A5002881873- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Circular RNAs in diseases
- RNA regulation and disease
- Endoplasmic Reticulum Stress and Disease
- Cancer Mechanisms and Therapy
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Hearing, Cochlea, Tinnitus, Genetics
- MicroRNA in disease regulation
- Ubiquitin and proteasome pathways
- Cancer Genomics and Diagnostics
- Medical Imaging and Analysis
- Vibrio bacteria research studies
- Inflammasome and immune disorders
- Liver Disease Diagnosis and Treatment
- RNA and protein synthesis mechanisms
- Bacterial biofilms and quorum sensing
- Noise Effects and Management
- Cancer, Lipids, and Metabolism
- Lipid metabolism and disorders
- Chromosomal and Genetic Variations
- Pluripotent Stem Cells Research
- Acute Myeloid Leukemia Research
Army Medical University
2015-2025
Xinqiao Hospital
2015-2025
Chengdu University of Traditional Chinese Medicine
2025
North Sichuan Medical University
2023-2024
Anhui University of Traditional Chinese Medicine
2023-2024
Xuzhou Medical College
2024
Southeast University
2016-2023
First Affiliated Hospital of Henan University
2023
Sichuan University
2022
National University of Defense Technology
2022
Abstract Background Aberrant expression of circular RNAs contributes to the initiation and progression cancers, but underlying mechanism remains elusive. Methods RNA-seq qRT-PCR were performed screen differential expressed circRNAs between gastric cancer tissues adjacent normal tissues. Candidate circRNA (circMRPS35) was screened out validated by qRT-PCR. Cell proliferation invasion ability determined CCK-8 cell assays. RNA-seq, GO-pathway, RNA pull-down ChIRP further applied search for...
Polycomb repressive complex two (PRC2) has been implicated in embryonic stem (ES) cell pluripotency; however, the mechanistic roles of this are unclear. It was assumed that ES cells contain PRC2 with same subunit composition as identified HeLa and Drosophila embryos. Here, we report mouse contains at least three additional subunits: JARID2, MTF2, a novel protein denoted esPRC2p48. esPRC2p48 highly expressed compared to differentiated cells. Importantly, knockdowns or alter level...
Significance Polycomb repressive complex 1 (PRC1) represents an important epigenetic regulator, which exerts its effect on gene expression via histone H2A ubiquitination (ubH2A). We developed a conditional Usp16 knockout mouse model and demonstrated that is indispensable for hematopoiesis hematopoietic stem cell (HSC) lineage commitment. identified to be deubiquitinase counterbalances the PRC1 ubiquitin ligase control ubH2A level in system. Conditional deletion led altered of many regulators...
Abstract Background Long‐term non‐traumatic noise exposure, such as heavy traffic noise, can elicit emotional disorders in humans. However, the underlying neural substrate is still poorly understood. Methods We exposed mice to moderate white for 28 days induce anxiety‐like behaviors, measured by open‐field, elevated plus maze, and light–dark box tests. In vivo multi‐electrode recordings awake were used examine neuronal activity. Chemogenetics silence specific brain regions. Viral tracing,...
ABSTRACT Background Low‐temperature cryopreservation is a common method for scientific research and clinical sample preservation when utilizing flow cytometry. In cytometry data analysis, traditional manual “gating” susceptible to past experience faces the challenge of subjective bias, time‐consuming, multidimensional analysis. With development algorithms, advantages dimensionality reduction clustering in result analysis are gradually becoming more prominent. Methods Flow was used detect...
Genomic imprinting is an epigenetic regulation that leads to gene expression in a parent-of-origin specific manner. AFF3, the central component of Super Elongation Complex-like 3 (SEC-L3), enriched at both intergenic-differentially methylated region (IG-DMR) and Meg3 enhancer within imprinted Dlk1-Dio3 locus regulate allele-specific this locus. The localization AFF3 IG-DMR requires ZFP57. However, how functions maintaining remains unclear. Here, we demonstrate associated with Krüppel-like...
Commonly encountered nontraumatic, moderate noise is increasingly implicated in anxiety; however, the neural substrates underlying this process remain unclear.We investigated circuit mechanism through which chronic exposure to moderate-level causes anxiety-like behaviors.Mice were exposed chronic, white [85 decibel (dB) sound pressure level (SPL)], 4 h/d for wk induce behaviors, assessed by open field, elevated plus maze, light-dark box, and social interaction tests. Viral tracing,...
The purpose of this study was to investigate the therapeutic effects hyperoside (Hyp) on glomerulosclerosis in diabetic nephropathy and its underlying mechanisms. Blood glucose, kidney mass, renal function mice were measured. Renal morphology observed using hematoxylin eosin, periodic acid - Schiff's, Masson's trichrome stain. Fibronectin (FN) collagen IV (COL IV) determined by Western blot immunohistochemical studies. Matrix metalloproteinases (MMP)-2 -9 tissue inhibitors metalloproteinase...
Chronic hepatitis B virus (HBV) infection can lead to liver cirrhosis and hepatocellular carcinoma. HBV reactivation during or after chemotherapy is a potentially fatal complication for cancer patients with chronic infection. Transcription of critical intermediate step the life cycle. However, factors controlling transcription remain largely unknown. Here, we found that different P-TEFb complexes are involved in viral genome. Both BRD4 super elongation complex (SEC) bind The treatment...
Pseudomonas aeruginosa biofilms employ a variety of strategies to hijack the host immune defense system achieve chronic infection. However, bacterial components that are involved in this process not yet fully understood. PcrV, needle tip protein P. type III secretion (T3SS), was downregulated during biofilm The impaired expression pcrV gene is associated with attenuated activation and an increased percentage M2 macrophages following Treatment exogenous PcrV produced from Escherichia coli...
is one of the most common nosocomial pathogens worldwide, known for its virulence, drug resistance, and elaborate sensor-response network. The primary challenge encountered by during initial stages infection immune clearance arising from host. resident macrophages barrier organs serve as frontline defense against these pathogens. Central to our understanding mechanism which bacteria modify their behavior circumvent macrophage-mediated clearance, ensuring persistence colonization. To...
Transposable elements (TEs) compose about 40% of the murine genome. Retrotransposition active TEs such as LINE-1 (L1) tremendously impacts genetic diversification and genome stability. Therefore, transcription transposition activities retrotransposons are tightly controlled. Here, we show that Krüppel-like zinc finger protein Zfp281 directly binds suppresses a subset retrotransposons, including young L1 repeat elements, in mouse embryonic stem (ES) cells. In addition, find Zfp281-regulated...
Smart wearable devices have become pervasive and are playing a more important role in our everyday lives. However, the small screen size very few buttons make interaction control cumbersome inconvenient. Previous solutions to mitigate this problem either require extra dedicated hardware, or instrument user's fingers with special purpose sensors, limiting their real-life applications. We present iDial, novel real time approach that enables virtual dial plate on hand back, extending beyond of...
Metformin is the first-line pharmacotherapy for type 2 diabetes mellitus (T2D). exerts its glucose-lowering effect primarily through decreasing hepatic glucose production (HGP). However, precise molecular mechanisms of metformin remain unclear due to supra-pharmacological concentration used in study. Here, we investigated role Foxo1 action control homeostasis and mechanism via transcription factor mice, as well clinical relevance with co-treatment aspirin. We showed that inhibits HGP blood a...
Hyperoside, a flavonoid isolated from conventional medicinal herbs, has been demonstrated to exert significant protective effect in diabetic nephropathy. This study aimed determine the underlying mechanisms, by which hyperoside inhibits high glucose-(HG-) induced proliferation mouse renal mesangial cells. Mouse glomerular cells line (SV40-MES13) was used inhibitory of on cell 30 mM glucose, simulate condition. Viable count assessed using Cell Counting Kit-8 and 5-ethynyl-20-deoxyuridine...
Activation of the NLRP3 inflammasome is critical in inflammatory response to gout. Potassium ion (K+) efflux mediated by TWIK2 channel an important upstream mechanism for activation. Therefore, may be a promising therapeutic target MSU crystal-induced inflammation. In present study, we investigated effect ML335, known K2P modulator, on responses and its underlying molecular mechanisms. By docking, calculated binding energies inhibition constants five modulators (Hydroxychloroquine,...
Hepatocellular carcinoma has high incidence and mortality worldwide. Liver is the site of most metabolic biotransformation, which could reflect status cells. Most plasma apolipoproteins, endogenous lipids lipoproteins are synthesized in liver. Therefore, effects lipid metabolites on prognosis HCC deserved to be explored. We prospectively included 58 healthy donors (HD), 50 chronic hepatitis (CH) patients a training cohort 189 with who underwent curative resections at Zhongshan Hospital from...
Cullin 4 (Cul4), a member of the evolutionally conserved cullin protein family, serves as scaffold to assemble multisubunit ubiquitin E3 ligase complexes. Cul4 interacts with Ring finger-containing ROC1 through its C-terminal domain and substrate recruiting subunit(s) N-terminus. Previous studies have demonstrated that ubiquitylates key regulators in cell cycle control mediates their degradation proteasomal pathway, thus contributing genome stability. Recent from several groups revealed can...
Upregulation of the neuropeptide neurotensin (NTS) in a subgroup lung cancers has been linked to poor prognosis. However, regulatory pathway centered on NTS cancer remains unclear. Here we identified NTS-specific enhancer adenocarcinoma cells. The AF4/FMR2 (AFF) family protein AFF1 occupies and inhibits transcription. Clustering analysis gene expression data demonstrated that is highly positively correlated with oncogenic factor CPS1. Detailed analyses IL6 antagonizes regulating Thus, our...
The aim of this study was to establish a differential high-resolution melting analysis for the detection K-ras codons 12 and 13 mutations in pancreatic cancer tissue.We tested sensitivity genotyping approach cell lines with known using 163- 59-base pair (bp) amplicons spanning 13. We then screened 50 tissues, which were subsequently sequenced confirm discovered 13.High-resolution more sensitive detecting than standard sequencing able reliably detect as little 10% mutant line DNA diluted...
The super elongation complex (SEC) containing positive transcription factor b plays a critical role in regulating elongation. AFF1 and AFF4, two members of the AF4/FMR2 family, act as central scaffold proteins SEC are associated with various human diseases. However, their precise roles transcriptional control remain unclear. Here, we investigate differences genomic distribution patterns AFF4 around start sites (TSSs). mainly binds upstream TSS, while is enriched downstream TSS. Notably,...