A5003631590- Gout, Hyperuricemia, Uric Acid
- Inflammasome and immune disorders
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Autophagy in Disease and Therapy
- Pancreatitis Pathology and Treatment
- RNA Research and Splicing
- Sirtuins and Resveratrol in Medicine
- Pancreatic and Hepatic Oncology Research
- Genomics and Chromatin Dynamics
- Viral Infections and Vectors
- Natural product bioactivities and synthesis
- Sepsis Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Retinoids in leukemia and cellular processes
- Phagocytosis and Immune Regulation
- Erythrocyte Function and Pathophysiology
- Machine Learning in Healthcare
- NF-κB Signaling Pathways
- Dietary Effects on Health
- Theological Perspectives and Practices
- Spondyloarthritis Studies and Treatments
- Veterinary medicine and infectious diseases
- Enhanced Recovery After Surgery
- Lysosomal Storage Disorders Research
- Cellular transport and secretion
North Sichuan Medical University
2014-2025
Affiliated Hospital of North Sichuan Medical College
2018-2025
Albert Einstein College of Medicine
2007-2024
Nanchong Central Hospital
2020-2022
Huizhou Central People's Hospital
2021
Southern Medical University
2021
Nanfang Hospital
2021
Shandong University
2004-2020
Chinese Academy of Medical Sciences & Peking Union Medical College
2020
Qilu Hospital of Shandong University
2014-2019
Rates of autophagy, the mechanism responsible for lysosomal clearance cellular components, decrease with age. We have previously described an age-related decline in chaperone-mediated autophagy (CMA), a selective form by which particular cytosolic proteins are delivered to lysosomes after binding lysosome-associated membrane protein type 2A (LAMP-2A), receptor this pathway. CMA age because levels LAMP-2A. In work we investigated reasons reduced LAMP-2A While transcriptional rates remain...
Sirtuins [sirtuin (SIRT)1-SIRT7] mediate the longevity-promoting effects of calorie restriction in yeast, worms, flies, and mice. Additionally, SIRT3 is only SIRT analog whose increased expression has been shown to be associated with longevity humans. The polyphenol resveratrol (RSV) first compound discovered able mimic by stimulating SIRTs. In present study, we report that RSV activated cardiac fibroblasts both vivo vitro. Moreover, wild-type mice, prevented hypertrophy response...
Gouty arthritis is characterized by the deposition of monosodium urate (MSU) within synovial joints and tissues due to increased concentrations. In this study, we explored effect natural compound curcumin on MSU crystal-stimulated inflammatory response. THP-1-derived macrophages murine RAW264.7 were pretreated with for 1 h then stimulated suspensions 24 h. The protein level TLR4, MyD88, IκBα, activation NF-κB signaling pathway, expression downstream cytokines, activity NLRP3 inflammasome...
Chaperone-mediated autophagy (CMA) is part of the mammalian cellular proteostasis network that ensures protein quality control, maintenance proteome homeostasis, and changes required for adaptation to stress. Loss one hallmarks aging. CMA decreases with age in multiple rodent tissues human cell types. A decrease lysosomal levels lysosome-associated membrane type 2A (LAMP2A), receptor, has been identified as a main reason declined Here, we report constitutive activation calorie restriction...
Background The toll-like receptor (TLR)4-interleukin1β (IL1β) signaling pathway is involved in the monosodium urate (MSU)-mediated inflammation. aim of this present study was to determine whether TLR4 gene rs2149356 SNP associated with gouty arthritis (GA) susceptibility and impacts TLR4-IL1β molecules expression. Methods Findings detected 459 GA patients 669 control subjects (containing healthy 210 hyperuricemic subjects). Peripheral blood mononuclear cells (PBMCs) mRNA serum IL1β were...
Acute gout is an inflammatory response induced by monosodium urate (MSU) crystals. HSP60 a highly conserved stress protein that acts as cellular “danger” signal for immune reactions. In this study, we aimed to investigate the role and molecular mechanism of in gout. expression was detected peripheral blood mononuclear cells (PBMCs) plasma patients. The effect were studied MSU crystals treatment macrophages C57BL/6 mice. JC-1 probe MitoSOX Red used measure mitochondrial membrane potential...
Alzheimer's disease (AD) is the most prevalent neurodegenerative disorder, but no drugs can cure this disease. Chalcones possess good antioxidant activity, anti-neuroinflammatory neuroprotective effects, inhibitory effects on Aβ aggregation, and disaggregation ability. Therefore, chalcones are ideal lead compounds, discovery of novel anti-AD agent-based necessary. Hydroxy groups aryl benzyl ether were introduced into chalcone scaffolds to obtain a series 2-hydroxyl-4-benzyloxy derivatives....
Previous studies have confirmed that alcohol can increase the sensitivity of pancreas to stressors and exacerbate severity pancreatitis when excessive intake is combined with other causes. In current work, this study attempted explore how does regulate cerulein-induced acute pancreatitis, especially before inflammation occurs. Proteomics was performed analyze differentially expressed proteins in pancreatic tissues from a rat model pancreatitis. The metabolite levels tissue, serum rats...
Asthma is a chronic inflammatory disease that cannot be cured. Maresin 1 (MaR1) specific lipid synthesized by macrophages exhibits powerful anti-inflammatory effects in various diseases. The goal of this study was to evaluate the effect MaR1 on allergic asthma using an ovalbumin- (OVA-) induced model. Thirty BALB/c mice were randomly allocated control, OVA, and + OVA groups. Mice sacrificed 24 hours after end last challenge, serum, bronchoalveolar lavage fluid (BALF), lung tissue collected...
Piwi proteins are required for germ cell proliferation, differentiation, and line stem maintenance. In normal tissues, human mouse Piwil2 primarily expressed in testis but widely tumors. However, the underlying mechanism remains largely unknown. vertebrates, transforming growth factor (TGF)-β signaling plays an important role patterning embryo control of differentiation. A previous study has shown a Zili, gene zebrafish, cells zebrafish. Here we report that zili functions early inhibits...
Glut9 is highly expressed in the human kidney proximal convoluted tubular and plays a crucial role regulation of plasma urate levels. The gene effects were stronger among women. Our results show that 17-β-estradiol (E2) through ER (estrogen receptor) β downregulates protein expression on renal epithelial cell line (HK2). Intriguingly, E2 does not affect mRNA. ERβ linked to PTEN, PTEN negatively regulates PI3K/AKT pathway, pathway inhibition may lead autophagy. Further study indicates though
Excessive generation of reactive oxygen species (ROS) has great impacts on MSU crystal-induced inflammation. Drp1-dependent mitochondrial fission is closely associated with ROS levels. However, whether Drp1 signaling contributes to inflammation remains unclear. Mice bone marrow-derived macrophages (BMDMs) were primed LPS and then stimulated suspensions for 12 h. The protein levels dynamics, oxidative stress, mitophagy detected by Western blot. BMDMs loaded MitoTracker Green probe detect...
The RNA-binding protein tristetraprolin (TTP) is an anti-inflammatory factor that prompts the mRNA decay of target mRNAs and involved in inflammatory diseases such as rheumatoid arthritis (RA). TTP regulated by phosphorylation, phosphatase 2A (PP2A) can dephosphorylate to activate its mRNA-degrading function. Some small molecules enhance PP2A activation. Short interfering RNA (siRNA) targeting expression or agonist (Arctigenin) was administered monosodium urate (MSU) crystal-induced J774A.1...
Activation of the NLRP3 inflammasome is critical in inflammatory response to gout. Potassium ion (K+) efflux mediated by TWIK2 channel an important upstream mechanism for activation. Therefore, may be a promising therapeutic target MSU crystal-induced inflammation. In present study, we investigated effect ML335, known K2P modulator, on responses and its underlying molecular mechanisms. By docking, calculated binding energies inhibition constants five modulators (Hydroxychloroquine,...
The Dazl gene encodes a germ-cell-specific RNA-binding protein which is essential for spermatogenesis. It has been proposed that this (DAZL) binds to RNA in the cytoplasm of germ cells and controls Using specific nucleic acids associated with proteins (SNAAP) technique, we identified 17 target mRNAs bound by mDAZL. Among these transcripts, focused on TSSK2, testis-specific serine/threonine kinase. To date, five TSSK family members have cloned, all are exclusively expressed testis. We...
Dnd (dead end) gene encodes an RNA binding protein and is specifically expressed in primordial germ cells (PGCs) as a vertebrate-specific component of the plasma throughout embryogenesis. By utilizing technique specific nucleic acids associated with proteins (SNAAP), 13 potential target mRNAs zebrafish (ZDnd) were identified from 8-cell embryo, 8 have been confirmed using RT-PCR analysis. Of mRNAs, present study focused on regulation geminin, which inhibitor DNA replication. Using...
Sepsis is a life-threatening disease with high mortality and expensive cost of treatment.In order to improve the outcomes patients, it important detect atrisk patients sepsis at an early stage.The Phys-ioNet/Computing in Cardiology Challenge 2019 focused on improving predicting six hours before clinical diagnosis by using latest definition Sepsis-3.A total 40,336 ICU were provided as public training data, A hidden test dataset was used evaluate.An ensemble model, which combined boosting...