 ORFs predicated as coding (CDS). All CDS divided into two opposite direction groups, both groups meet at bidirectional terminator site locating near middle genome. is dsDNA 5'-protruded cohesive...
The renin-angiotensin system is a major determinant of blood pressure regulation. It consists cascade enzymatic reactions involving 3 components: angiotensinogen, renin, and angiotensin-converting enzyme, which generate angiotensin II as biologically active product. Angiotensinogen largely produced in the liver, acting circulating system, exerts acute hemodynamic effects on How expression angiotensinogen regulated not completely understood. Here, we hypothesize that by forkhead transcription...
Terminase proteins are responsible for DNA recognition and initiation of packaging in phages. We previously reported the genomic sequence a temperate Pseudomonas aeruginosa phage, PaP3, determined its precise integration site host bacterial chromosome. In this study, we present detailed functional identification terminase phage PaP3. The purified large subunit p03 was demonstrated to possess ATPase nuclease activities, as well ability bind specific when it is unassembled. addition, small...
Abstract Pathogenic bacteria could adjust gene expression to enable their survival in the distinct host environment. However, mechanism by which adapt environment is not well described. In this study, we demonstrated that nucleoside diphosphate kinase (Ndk) of Pseudomonas aeruginosa critical for adjusting bacterial virulence determinants during infection. Ndk was down-regulated pulmonary alveoli a mouse model acute pneumonia. Knockout ndk up-regulated transcription factor ExsA-mediated T3S...
Berberine (BBR), an isoquinoline alkaloid isolated from Rhizoma coptidis, is reported to possess antiviral activity. Our previous study has shown that BBR alleviates coxsackievirus B3 (CVB3) replication in HeLa cells. However, the anti-CVB3 activity of still unclear vivo. In this study, we explored effect on CVB3-induced viral myocarditis mice. These results demonstrated beneficial alleviating vivo, which sheds new light utility as a therapeutic strategy against myocarditis.
Pseudomonas aeruginosa biofilms employ a variety of strategies to hijack the host immune defense system achieve chronic infection. However, bacterial components that are involved in this process not yet fully understood. PcrV, needle tip protein P. type III secretion (T3SS), was downregulated during biofilm The impaired expression pcrV gene is associated with attenuated activation and an increased percentage M2 macrophages following Treatment exogenous PcrV produced from Escherichia coli...
is one of the most common nosocomial pathogens worldwide, known for its virulence, drug resistance, and elaborate sensor-response network. The primary challenge encountered by during initial stages infection immune clearance arising from host. resident macrophages barrier organs serve as frontline defense against these pathogens. Central to our understanding mechanism which bacteria modify their behavior circumvent macrophage-mediated clearance, ensuring persistence colonization. To...
BACKGROUND At present, the treatment of coxsackievirus-induced myocarditis remains difficult. Berberine (BBR), an isoquinoline alkaloid isolated from traditional medicine herbs, exhibits significant anti-viral efficacy against various viruses. However, underlying mechanism by which BBR controls CVB3 infection has not yet been reported. The purpose this study was to investigate and its mechanism. MATERIAL AND METHODS In our experiments, protein levels VP1 MAPKs signal pathway were measured...
Intestinal ischemic reperfusion (I/R) can cause dysfunction of the intestinal mucosal barrier; however, mechanism barrier caused by I/R remains unclear. In this study, using epithelial cells under anaerobic cultivation and an in vivo rat model, we found that hypoxia increased expression BMP2/4 upregulated BMP type Ia receptor II expression. We also exogenous activate ERK AKT signaling pathways small intestine (IEC-6) cells, thereby activating NF-<mml:math...