A5026718106- Neonatal Respiratory Health Research
- MicroRNA in disease regulation
- Cystic Fibrosis Research Advances
- Congenital heart defects research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cancer-related molecular mechanisms research
- Zebrafish Biomedical Research Applications
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Pluripotent Stem Cells Research
- Congenital Heart Disease Studies
- Down syndrome and intellectual disability research
- Chromosomal and Genetic Variations
- Acute Lymphoblastic Leukemia research
- Renal and related cancers
- Reproductive System and Pregnancy
- Birth, Development, and Health
- Advanced biosensing and bioanalysis techniques
- Acute Myeloid Leukemia Research
- RNA and protein synthesis mechanisms
- Neurobiology and Insect Physiology Research
- Craniofacial Disorders and Treatments
- Carbon and Quantum Dots Applications
- RNA Research and Splicing
Fudan University
2024-2025
University of Hong Kong
2015-2025
Sichuan University
2016-2025
Chinese University of Hong Kong
2012-2025
Zhongshan Hospital of Xiamen University
2024-2025
West China Second University Hospital of Sichuan University
2012-2024
Second Affiliated Hospital of Chengdu University of Traditional Chinese
2024
Affiliated Hospital of Qingdao University
2020-2021
Wuhan University of Technology
2021
Qingdao University
2020-2021
MicroRNAs (miRNAs) are short non-coding RNA molecules playing regulatory roles by repressing translation or cleaving transcripts. Recent studies indicate that miRNAs mechanistically involved in the development of mammalian spermatogenesis. However, little work has been done to compare miRNA expression patterns between immature and mature mouse testes. Here, we employed a microarray detect 892 order evaluate miRNA. The 19 was significantly different individuals. Fourteen were upregulated five...
Do microRNAs (miRNAs) contribute to aberrant progesterone receptor (PGR) expression in the eutopic endometrium of women with endometriosis? miR-196a upregulates MEK/ERK signalling, mediating a downregulation PGR minimal or mild endometriosis. Implantation failure is strongly suggested as an underlying cause for observed infertility Progesterone resistance, which mainly caused by aberrantly expressed endometrium, considered key factor decreased endometrial receptivity; thus far, epigenetics,...
Piwi proteins and Piwi-interacting RNAs (piRNAs) have been implicated in transposon control germline from Drosophila to mammals. To examine the profile of small RNA expression human cancer cells explore difference transcriptome, libraries prepared wildtype, HILI overexpressed knockdowned Hela were sequenced using Solexa technology. piRNAs other repeat-associated observed cells. By situ hybridization, piR-49322 was localized nucleolus around periphery nuclear membrane Following overexpression...
Abstract Background: The CUG-binding protein Elav-like family member 2 ( CELF2 ) gene has been linked to the pathogenesis of epilepsy, but its precise role remains unclear. This study aimed investigate pathogenic mechanisms mutation in utilizing zebrafish models explore molecular pathways and biological impact. Methods: Whole-exome sequencing was performed identify mutations associated with epilepsy. model were generated using clustered regularly interspaced short palindromic repeats...
Abstract Mutations of cystic fibrosis transmembrane conductance regulator (CFTR) cause (CF) with a multitude clinical manifestations. Some CF patients develop clinically significant anemia, suggesting that CFTR may regulate hematopoiesis. Here, we report cftr mutant zebrafish model exhibits primitive and definitive hematopoietic defects impaired Wnt signaling. Cftr is found to interact, via its PDZ-binding domain (PDZBD), Dishevelled (Dvl), key component signaling required for progenitor...
Emerging evidence has shown that the N6-methyladenosine (m6A) modification of RNA plays key roles in tumorigenesis and progression various cancers. However, potential m6A long noncoding RNAs (lncRNAs) pancreatic cancer (PaCa) are still unknown. To analyze prognostic value m6A-related lncRNAs PaCa, an lncRNA signature was constructed as a risk model via Pearson's correlation univariate Cox regression analyses The Cancer Genome Atlas (TCGA) database. tumor microenvironment (TME), mutation...
ABSTRACT Objective Emerging evidence highlights the involvement of long non‐coding RNAs (lncRNAs) and ferroptosis in pathogenesis traumatic brain injury (TBI). However, regulatory role lncRNAs TBI‐induced remains poorly understood. This study aims to investigate a specific lncRNA, noncoding transcript chemokine (C‐C motif) ligand 4 ( Ccl4 ) overlapping Ntoco ), regulation following TBI explore its potential as therapeutic target. Methods The expression levels controlled cortical (CCI) mice...
The piwi-like 2 (piwil2) gene is widely expressed in tumors and protects cells from apoptosis induced by a variety of stress stimuli. However, the role Piwil2 Fas-mediated remains unknown. Here, we present evidence that inhibits apoptosis. By bacterial two-hybrid screening, identify new Piwil2-interacting partner, keratin 8 (K8), major intermediate filament protein protecting cell Our results show binds to K8 p38 through its PIWI domain forms Piwil2/K8/P38 triple protein-protein complex....
Abstract Background Reptiles exhibit a wide variety of skin colors, which serve essential roles in survival and reproduction. However, the molecular basis these conspicuous colors remains unresolved. Results We investigate color morph-enriched Asian vine snakes ( Ahaetulla prasina ), to explore mechanism underpinning variations. Transmission electron microscopy imaging metabolomics analysis indicates that chromatophore morphology (mainly iridophores) is main for differences color....
PIWIL2, called HILI in humans, is a member of the PIWI subfamily. This subfamily has highly conserved PAZ and Piwi domains implicated several critical functions, including embryonic development, stem-cell self-renewal, RNA silencing, translational control. However, underlying molecular mechanism remains largely unknown. Transforming growth factor-β (TGF-β) secreted multifunctional protein that controls developmental processes pathogenesis many diseases. TGF-β signaling activated by...
Piwi proteins are required for germ cell proliferation, differentiation, and line stem maintenance. In normal tissues, human mouse Piwil2 primarily expressed in testis but widely tumors. However, the underlying mechanism remains largely unknown. vertebrates, transforming growth factor (TGF)-β signaling plays an important role patterning embryo control of differentiation. A previous study has shown a Zili, gene zebrafish, cells zebrafish. Here we report that zili functions early inhibits...
Abstract Potential risks of treatment with hydroxychloroquine (HCQ) include QT interval prolongation, hypoglycemia, a wide range neuropsychiatric manifestations, hematotoxicity, and potential genetic defects. HCQ is extremely toxic when used in overdose can lead to tachycardia, hypotension, known central nervous system, transmission defects, hypokalemia other manifestations individuals. The mechanism excessive leading these still unclear. In this paper, at different concentrations was treat...
Endothelial cell‐specific molecules (ECSMs) play a pivotal role in the pathogenesis of many angiogenesis‐related diseases. Since its initial discovery, exact function human ECSM2 has not been defined. In this study, by database mining, we identified number hypothetical proteins across species exhibiting substantial sequence homology to ECSM2. We showed that is preferentially expressed endothelial cells and blood vessels. Their characteristic structures unique expression patterns suggest an...
The toxicity of CDs in mice and zebrafish the potential relationship between autophagy was evaluated.
In this study, the expression of PTAR in cervical cancer tissues and cells was quantified by real-time PCR. Then, roles HeLa cell proliferation cycle were analyzed a CCK-8 assay flow cytometry, respectively.The effects on migration invasion checked Transwell wound healing assays.The effect apoptosis using annexin V/FITC staining. Finally, interaction between miR-101 uterine verified through dual-luciferase reporter correlation analysis. The results showed that aberrantly ascended lines...
The Dazl gene encodes a germ-cell-specific RNA-binding protein which is essential for spermatogenesis. It has been proposed that this (DAZL) binds to RNA in the cytoplasm of germ cells and controls Using specific nucleic acids associated with proteins (SNAAP) technique, we identified 17 target mRNAs bound by mDAZL. Among these transcripts, focused on TSSK2, testis-specific serine/threonine kinase. To date, five TSSK family members have cloned, all are exclusively expressed testis. We...
Cystic fibrosis transmembrane conductance regulator (CFTR) is classified as an anion channel transporter of Cl- and HCO3-. Through interactions with its PDZ domain, CFTR capable regulating other proteins, such protein phosphatase 2A (PP2A). The aberrant expression mutation have been observed in several tumor, but not philadelphia chromosome-positive(Ph+) acute leukemia, including Ph+ B cell lymphoblastic leukemia(Ph+ B-ALL) chronic myelogenous leukemia blast crisis phases (CML-BC). In this...
ABSTRACT Observational studies have found that elevated serum homocysteine (Hcy) levels during pregnancy may be associated with the occurrence of neural tube defects (NTDs). However, effect Hcy on fetal development and its underlying molecular mechanisms remains unclear. To uncover mechanism, we analyzed concentration in pregnant women normal abnormal outcomes treated zebrafish model embryos high Hcy. Our findings indicate are adverse outcomes. Using transcriptome analysis, led to...